CUTANA™ pAG-Tn5 for CUT&Tag
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":764,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"The key enzyme for performing CUT&Tag, an ultra-sensitive chromatin mapping assay with significant advantages over ChIP-seq.","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=764","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1005","name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"sku":"15-1017","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Transposase\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Mol Wgt: </strong>191 kDa\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong><em>E. coli</em>\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag: </strong>None\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div\n id=\"w-s-3835-15-1017\"\n style=\"\n width: max-content;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-15-1017-div\">\n <a\n id=\"bioz-w-pb-15-1017\"\n style=\"font-size: 12px; color: transparent\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n </a></div\n ></a>\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" style=\"display: block\">\n <p>\n Available in 50 and 250 Reaction pack sizes. CUTANA pAG-Tn5 is the\n key reagent for performing efficient mapping of chromatin features\n in Cleavage Under Targets and Tagmentation (CUT&Tag) [<a href=\"https://pubmed.ncbi.nlm.nih.gov/31036827/\"\n target=\"_blank\" rel=\"noopener noreferrer\">Kaya-Okur et al., 2019</a>]. CUT&Tag offers significant\n improvements in signal to noise at\n reduced cell inputs and sequencing depth compared to ChIP-seq. As a\n fusion of Proteins A and G to a highly active\n <em>E. coli</em> transposase mutant (Tn5), CUTANA pAG-Tn5 is\n compatible with target antibodies from a broad spectrum of host\n species. It also comes without an epitope tag, making it suitable\n for tag-mediated CUT&Tag (e.g. FLAG, HA, TY1, V5, etc.). This\n product is highly purified to remove contaminating\n <em>E. coli</em> DNA, which can be tagmented during storage and\n complicate analysis at low cell numbers. For superior normalization\n as well as antibody validation and reaction monitoring,\n <a href=\"https://www.epicypher.com/products/nucleosomes/snap-cutana-spike-in-controls\">SNAP-CUTANA™\n nucleosome spike-ins</a>\n are recommended. The Tn5 comes charged with mosaic adapters and is\n ready to be used in CUT&Tag.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/proteins/15-1017-protein-gel-data.jpeg\" target=\"blank\"\n class=\"image-picker__main-image-link\"><img alt=\"15-1017-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1017-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: Protein gel data </strong><br />\n CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE\n and stained with Coomassie blue. The migration and molecular\n weight of the protein standards are indicated. Uncharged\n pAG-Tn5 monomer is 78.5 kDa, however once charged with DNA,\n Tn5 dimerizes to a final complex weight of 191 kDa.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/proteins/15-1017-size-distribution.jpeg\" target=\"blank\"\n class=\"image-picker__main-image-link\"><img alt=\"15-1017-size-distribution\"\n src=\"/content/images/products/proteins/15-1017-size-distribution.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: Size distribution of released\n chromatin</strong><br />\n CUT&Tag was performed as described above. Recovered DNA was\n directly PCR amplified to produce sequence-ready libraries.\n Agilent TapeStation traces for libraries derived from\n negative control Rabbit IgG (top) and H3K27me3 (bottom) are\n shown. Excised DNA is highly enriched for mononucleosomes\n (peak at ~300 bp reflects ~150 bp insert size).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/proteins/15-1017-cut-tag-data.jpeg\" target=\"blank\"\n class=\"image-picker__main-image-link\">\n <img alt=\"15-1017-cut-tag-data\" src=\"/content/images/products/proteins/15-1017-cut-tag-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: CUT&Tag data </strong><br />\n CUT&Tag was performed as described above. Representative\n sequencing tracks obtained using CUTANA pAG-Tn5 show a 232\n kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced\n clear peaks with genomic distribution profiles consistent\n with the known biological functions of H3K4me3 and H3K27me3\n as well as minimal background in the IgG negative control.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/methods/cut-and-tag-methods.png\" target=\"blank\"\n class=\"image-picker__main-image-link\">\n <img alt=\"cut-and-tag-methods\" src=\"/content/images/products/methods/cut-and-tag-methods.png\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>CUT&Tag methods</strong><br />\n CUT&Tag was performed on 100k K562 cells with 0.5 µg of\n either IgG (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\">13-0042</a>),\n H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\">13-0041</a>),\n or H3K27me3 (<a\n href=\"https://www.thermofisher.com/antibody/product/H3K27me3-Antibody-clone-G-299-10-Monoclonal/MA5-11198\">ThermoFisher\n MA5-11198</a>) antibodies using CUTANA™ pAG-Tn5 (1:20 dilution)\n following the EpiCypher Direct-to-PCR CUT&Tag\n <a href=\"/protocols\">protocol</a>. Libraries were run on an\n Illumina NextSeq2000 with paired-end sequencing (2x50 bp).\n Sample sequencing depth was 0.3 million reads (IgG), 1.7\n million reads (H3K4me3), and 3.8 million reads (H3K27me3).\n Data were aligned to the hg19 genome using Bowtie2. Data\n were filtered to remove duplicates, multi-aligned reads, and\n ENCODE DAC Exclusion List regions.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img alt=\"15-1017-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1017-protein-gel-data.jpeg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" />\n <img alt=\"15-1017-size-distribution\"\n src=\"/content/images/products/proteins/15-1017-size-distribution.jpeg\"\n class=\"image-picker__side-image\" role=\"button\" />\n <img alt=\"15-1017 C&T\" src=\"/content/images/products/proteins/15-1017-cut-tag-data.jpeg\"\n class=\"image-picker__side-image\" role=\"button\" />\n <img alt=\"cut-and-tag-methods\" src=\"/content/images/products/methods/cut-and-tag-methods.png\"\n class=\"image-picker__side-image\" role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Accessory Products</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA™ Concanavalin A Conjugated Paramagnetic Beads</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads\">21-1401</a>\n </td>\n </tr>\n\n <tr>\n <td>SNAP-CUTANA™ K-MetStat Panel</td>\n <td>\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\">19-1002</a>\n </td>\n </tr>\n\n <tr>\n <td>\n Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP\n </td>\n <td>\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\">13-0041</a>\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody</td>\n <td>\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\">13-0042</a>\n </td>\n </tr>\n\n <tr>\n <td>Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-tag-assays/anti-rabbit-secondary-antibody-for-cutana-cut-tag-workflows\">13-0047</a>\n </td>\n </tr>\n\n <tr>\n <td>Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutanac-cut-tag-assays/anti-mouse-secondary-antibody-for-cutana-cut-tag-workflows\">13-0048</a>\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-tag-assays/cutana-high-fidelity-2x-pcr-master-mix\">15-1018</a>\n </td>\n </tr>\n\n <tr>\n <td>Magnetic Separation Rack, 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\">10-0008</a>\n </td>\n </tr>\n\n <tr>\n <td>Magnetic Separation Rack, 1.5 mL Tubes</td>\n <td>\n <a href=\"/products/epigenetics-reagents-and-assays/magnetic-separation-rack-1-5-ml-tubes\">10-0012</a>\n </td>\n </tr>\n\n <tr>\n <td>CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes\">10-0009</a>\n </td>\n </tr>\n <tr>\n <td id=\"specific-row\">\n CUTANA™ Nuclei Extraction Buffer\n </td>\n <td>\n <a\n href=\"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-nuclei-extraction-buffer\">21-1026</a>\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for one year at -20°C from date of receipt. The protein\n is not subject to freeze/thaw under these conditions.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1%\n Triton X-100, 50% glycerol\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Adapters</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin-bottom: 0\">\n Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’\n </p>\n <p style=\"margin-bottom: 0\">\n Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’\n </p>\n <p style=\"margin-bottom: 0\">\n Tn5ME-rev: 5’-[phos]CTGTCTCTTATACACATCT-3’\n </p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Add 2.5 µL of the supplied enzyme to a 50 µL CUT&Tag reaction (20X\n dilution). For detailed applications and uses of this product,\n please see our\n <a href=\"https://www.epicypher.com/content/documents/protocols/cutana-cut&tag-protocol.pdf\">CUT&Tag\n protocol</a>.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/15-1017.pdf\" target=\"blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\">\n <g>\n <path class=\"product-documents__svg-pdf\" 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a href=\"/content/documents/protocols/cutana-cut&tag-protocol.pdf\" target=\"blank\"\n class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\">\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">CUT&Tag Protocol for Histone PTMs</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a href=\"/resources/protocols/cutana-cut-and-tag-protocol/\" target=\"blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\">\n <g>\n <path class=\"product-documents__svg-link\" d=\"M130.3,157.06c-0.77-0.76-2-0.76-2.77,0l-28.45,28.45c-13.17,13.17-35.41,14.57-49.95,0\n c-14.57-14.57-13.17-36.78,0-49.95l28.45-28.45c0.76-0.76,0.76-2.01,0-2.77l-9.75-9.75c-0.77-0.76-2-0.76-2.77,0l-28.45,28.45\n c-20.71,20.71-20.71,54.23,0,74.92s54.23,20.71,74.92,0L140,169.52c0.76-0.76,0.76-2.01,0-2.77L130.3,157.06z M193.62,41\n c-20.71-20.71-54.23-20.71-74.92,0L90.22,69.45c-0.76,0.77-0.76,2,0,2.77l9.72,9.72c0.76,0.76,2.01,0.76,2.77,0l28.45-28.45\n c13.17-13.17,35.41-14.57,49.95,0c14.57,14.57,13.17,36.78,0,49.95l-28.45,28.45c-0.76,0.77-0.76,2,0,2.77l9.75,9.75\n c0.76,0.76,2.01,0.76,2.77,0l28.45-28.45C214.31,95.23,214.31,61.71,193.62,41L193.62,41z M139.14,85.29c-0.77-0.76-2-0.76-2.77,0\n l-55.46,55.43c-0.76,0.77-0.76,2,0,2.77l9.7,9.7c0.76,0.76,2.01,0.76,2.77,0l55.43-55.43c0.76-0.76,0.76-2.01,0-2.77L139.14,85.29z\n \" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Previous CUTANA<sup>™</sup> CUT&Tag Protocol versions</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Intellectual Property</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n US Pat. No. 10,689,643, 11,306,307, EU Pat. No. 3,688,157, 2,999,784\n and related patents and pending applications\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 id=\"bioz\" class=\"sub-title1\">Product References</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <object id=\"wobj-3835-15-1017-q\" type=\"text/html\" data=\"https://www.bioz.com/v_widget_6_0/3835/15-1017/?ex=1\"\n style=\"width: 100%; height: 193px\"></object>\n <div id=\"bioz-w-pb-3835-15-1017-q-div\" style=\"width: 100%\">\n <a id=\"bioz-w-pb-3835-15-1017-q\" style=\"font-size: 12px; text-decoration: none; color: #4698cb\"\n href=\"https://www.bioz.com/\" target=\"_blank\"><img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \" />\n Powered by Bioz</a>\n <a style=\"\n font-size: 12px;\n text-decoration: none;\n float: right;\n color: transparent;\n \" href=\"https://www.bioz.com/result/15-1017/product/EpiCypher/?cn=14-1048\" target=\"_blank\">\n See more details on Bioz</a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj('s', [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n '15-1017',\n '15-1017'\n );\n });\n</script>\n\n<style>\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* //////////// */\n\n /* BIOZ */\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n span.bioz-w-parent-hover {\n font-size: 15px;\n }\n\n td .bioz-w-tooltipx {\n padding-top: 0 !important;\n }\n\n /* .bioz-w-parent-hover:hover {\n text-decoration: underline !important;\n } */\n\n /* /////////// */\n\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n .bioz-w-header td {\n padding: 0;\n }\n\n .epicypher-table table {\n margin-right: auto;\n margin-left: auto;\n width: 100%;\n border-top: 1px solid lightgray;\n }\n\n .epicypher-table th {\n color: #3b3a48;\n }\n\n .epicypher-table td {\n color: #3b3a48;\n font-size: 0.9rem;\n }\n\n .epicypher-table td a {\n text-decoration: underline;\n }\n\n .epicypher-table td,\n th {\n border: 0.5px solid lightgray;\n text-align: left;\n padding: 8px;\n /* white-space: nowrap; */\n }\n\n .epicypher-table tr:nth-child(even) {\n background-color: #f9f9f9;\n }\n\n .epicypher-table td:first-child {\n border-left: 1px solid lightgray;\n }\n\n /* Tablet */\n\n @media only screen and (max-width: 1024px) {\n\n /* Force table to not be like tables anymore */\n .epicypher-table th {\n display: none !important;\n }\n\n /* BIOZ */\n td.bioz-w-tooltipx::before {\n display: none;\n }\n\n /* Hide table headers (but not display: none;, for accessibility) */\n .epicypher-table thead tr {\n position: absolute;\n top: -9999px;\n left: -9999px;\n }\n\n .epicypher-table tr {\n border: 1px solid #ccc;\n }\n\n .epicypher-table td {\n /* Behave like a \"row\" */\n /* border: none; */\n border-bottom: 1px solid #eee;\n position: relative;\n padding-left: 30%;\n white-space: inherit;\n }\n\n .epicypher-table td:before {\n /* Now like a table header */\n position: absolute;\n /* Top/left values mimic padding */\n top: 6px;\n left: 6px;\n width: 45%;\n padding-right: 10px;\n white-space: nowrap;\n font-weight: 700;\n }\n\n /*\n Label the data\n */\n .epicypher-table td:nth-of-type(1):before {\n content: 'Item';\n }\n\n td:nth-of-type(2):before {\n content: 'Cat. No.';\n }\n }\n\n @media screen and (max-width: 767px) {\n .custom-title-description {\n font-size: 19px !important;\n }\n\n .section-title {\n font-size: 19px !important;\n padding-left: 1rem;\n }\n }\n</style>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$795.00","value":795,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"CUTANA™ pAG-Tn5 for CUT&Tag","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[{"id":1210,"type":"Configurable_PickList_Set","display_name":"Select Pack Size:","required":true,"condition":true,"state":"variant_option","values":[{"label":"50 Reactions","id":161,"data":"50 Reactions","selected":true},{"label":"250 Reactions","id":162,"data":"250 Reactions","selected":false}],"partial":"set-radio"}],"related_products":[{"id":934,"sku":"15-1026","name":"CUTANA™ Fluorescent pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-assays/cutana-fluorescent-pag-tn5-for-cut-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays","Other/IDEA Toolbox"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 192 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/934/1048/toolbox-icon__95574.1661116173.png?c=2","alt":"CUTANA™ Fluorescent pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/934/1048/toolbox-icon__95574.1661116173.png?c=2","alt":"CUTANA™ Fluorescent pAG-Tn5 for CUT&Tag"}],"date_added":"12th Aug 2022","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1152,"name":"Pack Size","value":"125 µL"},{"id":1153,"name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=934","price":{"without_tax":{"currency":"USD","formatted":"$745.00","value":745},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=934"},{"id":766,"sku":"15-1025","name":"CUTANA™ Uncharged pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-assays/cutana-uncharged-pag-tn5-for-cut-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays","Other/IDEA Toolbox"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 157 kDa (dimer)\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/766/1065/toolbox-icon__65890.1661116229__53021.1661977262.png?c=2","alt":"CUTANA™ Uncharged pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/766/1065/toolbox-icon__65890.1661116229__53021.1661977262.png?c=2","alt":"CUTANA™ Uncharged pAG-Tn5 for CUT&Tag"}],"date_added":"14th Jul 2020","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1206,"name":"Pack Size","value":"20 µL"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=766","price":{"without_tax":{"currency":"USD","formatted":"$645.00","value":645},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=766"},{"id":1160,"sku":"15-1028","name":"CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-assays/cutana-uncharged-6xhis-pag-tn5-for-cut-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays","Other/IDEA Toolbox"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 161,401.8 Da (dimer)\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1160/1233/toolbox-icon__65890.1661116229__13690.1718896281.png?c=2","alt":"CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1160/1233/toolbox-icon__65890.1661116229__13690.1718896281.png?c=2","alt":"CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag"}],"date_added":"20th Jun 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1326,"name":"Pack Size","value":"75 µL"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=1160","price":{"without_tax":{"currency":"USD","formatted":"$645.00","value":645},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=1160"},{"id":986,"sku":null,"name":"CUTANA™ CUT&Tag Kit","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"}],"date_added":"8th Mar 2023","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1219,"name":"Pack Size","value":"48 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$2,695.00","value":2695},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=986"},{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Nuclease\n \n \n Mol Wgt: 43.7 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"}],"date_added":"12th Aug 2019","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1174,"name":"Internal Comment","value":"Excess in bottom of Venom"},{"id":1175,"name":"Internal Comment","value":"Bulk in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$1,295.00","value":1295},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=694"},{"id":875,"sku":"13-2010","name":"HA Tag CUTANA™ CUT&RUN Antibody","url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-compatible-antibodies/ha-tag-cutana-cut-and-run-antibody","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Polyclonal\n \n \n Host: Rabbit\n \n \n Applications: CUT&RUN, WB","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/875/937/ha-tag-cutana-cutandrun-antibody__43540.1645734529.jpg?c=2","alt":"HA Tag CUTANA CUTandRUN Antibody"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/875/937/ha-tag-cutana-cutandrun-antibody__43540.1645734529.jpg?c=2","alt":"HA Tag CUTANA CUTandRUN Antibody"}],"date_added":"21st Jul 2021","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":909,"name":"Pack Size","value":"100 µg"}],"num_reviews":null,"weight":{"formatted":"0.00 LBS","value":0},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=875","price":{"without_tax":{"currency":"USD","formatted":"$495.00","value":495},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=875"},{"id":1136,"sku":"13-2031","name":"DYKDDDDK Tag CUTANA™ 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Description
Available in 50 and 250 Reaction pack sizes. CUTANA pAG-Tn5 is the key reagent for performing efficient mapping of chromatin features in Cleavage Under Targets and Tagmentation (CUT&Tag) [Kaya-Okur et al., 2019]. CUT&Tag offers significant improvements in signal to noise at reduced cell inputs and sequencing depth compared to ChIP-seq. As a fusion of Proteins A and G to a highly active E. coli transposase mutant (Tn5), CUTANA pAG-Tn5 is compatible with target antibodies from a broad spectrum of host species. It also comes without an epitope tag, making it suitable for tag-mediated CUT&Tag (e.g. FLAG, HA, TY1, V5, etc.). This product is highly purified to remove contaminating E. coli DNA, which can be tagmented during storage and complicate analysis at low cell numbers. For superior normalization as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins are recommended. The Tn5 comes charged with mosaic adapters and is ready to be used in CUT&Tag.
Validation Data
Figure 1: Protein gel data
CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE
and stained with Coomassie blue. The migration and molecular
weight of the protein standards are indicated. Uncharged
pAG-Tn5 monomer is 78.5 kDa, however once charged with DNA,
Tn5 dimerizes to a final complex weight of 191 kDa.
Figure 2: Size distribution of released
chromatin
CUT&Tag was performed as described above. Recovered DNA was
directly PCR amplified to produce sequence-ready libraries.
Agilent TapeStation traces for libraries derived from
negative control Rabbit IgG (top) and H3K27me3 (bottom) are
shown. Excised DNA is highly enriched for mononucleosomes
(peak at ~300 bp reflects ~150 bp insert size).
Figure 3: CUT&Tag data
CUT&Tag was performed as described above. Representative
sequencing tracks obtained using CUTANA pAG-Tn5 show a 232
kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced
clear peaks with genomic distribution profiles consistent
with the known biological functions of H3K4me3 and H3K27me3
as well as minimal background in the IgG negative control.
CUT&Tag methods
CUT&Tag was performed on 100k K562 cells with 0.5 µg of
either IgG (EpiCypher
13-0042),
H3K4me3 (EpiCypher
13-0041),
or H3K27me3 (ThermoFisher
MA5-11198) antibodies using CUTANA™ pAG-Tn5 (1:20 dilution)
following the EpiCypher Direct-to-PCR CUT&Tag
protocol. Libraries were run on an
Illumina NextSeq2000 with paired-end sequencing (2x50 bp).
Sample sequencing depth was 0.3 million reads (IgG), 1.7
million reads (H3K4me3), and 3.8 million reads (H3K27me3).
Data were aligned to the hg19 genome using Bowtie2. Data
were filtered to remove duplicates, multi-aligned reads, and
ENCODE DAC Exclusion List regions.
Recommended Accessory Products
Item | Cat. No. |
---|---|
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401 |
SNAP-CUTANA™ K-MetStat Panel | 19-1002 |
Histone H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and ChIP | 13-0041 |
CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody | 13-0042 |
Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag | 13-0047 |
Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag | 13-0048 |
CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag | 15-1018 |
Magnetic Separation Rack, 0.2 mL Tubes | 10-0008 |
Magnetic Separation Rack, 1.5 mL Tubes | 10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
Technical Information
Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’
Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’
Tn5ME-rev: 5’-[phos]CTGTCTCTTATACACATCT-3’
Application Notes
Add 2.5 µL of the supplied enzyme to a 50 µL CUT&Tag reaction (20X dilution). For detailed applications and uses of this product, please see our CUT&Tag protocol.
Documents & Resources
Intellectual Property
US Pat. No. 10,689,643, 11,306,307, EU Pat. No. 3,688,157, 2,999,784 and related patents and pending applications