

SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca4-chromatin-remodeling-enzyme-human-brg1","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":651,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Enzymatically active full-length SMARCA4 (human BRG1) for use in HTS drug discovery assays and chromatin binding experiments. ","category":["Recombinant Proteins/Modifying Enzymes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/651/616/Untitled__68998.1556128740.png?c=2","alt":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=651","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1140","name":"Pack size","value":"100 reactions"},{"id":"1141","name":"Internal Comment","value":"21-1014 is sold with this item."},{"id":"1142","name":"Internal Comment","value":"51 units of R&D Lot 7 are in HQ"},{"id":"1143","name":"Internal Comment","value":"Bulk in Protein Box in LG, Lot 7"}],"sku":"15-1014","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong>SF9 cells\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag: </strong>6xHis-FLAG\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Mol Wgt: </strong>186.6 kDa\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\">\n <p>\n Full length recombinant human SMARCA4 Remodeling Enzyme. SMARCA4 is\n a core ATPase subunit of the ATP-dependent chromatin remodeling\n complex SWI/SNF that mobilizes nucleosomes and plays key roles in\n gene transcription regulation, development, genome replication and\n repair [1,2]. Aberrant activity of SWI/SNF subunits is linked with\n more than 20% of human cancers and multiple developmental disorders,\n making SMARCA4 an emerging candidate therapeutic target [3,4].\n SMARCA4 can be used in conjunction with EpiDyne<sup>®</sup>-FRET\n Remodeling Assay Substrate (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\"\n >16-4201</a\n >) in HTS-compatible drug discovery studies.\n </p>\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a\n target=\"_blank\"\n style=\"color: #fff\"\n href=\"/resources/protocols/epidyne-guide\"\n >Want to learn more? Check out our EpiDyne® Guide >>\n </a>\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1014-chromatin-remodeling-assay.png\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"15-1014-chromatin-remodeling-assay\"\n src=\"/content/images/products/proteins/15-1014-chromatin-remodeling-assay.png\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: ATP-dependent chromatin remodeling assay </strong\n ><br />EpiDyne<sup>®</sup>-FRET Chromatin Remodeling\n Substrate (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\"\n >16-4201</a\n >; 20nM) incubated with SMARCA4 Remodeling Enzyme\n (concentrations indicated) in 1x SMARCA Remodeling Assay\n Buffer. Curves denote FRET efficiency/chromatin remodeling.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1014-protein-gel.png\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"15-1014-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1014-protein-gel.png\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein gel data </strong><br />\n SMARCA4 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel\n and stained with Coomassie blue. The migration and molecular\n weight of the protein standards are indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"15-1014-chromatin-remodeling-assay\"\n src=\"/content/images/products/proteins/15-1014-chromatin-remodeling-assay.png\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"15-1014-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1014-protein-gel.png\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 350 mM NaCl, 1 mM\n DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL\n insulin.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months -80°C from date of receipt. For best\n results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This product is sufficient to perform 100 remodeling reactions using\n EpiDyne® -FRET substrate (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\"\n >16-4201</a\n >). A single reaction is defined as 10 µL containing a lot-specific\n concentration of SMARCA4 (see Technical Datasheet), 20 nM EpiDyne®\n -FRET, & 1 mM rATP with remodeling to completion in <30 minutes.\n Remodeling readout can be observed in the change of Cy3/Cy5 ratio\n over time in a black 384-well assay plate on a compatible\n fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is\n included (EpiCypher\n <a\n href=\"/products/recombinant-proteins/modifying-enzymes/5x-smarca-remodeling-assay-buffer\"\n >21-0014</a\n >). For more information, see the\n <a href=\"/resources/technical-notes/\"\n >EpiDyne<sup>®</sup>-FRET Technote</a\n >\n or contact\n <a href=\"mailto:techsupport@epicypher.com\"\n >techsupport@epicypher.com</a\n >.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">P51532-1</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Gene Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">SMARCA4</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Protein Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Transcription activator BRG1\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Amino Acids</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 1-1647 with a C-terminal 6His-FLAG\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Alternate Names</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n BAF190A, BRG1, SNF2B, SNF2L4\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Clapier & Carins <em>Annu. Rev. Biochem.</em> (2009). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/19355820/\"\n title=\"The biology of chromatin remodeling complexes\"\n target=\"new\">\n 19355820</a\n ><br />\n [2] Sundaramoorthy & Owen-Hughes <em>F1000Research</em> (2020). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/32864100/\"\n title=\"Chromatin remodelling comes into focus\"\n target=\"new\">\n 32864100</a\n ><br />\n [3] Kadoch & Crabtree <em>Sci. Adv.</em> (2015). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/26601204/\"\n title=\"Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics\"\n target=\"new\">\n 26601204</a\n ><br />\n [4] Cenik & Shilatifard <em>Cells</em> (2022). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/32958894/\"\n title=\"COMPASS and SWI/SNF complexes in development and disease\"\n target=\"new\">\n 32958894</a\n ><br />\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/15-1014.pdf\"\n target=\"blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1) ","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":922,"sku":"15-1024","name":"SMARCA5 Chromatin Remodeling Enzyme (Human SNF2H)","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-sup-sup-modifying-enzymes/smarca5-chromatin-remodeling-enzyme-human-snf2h","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/922/1026/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.1280.1280.jpg_c_2%26_gl_1_1rzfkza__ga_MTc4NjU1MDYxMi4xNjU1MzE1MzYx__ga_WS2VZYPC6G_MTY1NTk5NzEwNy4yMy4xLjE2NTU5OTcxMzEuMzY__08994.1655997178.jpg?c=2","alt":"SMARCA5 Chromatin Remodeling Enzyme (Human 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(Human BRM)","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca2-chromatin-remodeling-enzyme-human-brm","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2","alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2","alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM"}],"date_added":"15th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1154,"name":"Pack Size","value":"100 Reactions"},{"id":1155,"name":"Internal Comment","value":"extra in top right of Psylocke"},{"id":1156,"name":"Internal Comment","value":"bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=650","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=650"},{"id":551,"sku":"15-1013","name":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","availability":"","rating":null,"brand":{"name":null},"category":[],"summary":"\n \n 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- Species: Human
- Source: SF9 cells
- Epitope Tag: 6xHis-FLAG
- Mol Wgt: 186.6 kDa
Description
Full length recombinant human SMARCA4 Remodeling Enzyme. SMARCA4 is a core ATPase subunit of the ATP-dependent chromatin remodeling complex SWI/SNF that mobilizes nucleosomes and plays key roles in gene transcription regulation, development, genome replication and repair [1,2]. Aberrant activity of SWI/SNF subunits is linked with more than 20% of human cancers and multiple developmental disorders, making SMARCA4 an emerging candidate therapeutic target [3,4]. SMARCA4 can be used in conjunction with EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201) in HTS-compatible drug discovery studies.
Validation Data
Figure 1: ATP-dependent chromatin remodeling assay
EpiDyne®-FRET Chromatin Remodeling
Substrate (EpiCypher
16-4201; 20nM) incubated with SMARCA4 Remodeling Enzyme
(concentrations indicated) in 1x SMARCA Remodeling Assay
Buffer. Curves denote FRET efficiency/chromatin remodeling.
Figure 2: Protein gel data
SMARCA4 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel
and stained with Coomassie blue. The migration and molecular
weight of the protein standards are indicated.
Technical Information
Application Notes
This product is sufficient to perform 100 remodeling reactions using EpiDyne® -FRET substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing a lot-specific concentration of SMARCA4 (see Technical Datasheet), 20 nM EpiDyne® -FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014). For more information, see the EpiDyne®-FRET Technote or contact techsupport@epicypher.com.