Ultra-sensitive, low-input chromatin mapping assays
CUTANA® ChIC / CUT&Tag assays build on recent advancements in immunotethering technology to deliver a highly sensitive and cost-effective workflow for epigenomic mapping. There are numerous advantages to CUT&Tag over ChIP-seq, the standard chromatin profiling assay:
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CUTANA® CUT&Tag provides impressive gains
compared to ChIP-seq,
the leading assay for chromatin and histone PTM profiling
How does CUT&Tag work?
Cleavage Under Targets and Tagmentation (CUT&Tag) is a novel immunotethering assay, based on the technology used in Chromatin ImmunoCleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods.
In CUT&Tag, a fusion of protein A, protein G, and Tn5 transposase is used to catalyze simultaneous cleavage and sequencing adapter ligation at antibody-bound chromatin. The use of Tn5 in CUT&Tag is a key advance, as this step eliminates the need for chromatin fragmentation as well as expensive library preparation.
The result is a highly cost- and time-efficient NGS assay, with improved signal : noise and genomic coverage vs. ChIP-seq.
Quality data with lower input and sequencing depth requirements
CUTANA® CUT&Tag generates similar results at a fraction of the cell input and sequencing depth used in ChIP-seq. A representative 300 kb region at the LAMC3 gene is shown for CUT&Tag (orange), CUT&RUN (green) and ChIP-seq (blue) data generated using H3K4me3 and H3K27me3 antibodies (EpiCypher 13-0041 and 13-0030, respectively). Rabbit IgG Negative Control Antibody (EpiCypher 13-0042) and ChIP Input control are shown for comparison (control tracks are scaled to the track with the highest signal in each approach).
Go from cells to sequencing-ready DNA in < 2 days
Get started with CUTANA® CUT&Tag assays
CUTANA® pAG-Tn5 for ChIC / CUT&Tag
Plan your next CUT&Tag experiment! pAG-Tn5 is the essential enzyme for CUT&Tag assays. Available in 50 or 250 reaction sizes.
The CUTANA® CUT&Tag Protocol
In addition to pAG-Tn5, EpiCypher offers many supporting products to help you get started with CUT&Tag assays, such as antibodies, experimental controls, magnetic beads, and more!
We have developed a streamlined, user-friendly CUT&Tag protocol, validated for:
- Histone PTMs and select validated targets
- Low cell inputs (down to 1,000 cells, without adaptions to protocol)
- Reduced sequencing depths (3-5 million reads / sample)
- High-throughput sample handling
The CUT&Tag protocol also contains a thorough FAQ section, in which we address the most common questions about the CUT&Tag assay. For more information on our protocol and how to set up your own assay, read our blog and our CUTANA CUT&Tag brochure.