CUTANA™

CUTANA™ Bead Activation Buffer

$145.00
SKU: 21-1001
Pack Size: 48 Reactions

Description

CUTANA Bead Activation Buffer is used to prepare Concanavalin A (ConA) conjugated paramagnetic beads for CUT&RUN and CUT&Tag. Activated ConA beads are used in both CUT&RUN and CUT&Tag workflows to immobilize cells or nuclei.

Validation Data

Figure 1: Cell binding to activated ConA beads
ConA beads were washed with CUTANA™ Bead Activation Buffer and loaded with K562 cells as described in the CUTANA™ CUT&RUN and CUT&Tag protocols (www.epicypher.com/protocols). Trypan Blue staining shows cells before (panels A and B) and after (C and D) bead binding. After incubation with activated beads, the supernatant contains few unbound cells (C), demonstrating efficient binding. Permeabilized cells bound to activated beads are observed in the cell-bead slurry (D).

Recommended Accessory Products

Item Cat. No.
CUTANA ChIC/CUT&RUN Kit 14-1048/14-1048-24
CUTANA CUT&RUN Library Prep Kit 14-1001/14-1002
CUTANA CUT&Tag Kit 14-1102/14-1103
CUTANA pAG-MNase for ChIC/CUT&RUN 15-1016/15-1116
CUTANA pAG-Tn5 for CUT&Tag 15-1017/15-1117
CUTANA Concanavalin A Conjugated Paramagnetic Beads 21-1401/21-1411
CUTANA Nuclei Extraction Buffer 21-1026
CUTANA E. coli Spike-in DNA 18-1401
Magnetic Separation Rack, 0.2 mL/1.5 mL Tubes 10-0008/10-0012
CUTANA CUT&RUN 8-strip 0.2 mL Tubes 10-0009

Technical Information

Storage
Stable for 12 months at 4°C from date of receipt.
Formulation
20 mM HEPES pH 7.9, 10 mM KCl, 1 mM CaCl2, 1 mM MnCl2.
Instructions for Use
CUTANA™ Bead Activation Buffer includes sufficient buffer for 48 CUT&RUN or CUT&Tag reactions using the CUTANA™ protocols (www.epicypher.com/protocols).

Documents & Resources

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