CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":755,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"CUTANA™ CUT&RUN 8-strip 0.2mL tubes for ChIC / CUT&RUN Assays","category":["Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/755/733/Screen_Shot_2020-02-12_at_11.01.55_AM__47778.1592491202.png?c=2","alt":"CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=755","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"720","name":"Pack size","value":"120 strips"}],"sku":"10-0009","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n CUTANA™ CUT&RUN 8-strip 0.2 mL tubes and attached flat caps are made\n from high quality resins without lubricants or additives for\n reproducible results. The tubes are autoclavable and supplied RNase,\n DNase, and DNA free.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Product Images</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/genomics/10-0009-front.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"10-0009-front\"\n src=\"/content/images/products/genomics/10-0009-front.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: CUTANA™ CUT&RUN 8-strip 0.2mL tubes</strong\n ><br />\n 8-strip 0.2 mL tubes enable multichannel pipetting for\n improved sample handling and increased experimental\n throughput in CUT&RUN applications. The tubes are pictured\n with CUTANA™ Concanavalin A (ConA)-Conjugated Paramagnetic\n Beads (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads\"\n >21-1401</a\n >), which are used for immobilizing cells or nuclei during\n the CUT&RUN procedure. CUTANA™ Concanavalin A-Conjugated\n Paramagnetic Beads are not included in purchase of this\n product.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/genomics/10-0009-quarter-view.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"10-0009-quarter-view\"\n src=\"/content/images/products/genomics/10-0009-quarter-view.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: CUTANA™ CUT&RUN 8-strip 0.2mL tubes</strong\n ><br />\n 8-strip 0.2 mL tubes are compatible with the Magnetic\n Separation Rack (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\"\n >10-0008</a\n >) for use during all bead separation steps in the CUT&RUN\n procedure, including sample immobilization with ConA beads\n and library preparation. Magnetic Separation Rack is not\n included in purchase of this product.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"10-0009-front\"\n src=\"/content/images/products/genomics/10-0009-front.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"10-0009-quarter-view\"\n src=\"/content/images/products/genomics/10-0009-quarter-view.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Features</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <ul>\n <li>Clear tubes supplied in boxes of 120 strips</li>\n <li>Compatible for 960 CUTANA™ CUT&RUN reactions</li>\n </ul>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n Suitable for cell-based sorting and molecular biology applications,\n including CUTANA™\n <a href=\"https://www.epicypher.com/technologies/cutana/cut-and-run\"\n >CUT&RUN</a\n >\n and\n <a href=\"https://www.epicypher.com/technologies/cutana/cut-and-tag\"\n >CUT&Tag</a\n >\n technologies. Also needed for the\n <a\n href=\"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n >CUT&RUN Library Prep Kit</a\n >.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/10-0009.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$195.00","value":195,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"CUTANA™ CUT&RUN 8-strip 0.2mL tubes","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":986,"sku":null,"name":"CUTANA™ CUT&Tag Kit","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"}],"date_added":"8th Mar 2023","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1219,"name":"Pack Size","value":"48 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$2,695.00","value":2695},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=986"},{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Nuclease\n \n \n Mol Wgt: 43.7 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"}],"date_added":"12th Aug 2019","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1174,"name":"Internal Comment","value":"Excess in bottom of Venom"},{"id":1175,"name":"Internal Comment","value":"Bulk in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$1,295.00","value":1295},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=694"},{"id":764,"sku":null,"name":"CUTANA™ pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n Type: Transposase\n \n \n Mol Wgt: 191 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"}],"date_added":"17th Jun 2020","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1005,"name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$795.00","value":795},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$795.00","value":795},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$2,995.00","value":2995},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=764"}],"shipping_messages":[],"rating":0,"meta_keywords":"CUTANA™ CUT&RUN 8-strip 0.2mL tubes, pcr tubes, 10-0006","show_quantity_input":1,"title":"CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/755/733/Screen_Shot_2020-02-12_at_11.01.55_AM__47778.1592491202.png?c=2","alt":"CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes"}]} Pack size: 120 strips
Description
CUTANA™ Bead Activation Buffer is used to prepare Concanavalin A (ConA) conjugated paramagnetic beads for CUT&RUN and CUT&Tag. Activated ConA beads are used in both CUT&RUN and CUT&Tag workflows to immobilize cells or nuclei.
Validation Data
(Click to enlarge)
ConA beads were washed with CUTANA™ Bead Activation Buffer and loaded with K562 cells as described in the CUTANA™ CUT&RUN and CUT&Tag protocols (www.epicypher.com/protocols). Trypan Blue staining shows cells before (panels A and B) and after (C and D) bead binding. After incubation with activated beads, the supernatant contains few unbound cells (C), demonstrating efficient binding. Permeabilized cells bound to activated beads are observed in the cell-bead slurry (D).
Recommended Companion Products
ITEM | CAT. NO. |
---|---|
CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
CUTANA™ CUT&Tag Kit | 14-1102/14-1103 |
CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
CUTANA™ pAG-Tn5 for CUT&Tag | 15-1017/15-1117 |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
CUTANA™ E. coli Spike-in DNA | 18-1401 |
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Technical Information
Documents & Resources
Description
CUTANA™ Stop Buffer is used in CUT&RUN to terminate pAG-MNase activity and prevent over-digestion of released DNA fragments. The Stop Buffer is added following pAG-MNase incubation, and halts enzyme activity by chelating free calcium ions.
Validation Data
(Click to enlarge)
Recommended Companion Products
ITEM | CAT. NO. |
---|---|
CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
CUTANA™ E. coli Spike-in DNA | 18-1401 |
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Technical Information
Documents & Resources
Description
Digitonin is a nonionic detergent used in CUTANA™ CUT&RUN and CUT&Tag genomic mapping assays to permeabilize cells, facilitating antibody and enzyme access to chromatin. Digitonin also helps prevent clumping of Concanavalin A (ConA) magnetic beads and film formation on tubes, which minimizes sample loss. For this reason, Digitonin is recommended in assay buffers for both cell and nuclei samples. Refer to the appropriate kit manual or do-it-yourself (DIY) protocol for guidance on which assay buffers require Digitonin.
Validation Data
(Click to enlarge)
Recommended Companion Products
ITEM | CAT. NO. |
---|---|
CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
CUTANA™ CUT&Tag Kit | 14-1102/14-1103 |
CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
CUTANA™ pAG-Tn5 for CUT&Tag | 15-1017/15-1117 |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
CUTANA™ E. coli Spike-in DNA | 18-1401 |
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Technical Information
See www.epicypher.com/protocols for detailed protocols. Visit support.epicypher.com for guidance on optimizing digitonin concentration.
Documents & Resources
Description
CUTANA™ CUT&RUN 8-strip 0.2 mL tubes and attached flat caps are made from high quality resins without lubricants or additives for reproducible results. The tubes are autoclavable and supplied RNase, DNase, and DNA free.
Product Images
Figure 1: CUTANA™ CUT&RUN 8-strip 0.2mL tubes
8-strip 0.2 mL tubes enable multichannel pipetting for
improved sample handling and increased experimental
throughput in CUT&RUN applications. The tubes are pictured
with CUTANA™ Concanavalin A (ConA)-Conjugated Paramagnetic
Beads (EpiCypher
21-1401), which are used for immobilizing cells or nuclei during
the CUT&RUN procedure. CUTANA™ Concanavalin A-Conjugated
Paramagnetic Beads are not included in purchase of this
product.
Figure 2: CUTANA™ CUT&RUN 8-strip 0.2mL tubes
8-strip 0.2 mL tubes are compatible with the Magnetic
Separation Rack (EpiCypher
10-0008) for use during all bead separation steps in the CUT&RUN
procedure, including sample immobilization with ConA beads
and library preparation. Magnetic Separation Rack is not
included in purchase of this product.
Features
- Clear tubes supplied in boxes of 120 strips
- Compatible for 960 CUTANA™ CUT&RUN reactions
Application Notes
Suitable for cell-based sorting and molecular biology applications, including CUTANA™ CUT&RUN and CUT&Tag technologies. Also needed for the CUT&RUN Library Prep Kit.