SKU: 16-0009
{"url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":246,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant human nucleosomes for enzyme screening assays","category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/246/332/Human_recombinant_nucleosome__44913.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=246","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1302","name":"Pack Size","value":"100 μg"},{"id":"1303","name":"Internal Comment","value":"Bulk in LG"},{"id":"1304","name":"Internal Comment","value":"Additional Units Bottom Right of Psylocke"}],"sku":"16-0009","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong><i>E. coli</i> & synthetic DNA\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Tag: </strong>None\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>MW: </strong>199,260.8 Da\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\" style=\"display: block\">\n <p>\n Recombinant mononucleosomes (rNucs) consist of 147 base pairs of DNA wrapped around an octamer of core\n histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of\n chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone\n octamers and is useful for nucleosome assembly.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\" style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/16_0009_DNA_web.jpg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"16-0009 DNA Gel\"\n src=\"/content/images/products/nucleosomes/16_0009_DNA_web.jpg\" class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: DNA gel data\n </strong><br />Recombinant Mononucleosomes resolved via native PAGE gel and stained with ethidium\n bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (EpiCypher <a\n href=\"/products/nucleosomes/nucleosome-components-and-subunits/nucleosome-assembly-601-sequence-dna\"\n target=\"_blank\">18-0006</a>; 200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400\n ng).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/16_0009_protein_web.jpg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"16-0009 Protein Gel\"\n src=\"/content/images/products/nucleosomes/16_0009_protein_web.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: Protein gel data </strong><br />\n Coomassie stained PAGE gel of proteins in Recombinant Mononucleosomes (0.5 µg) demonstrates the purity of histones in the preparation. Sizes of\n molecular weight markers and positions of the core histones (H2A, H2B, H3, and H4) are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/16_0009_hmtase_web.jpg\" target=\"_blank\"\n class=\"image-picker__main-image-link\">\n <img loading=\"lazy\" alt=\"16-0009 HMTase Assay\"\n src=\"/content/images/products/nucleosomes/16_0009_hmtase_web.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: HMTase assay data </strong><br />\n Recombinant Mononucleosomes used as a substrate in a\n radioactive histone methyltransferase assay with (+SET8) or\n without (-SET8) recombinant SET8, which is an HMTase that\n requires nucleosomal substrates for activity.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\" alt=\"16-0009 DNA Gel\" src=\"/content/images/products/nucleosomes/16_0009_DNA_web.jpg\"\n width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" />\n <img loading=\"lazy\" alt=\"16-0009 Protein Gel\"\n src=\"/content/images/products/nucleosomes/16_0009_protein_web.jpg\" class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\" alt=\"16-0009 HMTase Assay\"\n src=\"/content/images/products/nucleosomes/16_0009_hmtase_web.jpg\" class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For\n best results, aliquot and avoid freeze/thaws.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p>\n 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (54.6 µg protein, 100 µg DNA +\n protein).\n </p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n rNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme\n assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein\n interaction assays, structural studies, and in effector protein binding experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)<br />\n H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)<br />\n H3.1 - P68431 (alt. names: H3, H3/a, H3/b, H3/c, H3/d)<br />\n H4 - P62805\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J Mol. Biol.</em> (1998). PMID:\n <a href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\" target=\"_blank\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\">9514715</a><br />\n <strong>Product References:</strong>\n <iframe class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shrgBfbgAuWF6U5Yt?backgroundColor=purple&viewControls=on\" frameborder=\"0\"\n onmousewheel=\"\" width=\"100%\" height=\"300\" style=\"background: transparent; border: 1px solid #ccc\"></iframe>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/16-0009.pdf\" target=\"_blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0009 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$425.00","value":425,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Recombinant human nucleosomes for enzyme screening assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":84,"sku":"16-0006","name":"Mononucleosomes, Recombinant Human Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)"],"summary":"\n \n \n \n Description\n \n \n Mononucleosomes ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/84/331/Human_recombinant_nucleosome__14471.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/84/331/Human_recombinant_nucleosome__14471.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human Biotinylated"}],"date_added":"3rd Dec 2014","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1251,"name":"Pack Size","value":"50 μg"},{"id":1252,"name":"Internal Comment","value":"Has Bulk - Not Included in Amount"},{"id":1253,"name":"Internal Comment","value":"65 extra units located bottom-right of Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=84","price":{"without_tax":{"currency":"USD","formatted":"$475.00","value":475},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=84"}],"shipping_messages":[],"rating":0,"meta_keywords":"nucleosome, recombinant nucleosome, human nucleosome, chromatin, histone","show_quantity_input":1,"title":"Mononucleosomes, Recombinant Human","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/246/332/Human_recombinant_nucleosome__44913.1516390310.gif?c=2","alt":"Mononucleosomes, Recombinant Human"}]}
Pack Size: 100 μg
