SKU: 16-4201
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":386,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"EpiDyne FRET-based Chromatin Remodeling Assay Substrate for inhibitor screening and HTS assays","category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/386/276/2_EpiDyne__20008.1569012494.png?c=2","alt":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=386","shipping":{"calculated":true},"num_reviews":0,"weight":"0.00 LBS","custom_fields":[{"id":"1138","name":"Pack Size","value":"50 µg"},{"id":"1139","name":"Internal Comment","value":"Bulk in Bulk Nuc Box 2 in Psylocke"}],"sku":"16-4201","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <p>\n This product provides a high throughput fluorescent readout of\n nucleosome remodeling when paired with enzymatically active\n remodeling complexes, such as SMARCA4, SMARCA2, and ACF (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca4-chromatin-remodeling-enzyme-human-brg1\"\n >15-1014</a\n >,\n <a\n href=\"/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca2-chromatin-remodeling-enzyme-human-brm\"\n >15-1015</a\n >,\n <a\n href=\"/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex\"\n >15-1013</a\n >). The EpiDyne<sup>®</sup>-FRET mononucleosome is assembled from\n recombinant human histones expressed in <em>E. coli</em> (two each\n of histones H2A-Cy5, H2B, H3.2 and H4; accession numbers:\n H2A-P04908; H2B-O60814; H3.2-Q71DI3; H4-P62805) wrapped with a 212\n base-pair DNA template bearing a 5’ Cy3, the Widom 601 nucleosome\n positioning element [1], and a DpnII restriction enzyme motif (GATC)\n that is exposed upon remodeling. H2A has a Thr to Cys substitution\n at residue 120, where Cy5 is conjugated. H3.2 has a Cys to Ala\n substitution at residue 110.\n </p>\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a\n target=\"_blank\"\n style=\"color: #fff\"\n href=\"/resources/protocols/epidyne-guide\"\n >Want to learn more? Check out our EpiDyne® Guide >>\n </a>\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"content/images/products/nucleosomes/16-4201-dna-gel-data.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-4201-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-4201-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA Gel Data </strong>\n <br />\n EpiDyne-FRET substrate resolved via native PAGE gel and\n stained with ethidium bromide to visualize DNA.\n <strong>Lane 1</strong>: Free DNA (100 ng).\n <strong>Lane 2</strong>: Intact EpiDyne-FRET nucleosomes\n (400 ng).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-4201-protein-gel-data.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-4201-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-4201-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein Gel Data </strong><br />\n Coomassie stained PAGE gel of proteins in EpiDyne-FRET\n substrate (1 µg) demonstrates the purity of histones in the\n preparation. Sizes of molecular weight markers and positions\n of the core histones (H2A-Cy5, H2B, H3.2, and H4) are\n indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-4201-nucleosome-remodeling-data.jpeg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img loading=\"lazy\"\n alt=\"16-4201-nucleosome-remodeling-data\"\n src=\"/content/images/products/nucleosomes/16-4201-nucleosome-remodeling-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Nucleosome Remodeling Data </strong><br />\n ACF1/ATP-dependent nucleosome remodeling reaction.\n EpiDyne-FRET nucleosomes (15 nM) were incubated with ACF1\n chromatin remodeler (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex\"\n >15-1013</a\n >; indicated concentrations) in the presence or absence of 1\n mM ATP. Upon the addition of ATP, reactions were immediately\n read in an Envision Multilabel plate reader (PerkinElmer).\n Data are presented as the mean of the Cy3/Cy5 ratio (N=2).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"16-4201-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-4201-dna-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"16-4201-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-4201-protein-gel-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"16-4201-nucleosome-remodeling-data\"\n src=\"/content/images/products/nucleosomes/16-4201-nucleosome-remodeling-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p>\n Purified recombinant mononucleosomes, (21.8 µg protein weight,\n 50 µg DNA+protein) in 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM\n EDTA, 2 mM DTT, 20% glycerol. MW = 240,650 Da.\n </p>\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This product is a template for nucleosome remodeling assays using\n Cy3/Cy5 FRET or using the restriction enzyme DpnII to determine\n accessibility of GATC, which is masked in its native configuration\n (prior to remodeling).\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J Mol. Biol.</em> (1998) PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n target=\"_blank\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n >9514715</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-4201.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$595.00","value":595,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"EpiDyne FRET-based Chromatin Remodeling Assay Substrate","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":650,"sku":"15-1015","name":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca2-chromatin-remodeling-enzyme-human-brm","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2","alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2","alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM"}],"date_added":"15th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1154,"name":"Pack Size","value":"100 Reactions"},{"id":1155,"name":"Internal Comment","value":"extra in top right of Psylocke"},{"id":1156,"name":"Internal Comment","value":"bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=650","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=650"},{"id":651,"sku":"15-1014","name":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca4-chromatin-remodeling-enzyme-human-brg1","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/651/616/Untitled__68998.1556128740.png?c=2","alt":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/651/616/Untitled__68998.1556128740.png?c=2","alt":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)"}],"date_added":"15th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1140,"name":"Pack size","value":"100 reactions"},{"id":1141,"name":"Internal Comment","value":"21-1014 is sold with this item."},{"id":1142,"name":"Internal Comment","value":"51 units of R&D Lot 7 are in HQ"},{"id":1143,"name":"Internal Comment","value":"Bulk in Protein Box in LG, Lot 7"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=651","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=651"},{"id":551,"sku":"15-1013","name":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","availability":"","rating":null,"brand":{"name":null},"category":[],"summary":"\n \n Product Discontinued - we recommend SMARCA5 (15","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2","alt":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2","alt":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*"}],"date_added":"24th Jul 2018","pre_order":false,"show_cart_action":false,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":585,"name":"Pack Size","value":"100 reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=551"},{"id":347,"sku":"16-4101","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1","url":"https://www.epicypher.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":"\n \n \n \n Description\n \n \n Mononucleosomes assembled","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/347/803/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__47518.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/347/803/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__47518.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/347/802/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_no_background_RGB_AL_MP__86463.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1"}],"date_added":"1st Mar 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1053,"name":"Pack Size","value":"50 μg"},{"id":1054,"name":"Internal Comment","value":"no more bulk 3/31/22"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=347","price":{"without_tax":{"currency":"USD","formatted":"$565.00","value":565},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=347"},{"id":556,"sku":"18-4201","name":"EpiDyne-FRET Remodeling Assay Substrate 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Pack Size: 50 µg
