CUTANA™ 5% Digitonin
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-digitonin","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":1363,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Digitonin is a nonionic detergent used in CUTANA™ CUT&RUN and CUT&Tag genomic mapping assays to permeabilize cells, facilitating antibody and enzyme access to chromatin.","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1363/1255/Picture1__22337.1737801428.jpg?c=2","alt":"CUTANA™ 5% Digitonin"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=1363","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1341","name":"Pack Size","value":"1 mL"}],"sku":"21-1004","description":"<!-- <div class=\"product-general-info\">\n <ul style=\"display: none\" class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"display: none\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div id=\"w-s-3835-21-1026\" style=\"\n width: max-content;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-21-1026-div\">\n <a id=\"bioz-w-pb-21-1026\" style=\"font-size: 12px; color: transparent\" href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n </a>\n </div>\n </a>\n </li>\n </ul>\n</div> -->\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n Digitonin is a nonionic detergent used in CUTANA™ CUT&RUN and CUT&Tag genomic mapping assays to permeabilize\n cells, facilitating antibody and enzyme access to chromatin. Digitonin also helps prevent clumping of\n Concanavalin A (ConA) magnetic beads and film formation on tubes, which minimizes sample loss. For this\n reason, Digitonin is recommended in assay buffers for both cell and nuclei samples. Refer to the appropriate\n kit manual or do-it-yourself (DIY) protocol for guidance on which assay buffers require Digitonin.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/kits/21-1004-cell-permeabilization.jpg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"21-1004-cell-permeabilization.jpg\"\n src=\"/content/images/products/kits/21-1004-cell-permeabilization.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: Cell permeabilization with\n Digitonin</strong><br />\n The minimum amount of CUTANA™ 5% Digitonin needed to permeabilize K562 cells was determined by\n serial dilution of 5% Digitonin in Wash Buffer. Trypan Blue staining was used to evaluate\n permeability with an automated cell counter (live/dead cell viability analysis). Green cells (Trypan\n negative) are intact, whereas permeabilized/dead cells (Trypan positive) are red. Values (bottom\n right of each panel) indicate percent of dead/permeabilized cells\n </span>\n </p>\n </div>\n\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/kits/21-1004-quantification-of-cell-permeabilization.jpg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"21-1004-quantification-of-cell-permeabilization.jpg\"\n src=\"/content/images/products/kits/21-1004-quantification-of-cell-permeabilization.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: Quantification of cell permeabilization with Digitonin</strong><br />\n Quantification of live/dead cells as shown in Figure 1. 0.01% was selected as the optimal concentration of Digitonin to efficiently permeabilize cells.\n </span>\n </p>\n </div>\n\n <!-- <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/14_1001_distribution_analysis.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"14_1001_distribution_analysis\"\n src=\"/content/images/products/nucleosomes/14_1001_distribution_analysis.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: CUT&RUN DNA Fragment Size Distribution\n Analysis</strong\n ><br />\n CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit\n (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >) starting with 500k K562 cells. Five nanograms of CUT&RUN\n output DNA from reactions utilizing IgG (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >), H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >), H3K27me3 (ABclonal A16199), and CTCF (EpiCypher\n <a\n href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ctcf-cutana-cut-and-run-antibody\"\n >13-2014</a\n >) antibodies were prepared for paired-end Illumina<sup\n >®</sup\n >\n sequencing with the CUTANA CUT&RUN Library Prep Kit. Library\n DNA was analyzed by Agilent TapeStation<sup>®</sup>, which\n confirmed that mononucleosomes were predominantly enriched\n in CUT&RUN (~300 bp peaks represent 150 bp nucleosomes +\n sequencing adapters). Peaks at ~380 bp correspond to the\n SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/14_1001_target_compatibility.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n loading=\"lazy\"\n alt=\"14_1001_target_compatibility\"\n src=\"/content/images/products/nucleosomes/14_1001_target_compatibility.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Representative gene browser tracks</strong\n ><br />\n\n CUT&RUN sequencing libraries described in Figure 2 were\n sequenced on an Illumina<sup>®</sup> NextSeq 2000 with a P3\n cartridge (paired-end 2x50 cycle). Data were aligned to the\n hg19 genome using Bowtie2. Data were filtered to remove\n duplicates, multi-aligned reads, and blacklist regions. A\n representative 640 kb window at the SEPTIN5 gene is shown\n for three replicates (\"Rep\") of IgG and H3K4me3 antibodies,\n as well as individual tracks for H3K27me3 and the\n transcription factor CTCF, demonstrating the robustness and\n reproducibility of the workflow with a variety of targets.\n Sequencing libraries prepared with the CUTANA CUT&RUN\n Library Prep kit produced the expected genomic distribution\n for each target. Sample sequencing depth was as follows\n (millions of reads): IgG Rep 1 (20.1), IgG Rep 2 (16.6), IgG\n Rep 3 (16.1), H3K4me3 Rep 1 (7.3), H3K4me3 Rep 2 (17.2),\n H3K4me3 Rep 3 (13.8), H3K27me3 (13.4), CTCF (16.2). Images\n were generated using the Integrative Genomics Viewer (IGV,\n Broad Institute).\n </span>\n </p>\n </div> -->\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\" alt=\"21-1004-cell-permeabilization.jpg\"\n src=\"/content/images/products/kits/21-1004-cell-permeabilization.jpg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" />\n <img loading=\"lazy\" alt=\"21-1004-quantification-of-cell-permeabilization\"\n src=\"/content/images/products/kits/21-1004-quantification-of-cell-permeabilization.jpg\" class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Accessory Products</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA<sup>™</sup> ChIC/CUT&RUN Kit </td>\n <td>\n <a href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-kit\"\n style=\"text-decoration: none;\">\n 14-1048/14-1048-24\n </a>\n </td>\n </tr>\n\n <tr>\n <td>CUTANA<sup>™</sup> CUT&RUN Library Prep Kit </td>\n <td>\n <a href=\"/products/epigenetics-kits-and-reagents/cutana-cut-run-library-prep-kit\"\n style=\"text-decoration: none;\">\n 14-1001/14-1002\n </a>\n </td>\n </tr>\n\n <tr>\n <td>CUTANA<sup>™</sup> CUT&Tag Kit </td>\n <td>\n <a style=\"text-decoration: none;\"\n href=\"/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit\">\n 14-1102/14-1103\n </a>\n </td>\n </tr>\n\n <tr>\n <td>CUTANA<sup>™</sup> pAG-MNase for ChIC/CUT&RUN </td>\n <td>\n <a style=\"text-decoration: none;\"\n href=\"/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows\">\n 15-1016/15-1116\n </a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUTANA<sup>™</sup> pAG-Tn5 for CUT&Tag</td>\n <td>\n <a style=\"text-decoration: none;\"\n href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\">\n 15-1017/15-1117\n </a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUTANA<sup>™</sup> Concanavalin A Conjugated Paramagnetic Beads</td>\n <td>\n <a style=\"text-decoration: none;\"\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-concanavalin-a-conjugated-paramagnetic-beads\">\n 21-1401/21-1411\n </a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUTANA<sup>™</sup> Nuclei Extraction Buffer</td>\n <td>\n <a style=\"text-decoration: none;\"\n href=\"/products/epigenetics-kits-and-reagents/cutana-nuclei-extraction-buffer\">\n 21-1026\n </a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUTANA<sup>™</sup> <em>E. coli</em> Spike-in DNA</td>\n <td>\n <a style=\"text-decoration: none;\"\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-e-coli-spike-in-dna\">\n 18-1401\n </a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes</td>\n <td>\n <a style=\"text-decoration: none;\"\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\">\n 10-0008</a>/<a style=\"text-decoration: none;\"\n href=\"/products/epigenetics-reagents-and-assays/magnetic-separation-rack-1-5-ml-tubes\">10-0012</a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUTANA<sup>™</sup> CUT&RUN 8-strip 0.2 mL Tubes</td>\n <td>\n <a style=\"text-decoration: none;\"\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes\">\n 10-0009\n </a>\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 2 years at -20°C from date of receipt.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 5% (w/v) digitonin in DMSO.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for Use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n CUTANA™ 5% Digitonin can be used to prepare buffers for use in CUT&RUN or CUT&Tag. If using intact cells, the concentration of digitonin should be optimized for efficient cell permeabilization. To avoid digitonin precipitation and cell lysis, use the minimal concentration of digitonin required for efficient cell permeabilization. EpiCypher has found 0.01% Digitonin to be the optimal concentration for most cell types.\n <br>\n See <a href=\"/protocols\" target=\"_blank\">www.epicypher.com/protocols</a> for detailed protocols. Visit <a href=\"https://support.epicypher.com\" target=\"_blank\">support.epicypher.com</a> for guidance on optimizing digitonin concentration.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n\n\n <!-- <h3\n style=\"color: #4698cb; font-size: 24px; border-bottom: none\"\n class=\"custom-title-description\">\n Documents & Resources\n </h3> -->\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <!-- <h3 style=\"color: #4698cb; margin-bottom: 0\">Current Lot</h3> -->\n <h3 class=\"sub-title1\">Documents & Resources </h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/21-1004.pdf\" target=\"_blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n\n </div>\n</div>\n\n<!-- <script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj('s', [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n '21-1026',\n '21-1026'\n );\n });\n</script> -->\n\n<style>\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* //////////// */\n\n /* BIOZ */\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n /* span.bioz-w-parent-hover {\n font-size: 15px;\n }\n\n td .bioz-w-tooltipx {\n padding-top: 0 !important;\n } */\n\n /* .bioz-w-parent-hover:hover {\n text-decoration: underline !important;\n } */\n\n /* /////////// */\n\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* .bioz-w-header td {\n padding: 0;\n } */\n\n .epicypher-table table {\n margin-right: auto;\n margin-left: auto;\n width: 100%;\n border-top: 1px solid lightgray;\n }\n\n .epicypher-table th {\n color: #3b3a48;\n }\n\n .epicypher-table td {\n color: #3b3a48;\n font-size: 0.9rem;\n }\n\n .epicypher-table td a {\n text-decoration: underline;\n }\n\n .epicypher-table td,\n th {\n border: 0.5px solid lightgray;\n text-align: left;\n padding: 8px;\n /* white-space: nowrap; 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Description
Digitonin is a nonionic detergent used in CUTANA™ CUT&RUN and CUT&Tag genomic mapping assays to permeabilize cells, facilitating antibody and enzyme access to chromatin. Digitonin also helps prevent clumping of Concanavalin A (ConA) magnetic beads and film formation on tubes, which minimizes sample loss. For this reason, Digitonin is recommended in assay buffers for both cell and nuclei samples. Refer to the appropriate kit manual or do-it-yourself (DIY) protocol for guidance on which assay buffers require Digitonin.
Validation Data
Figure 1: Cell permeabilization with
Digitonin
The minimum amount of CUTANA™ 5% Digitonin needed to permeabilize K562 cells was determined by
serial dilution of 5% Digitonin in Wash Buffer. Trypan Blue staining was used to evaluate
permeability with an automated cell counter (live/dead cell viability analysis). Green cells (Trypan
negative) are intact, whereas permeabilized/dead cells (Trypan positive) are red. Values (bottom
right of each panel) indicate percent of dead/permeabilized cells
Recommended Accessory Products
Item | Cat. No. |
---|---|
CUTANA™ ChIC/CUT&RUN Kit | 14-1048/14-1048-24 |
CUTANA™ CUT&RUN Library Prep Kit | 14-1001/14-1002 |
CUTANA™ CUT&Tag Kit | 14-1102/14-1103 |
CUTANA™ pAG-MNase for ChIC/CUT&RUN | 15-1016/15-1116 |
CUTANA™ pAG-Tn5 for CUT&Tag | 15-1017/15-1117 |
CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 21-1401/21-1411 |
CUTANA™ Nuclei Extraction Buffer | 21-1026 |
CUTANA™ E. coli Spike-in DNA | 18-1401 |
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes | 10-0008/10-0012 |
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009 |
Technical Information
See www.epicypher.com/protocols for detailed protocols. Visit support.epicypher.com for guidance on optimizing digitonin concentration.