SKU: 16-1316
{"url":"https://www.epicypher.com/products/nucleosomes/recombinant-nucleosomes-complete-list/nucleosome-recombinant-human-h3k4me3-dnuc-non-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":768,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)","Nucleosomes/Recombinant Nucleosomes (Complete List)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/768/966/nucleosome-recombinant-human-h3k4me3-dnuc-nonbiotinylated__98947.1645734558.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K4me3 dNuc, Non‑biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=768","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"859","name":"Pack Size","value":"50 µg"}],"sku":"16-1316","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n Mononucleosomes assembled from recombinant human histones expressed\n in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4;\n accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805)\n wrapped by 147 base pairs of 601 positioning sequence DNA. Histone\n H3 (created by a proprietary semi-synthetic method) contains\n trimethyl-lysine at position 4. The nucleosome is the basic subunit\n of chromatin. The 147 bp 601 sequence, identified by Lowary and\n Widom [1], has high affinity for histone octamers and is useful for\n nucleosome assembly.\n </p>\n <p>*H3K4me3 has a Cys to Ala substitution at position 110.</p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-1316-protein-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-1316-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-1316-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Protein Gel Data </strong><br />\n Coomassie stained PAGE gel of proteins in H3K4me3 dNuc (1\n µg) to demonstrate the purity of histones in the\n preparation. Sizes of molecular weight markers and positions\n of the core histones (H2A, H2B, H3K4me3 and H4) are\n indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-1316-dna-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-1316-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-1316-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: DNA Gel Data </strong><br />\n H3K4me3 dNuc resolved via native PAGE gel and stained with\n ethidium bromide to visualize DNA.\n <strong>Lane 1:</strong> Free DNA (Catalog No.\n <a\n href=\"/products/nucleosomes/nucleosome-components-and-subunits/nucleosome-assembly-601-sequence-dna\"\n >18-0006</a\n >; 100 ng). <strong>Lane 2:</strong> Intact nucleosomes (400\n ng).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-1316-mass-spec-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"16-1316-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-1316-mass-spec-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Mass Spec Data </strong><br />\n Synthetic H3K4me3 histone analyzed by high resolution mass\n spectrometry. Expected mass = 15,267.77 Da. Determined mass\n = 15,266.47 Da.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-1316-western-blot-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"16-1316-western-blot-data\"\n src=\"/content/images/products/nucleosomes/16-1316-western-blot-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 4: Western Blot Data </strong><br />\n Western Analysis of H3K4me3 dNuc.\n <strong>Top Panel:</strong> Unmodified H3 nucleosomes\n (Catalog No\n <a\n href=\"/products/nucleosomes/mononucleosomes-recombinant-human\"\n >16-0009</a\n >; Lane 1) and H3K4me3 nucleosomes (Lane 2) were probed with\n an anti-H3K4me3 antibody and analyzed via ECL readout. Only\n the H3K4me3 sample produced a detectable signal.\n <strong>Bottom Panel:</strong> Detail from Coomassie stained\n gel showing unmodified nucleosomes (Lane 1) and H3K4me3\n nucleosomes (Lane 2).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"16-1316-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-1316-protein-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"16-1316_dna_gel-data\"\n src=\"/content/images/products/nucleosomes/16-1316-dna-gel-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"16-1316-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-1316-mass-spec-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"16-1316-western-blot-data\"\n src=\"/content/images/products/nucleosomes/16-1316-western-blot-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n H3K4me3 dNuc (27.3 µg protein weight, 50.0 µg DNA + protein) in\n 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20%\n glycerol. MW = 199,250.1.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six (6) months at -80°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n H3K4me3 dNuc is highly purified and suitable for use as a substrate\n in enzyme screening assays or for effector protein binding\n experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n target=\"_blank\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n >9514715</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-1316.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":296,"sku":null,"name":"Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k4me3-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)","Nucleosomes/Modified Designer Nucleosomes (dNucs™)/dNuc - Smaller Pack Size Available"],"summary":"\n \n \n Species: Human\n \n \n Source: E. coli & synthetic DNA\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/296/283/dNucs__33084.1569012494.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/296/275/3_Nucleosome__08589.1569012494.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/296/283/dNucs__33084.1569012494.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated"}],"date_added":"11th May 2016","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":null,"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$295.00","value":295},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=296"},{"id":596,"sku":"16-0335","name":"Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k4me3-k9-14-18ac-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/596/545/Recombinant_nucleosome__16505.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/596/545/Recombinant_nucleosome__16505.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac dNuc, Biotinylated"}],"date_added":"23rd Aug 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":394,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=596","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=596"},{"id":536,"sku":"16-1362","name":"Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Non-Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3cit2-8-17-dnuc-non-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":"\n \n \n Species: Human\n \n \n Source: E. coli & synthetic DNA\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/536/384/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__15131.1525791275.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Non-Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/536/384/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__15131.1525791275.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Non-Biotinylated"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/536/1127/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__39402.1680823466__39580.1681143400.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3Cit2,8,17 dNuc, Non-Biotinylated"}],"date_added":"7th May 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":793,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=536","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=536"},{"id":605,"sku":"16-0358","name":"Nucleosome, Recombinant Human, H4R3me2s dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h4r3me2s-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/605/554/Recombinant_nucleosome__90101.1538135197.png?c=2","alt":"Nucleosome, Recombinant Human, H4R3me2s dNuc, 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Pack Size: 50 µg
