CUTANA™

CUTANA™ 5% Digitonin

$145.00
SKU: 21-1004
Pack Size: 1 mL

Description

Digitonin is a nonionic detergent used in CUTANA™ CUT&RUN and CUT&Tag genomic mapping assays to permeabilize cells, facilitating antibody and enzyme access to chromatin. Digitonin also helps prevent clumping of Concanavalin A (ConA) magnetic beads and film formation on tubes, which minimizes sample loss. For this reason, Digitonin is recommended in assay buffers for both cell and nuclei samples. Refer to the appropriate kit manual or do-it-yourself (DIY) protocol for guidance on which assay buffers require Digitonin.

Validation Data

Figure 1: Cell permeabilization with Digitonin
The minimum amount of CUTANA™ 5% Digitonin needed to permeabilize K562 cells was determined by serial dilution of 5% Digitonin in Wash Buffer. Trypan Blue staining was used to evaluate permeability with an automated cell counter (live/dead cell viability analysis). Green cells (Trypan negative) are intact, whereas permeabilized/dead cells (Trypan positive) are red. Values (bottom right of each panel) indicate percent of dead/permeabilized cells

Figure 2: Quantification of cell permeabilization with Digitonin
Quantification of live/dead cells as shown in Figure 1. 0.01% was selected as the optimal concentration of Digitonin to efficiently permeabilize cells.

Recommended Accessory Products

Item Cat. No.
CUTANA ChIC/CUT&RUN Kit 14-1048/14-1048-24
CUTANA CUT&RUN Library Prep Kit 14-1001/14-1002
CUTANA CUT&Tag Kit 14-1102/14-1103
CUTANA pAG-MNase for ChIC/CUT&RUN 15-1016/15-1116
CUTANA pAG-Tn5 for CUT&Tag 15-1017/15-1117
CUTANA Concanavalin A Conjugated Paramagnetic Beads 21-1401/21-1411
CUTANA Nuclei Extraction Buffer 21-1026
CUTANA E. coli Spike-in DNA 18-1401
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes 10-0008/10-0012
CUTANA CUT&RUN 8-strip 0.2 mL Tubes 10-0009

Technical Information

Storage
Stable for 2 years at -20°C from date of receipt.
Formulation
5% (w/v) digitonin in DMSO.
Instructions for Use
CUTANA™ 5% Digitonin can be used to prepare buffers for use in CUT&RUN or CUT&Tag. If using intact cells, the concentration of digitonin should be optimized for efficient cell permeabilization. To avoid digitonin precipitation and cell lysis, use the minimal concentration of digitonin required for efficient cell permeabilization. EpiCypher has found 0.01% Digitonin to be the optimal concentration for most cell types.
See www.epicypher.com/protocols for detailed protocols. Visit support.epicypher.com for guidance on optimizing digitonin concentration.

Documents & Resources

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