SKU: 15-1024
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-sup-sup-modifying-enzymes/smarca5-chromatin-remodeling-enzyme-human-snf2h","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":922,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Enzymatically active full-length SMARCA5 (human SNF2H) for use in HTS drug discovery assays and chromatin binding experiments. ","category":["Recombinant Proteins/Modifying Enzymes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/922/1026/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.1280.1280.jpg_c_2&_gl_1_1rzfkza__ga_MTc4NjU1MDYxMi4xNjU1MzE1MzYx__ga_WS2VZYPC6G_MTY1NTk5NzEwNy4yMy4xLjE2NTU5OTcxMzEuMzY__08994.1655997178.jpg?c=2","alt":"SMARCA5 Chromatin Remodeling Enzyme (Human SNF2H)"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=922","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1109","name":"Pack Size","value":"100 Reactions"}],"sku":"15-1024","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong>SF9 cells\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag: </strong>FLAG-6His\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Mol Wgt: </strong>123.7 kDa\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\">\n <p>\n Full length recombinant human SMARCA5 Remodeling Enzyme. SMARCA5 is\n a core ATPase subunit of the ATP-dependent chromatin remodeling ISWI\n complexes that regulate nucleosome spacing to control DNA\n accessibility [1,2]. SMARCA5 containing complexes exhibit substrate\n preference and distinct remodeling directionalities with different\n regulatory subunits [3]. Mis-regulation of SMARCA5 is linked with\n multiple human cancers including leukemia, breast, lung, and gastric\n cancers [4]. SMARCA5 can be used in conjunction with\n EpiDyne<sup>®</sup>-FRET Remodeling Assay Substrate (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\"\n >16-4201</a\n >) in HTS-compatible drug discovery studies.\n </p>\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a\n target=\"_blank\"\n style=\"color: #fff\"\n href=\"/resources/protocols/epidyne-guide\"\n >Want to learn more? Check out our EpiDyne® Guide >>\n </a>\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1024-chromatin-remodeling-assay.png\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"15-1024-chromatin-remodeling-assay\"\n src=\"/content/images/products/proteins/15-1024-chromatin-remodeling-assay.png\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: ATP-dependent chromatin remodeling assay </strong\n ><br />EpiDyne<sup>®</sup>-FRET Chromatin Remodeling\n Substrate (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\"\n >16-4201</a\n >; 20nM) incubated with SMARCA5 Remodeling Enzyme\n (concentrations indicated) in 1x SMARCA Remodeling Assay\n Buffer. Curves denote FRET efficiency/chromatin remodeling.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/proteins/15-1024-protein-gel-data.jpeg\"\n target=\"blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"15-1024-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1024-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein gel data </strong><br />\n SMARCA5 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel\n and stained with Coomassie blue. The migration and molecular\n weight of the protein standards are indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"15-1024-chromatin-remodeling-assay\"\n src=\"/content/images/products/proteins/15-1024-chromatin-remodeling-assay.png\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"15-1024-protein-gel-data\"\n src=\"/content/images/products/proteins/15-1024-protein-gel-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 200 mM NaCl, 1 mM\n DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL\n insulin.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months -80°C from date of receipt. For best\n results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This product is sufficient to perform 100 remodeling reactions using\n EpiDyne<sup>®</sup>-FRET Substrate (EpiCypher\n <a\n href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\"\n >16-4201</a\n >). A single reaction is defined as 10 µL containing a lot-specific\n concentration of SMARCA5 (see Technical Datasheet), 20 nM\n EpiDyne<sup>®</sup>-FRET, & 1 mM rATP with remodeling to completion\n in <30 minutes. Remodeling readout can be observed in the change\n of Cy3/Cy5 ratio over time in a black 384-well assay plate on a\n compatible fluorescent plate reader. 5x SMARCA Remodeling Assay\n Buffer is included (EpiCypher\n <a\n href=\"/products/recombinant-proteins/modifying-enzymes/5x-smarca-remodeling-assay-buffer\"\n >21-0014</a\n >). For more information, see the\n <a href=\"/resources/technical-notes/\"\n >EpiDyne<sup>®</sup>-FRET Technote</a\n >\n or contact\n <a href=\"mailto:techsupport@epicypher.com\"\n >techsupport@epicypher.com</a\n >.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">O60264-1</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Gene Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">SMARCA5</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Protein Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n SWI/SNF-related matrix-associated actin-dependent regulator of\n chromatin subfamily A member 5\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Amino Acids</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 2-1052 with an N-terminal FLAG-6His\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Alternate Names</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n SNF2H, WCRF135, Sucrose nonfermenting protein 2 homolog\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Clapier & Carins <em>Annu. Rev. Biochem.</em> (2009). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/19355820/\"\n title=\"The biology of chromatin remodeling complexes\"\n target=\"new\">\n 19355820</a\n ><br />\n [2] Sundaramoorthy & Owen-Hughes <em>F1000Research</em> (2020). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/32864100/\"\n title=\"Chromatin remodelling comes into focus\"\n target=\"new\">\n 32864100</a\n ><br />\n [3] Oppikofer et al. <em>EMBO Rep.</em> (2017). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/28801535/\"\n title=\"Expansion of the ISWI chromatin remodeler family with new active complexes\"\n target=\"new\">\n 28801535</a\n ><br />\n [4] Thakur et al. <em>Cells</em> (2022). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/35269430/\"\n title=\"Chromatin Remodeler Smarca5 Is Required for Cancer-Related Processes of Primary Cell Fitness and Immortalization\"\n target=\"new\">\n 35269430</a\n ><br />\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/15-1024.pdf\"\n target=\"blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$405.00","value":405,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"SMARCA5 Chromatin Remodeling Enzyme (Human SNF2H)","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":650,"sku":"15-1015","name":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca2-chromatin-remodeling-enzyme-human-brm","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2","alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/650/953/smarca2-chromatin-remodeling-enzyme-human-brm__84445.1645734545.jpg?c=2","alt":"SMARCA2 Chromatin Remodeling Enzyme Human BRM"}],"date_added":"15th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1154,"name":"Pack Size","value":"100 Reactions"},{"id":1155,"name":"Internal Comment","value":"extra in top right of Psylocke"},{"id":1156,"name":"Internal Comment","value":"bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=650","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=650"},{"id":651,"sku":"15-1014","name":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca4-chromatin-remodeling-enzyme-human-brg1","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes ","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes","Recombinant Proteins/Modifying Enzymes"],"summary":"\n \n \n Species: Human\n \n \n Source: SF9 cells\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/651/616/Untitled__68998.1556128740.png?c=2","alt":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/651/616/Untitled__68998.1556128740.png?c=2","alt":"SMARCA4 Chromatin Remodeling Enzyme (Human BRG1)"}],"date_added":"15th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1140,"name":"Pack size","value":"100 reactions"},{"id":1141,"name":"Internal Comment","value":"21-1014 is sold with this item."},{"id":1142,"name":"Internal Comment","value":"51 units of R&D Lot 7 are in HQ"},{"id":1143,"name":"Internal Comment","value":"Bulk in Protein Box in LG, Lot 7"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=651","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=651"},{"id":551,"sku":"15-1013","name":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","availability":"","rating":null,"brand":{"name":null},"category":[],"summary":"\n \n Product Discontinued - we recommend SMARCA5 (15","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2","alt":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2","alt":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*"}],"date_added":"24th Jul 2018","pre_order":false,"show_cart_action":false,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":585,"name":"Pack Size","value":"100 reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 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Pack Size: 100 Reactions
