SKU: 13-0061
{"url":"https://www.epicypher.com/products/antibodies/h3-3-antibody-snap-certified-for-cut-run","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":1223,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Rabbit monoclonal histone H3.3 antibody rigorously tested for robust and reliable performance in CUT&RUN.","category":["Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUTANA™ CUT&RUN Antibodies to Histone PTMs","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1223/1243/EPI_HisVariantAb_Icon__07580.1728401254.jpg?c=2","alt":"H3.3 Antibody, SNAP-Certified™ for CUT&RUN"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=1223","shipping":{"calculated":true},"num_reviews":0,"weight":"0.00 LBS","custom_fields":[{"id":"1335","name":"Pack Size","value":"100 µg"}],"sku":"13-0061","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Monoclonal [2893-4D5]\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Wide Range (Predicted)\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Protein A affinity-purified\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>15 kDa\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n H3.3 Antibody, SNAP-Certified™ for CUT&RUN is an antibody to histone variant H3.3. H3.3 is enriched in\n promoters and enhancers of active genes as well as transcriptionally inactive heterochromatin, reflecting\n the diverse functions of this unique histone variant [1]. The antibody is highly specific for H3.3 without\n cross-reacting with canonical H3.1. Binding is not impacted by methylation or mutations at amino acids\n H3.3K27 or H3.3K36 (<strong>Figure 1</strong>).\n </p>\n <p>\n H3.3 differs from canonical H3.1 at just five amino acids (31, 87, 89, 90, 96). Most of these changes are\n located in the inaccessible histone core, making them unfit epitopes for chromatin mapping experiments. The\n SNAP-Certified™ H3.3 antibody specifically interacts with amino acid 31, where a serine is substituted for\n alanine, allowing clear distinction between H3.3 and H3.1 in chromatin mapping experiments.\n </p>\n <p>\n The H3.3 antibody meets EpiCypher’s SNAP-Certified™ criteria for specificity and efficiency in CUT&RUN. This\n requires <20% cross-reactivity to other histone variants, determined using SNAP-CUTANA™ Spike-in Controls\n (<strong>Figure 2</strong>). High target efficiency is confirmed by consistent genomic enrichment at 500k\n and 50k cells\n (<strong>Figure 3</strong>).\n </p>\n <p>\n <em>Note: This antibody is sensitive to dithiothreitol (DTT).</em>\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data - CUT&RUN</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/antibodies/13-0061-luminex-multiplexed-specificity.jpeg\"\n target=\"_blank\" class=\"image-picker__main-image-link\"><img loading=\"lazy\"\n alt=\"13-0061-luminex-multiplexed-specificity\"\n src=\"/content/images/products/antibodies/13-0061-luminex-multiplexed-specificity.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: Luminex multiplexed specificity\n profiling</strong><br />\n Histone H3.3 antibody was assessed using a Luminex® multiplexed binding assay. The panel of targets\n comprises biotinylated designer nucleosomes individually coupled to color-coded Luminex MagPlex®\n beads. Antibody binding to nucleosomes was tested in multiplex at a 1:250 dilution and detected with\n anti-IgG*Phycoerythrin (BioLegend 406421). Mean fluorescence intensity was quantified using Luminex\n FlexMAP 3D®. H3.3 antibody does not bind H3.1 and H3.3S31phos, as expected. H3.3 antibody binding is\n not affected by mutations or modifications of adjacent residues.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/antibodies/13-0061-cut-and-run-specificity-analysis.jpeg\"\n target=\"_blank\" class=\"image-picker__main-image-link\"><img loading=\"lazy\"\n alt=\"13-0061-cut-and-run-specificity-analysis\"\n src=\"/content/images/products/antibodies/13-0061-cut-and-run-specificity-analysis.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: SNAP specificity analysis in\n CUT&RUN</strong><br />\n CUT&RUN was performed as described in <b>Figure 4</b>. CUT&RUN sequencing reads were aligned to the\n unique\n DNA barcodes corresponding to each nucleosome in the variant panel (x-axis). Data are expressed as a\n percent relative to on-target recovery (H3.3 set to 100%).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/antibodies/13-0061-cut-and-run-representative-browser-tracks.jpeg\"\n target=\"_blank\" class=\"image-picker__main-image-link\">\n <img loading=\"lazy\" alt=\"13-0061-cut-and-run-representative-browser-tracks\"\n src=\"/content/images/products/antibodies/13-0061-cut-and-run-representative-browser-tracks.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: H3.3 CUT&RUN representative browser\n tracks</strong><br />\n CUT&RUN was performed as described in <b>Figure 4</b>. Gene browser shots were generated using the\n Integrative Genomics Viewer (IGV, Broad Institute). Two representative loci of the top called peaks\n are shown. Similar results in peak structure and location were observed for both 500k and 50k cell\n inputs.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/methods/cut-and-run-methods.png\" target=\"_blank\"\n class=\"image-picker__main-image-link\">\n <img loading=\"lazy\" alt=\"cut-and-run-methods\"\n src=\"/content/images/products/methods/cut-and-run-methods.png\" class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 4: CUT&RUN methods</strong><br />\n CUT&RUN was performed on 500k and 50k K562 cells with the SNAP-CUTANA™ K-MetStat Panel (EpiCypher\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\" target=\"_blank\">19-1002</a>) and Histone\n Variant Panel spiked-in prior to the addition of 0.5 µg of either IgG negative\n control (EpiCypher <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n target=\"_blank\">13-0042</a>), H3K4me3 positive control (EpiCypher <a\n href=\"/products/antibodies/h3k4me3-antibody-snap-certified-for-cut-run-and-cut-tag\"\n target=\"_blank\">13-0060</a>), or H3.3 antibodies. The\n experiment was performed using the CUTANA™ ChIC/CUT&RUN Kit v4 (EpiCypher <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n target=\"_blank\">14-1048</a>). Library\n preparation was performed with 5 ng of CUT&RUN enriched DNA (or the total amount recovered if less\n than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n target=\"_blank\">14-1001/14-1002</a>). Both kit protocols\n were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina\n NextSeq2000\n with paired-end sequencing (2x50 bp). Sample sequencing depth was 5.2 million reads (IgG 500k cell\n input), 2.5 million reads (H3K4me3 500k cell input), 7.0 million reads (H3.3 500k cell input), and\n 3.7\n million reads (H3.3 50k cell input). Data were aligned to the hg19 genome using Bowtie2. Data were\n filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\" alt=\"13-0061-luminex-multiplexed-specificity\"\n src=\"/content/images/products/antibodies/13-0061-luminex-multiplexed-specificity.jpeg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" />\n <img loading=\"lazy\" alt=\"13-0061-cut-and-run-specificity-analysis\"\n src=\"/content/images/products/antibodies/13-0061-cut-and-run-specificity-analysis.jpeg\"\n class=\"image-picker__side-image\" role=\"button\" />\n <img loading=\"lazy\" alt=\"13-0061-cut-and-run=representative-browser-tracks\"\n src=\"/content/images/products/antibodies/13-0061-cut-and-run-representative-browser-tracks.jpeg\"\n class=\"image-picker__side-image\" role=\"button\" />\n <img loading=\"lazy\" alt=\"cut-and-run-methods\"\n src=\"/content/images/products/methods/cut-and-run-methods.png\" class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A synthetic peptide corresponding to the N-terminal tail (including amino acid 31) of histone H3.3\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Protein A affinity-purified recombinant monoclonal antibody in Borate buffered saline pH\n 8.0, 0.09% sodium azide\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>CUT&RUN:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 0.5 µg per reaction\n </div>\n </div>\n <br>\n <div class=\"product-tech-info__line-item\">\n <p>\n <em>Note: Not recommended for use with buffers containing reducing agents.</em>\n </p>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Uniprot ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n H3.3 - P84243\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Cohen & Meshorer <em>Trends Cell Biol.</em> (2024). PMID:\n <a href=\"https://pubmed.ncbi.nlm.nih.gov/38614918/\" target=\"_blank\"\n title=\"H3K27me3-rich genomic regions can function as silencers to repress gene expression via chromatin interactions\">38614918</a>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/13-0061.pdf\" target=\"_blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$525.00","value":525,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H3.3 Antibody | SNAP-Certified for CUT&RUN and CUT&Tag","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":760,"sku":"13-0041","name":"H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN","url":"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/SNAP-CUTANA™ Spike-in Controls","Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUTANA™ CUT&RUN Antibodies to Histone PTMs","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n Type: Mixed Monoclonal*\n Host: Rabbit\n Applications: CUT&RUN, ICC/IF, WB ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/760/743/snap-chip-ab__70507.1557259520.1280.1280__90586.1575483123.1280.1280__61997.1592491196.png?c=2","alt":"H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/760/743/snap-chip-ab__70507.1557259520.1280.1280__90586.1575483123.1280.1280__61997.1592491196.png?c=2","alt":"H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN"}],"date_added":"17th Jun 2020","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":684,"name":"Pack Size","value":"100 µg"},{"id":685,"name":"Internal Comment","value":"extra box in top shelf of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=760","price":{"without_tax":{"currency":"USD","formatted":"$525.00","value":525},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=760"},{"id":984,"sku":"13-0058","name":"H3K36me3 Antibody, SNAP-Certified™ for CUT&RUN","url":"https://www.epicypher.com/products/antibodies/cut-and-run-antibodies/cut-and-run-antibodies-histone-ptms/h3k36me3-antibody-snap-certified-for-cut-and-run","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type: Monoclonal [2091-1E2]\n \n \n Host: Rabbit\n \n \n Applications: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/984/1114/snap-chip-ab__70507.1557259520.1280.1280__90586.1575483123.1280.1280__61997.1592491196__58196.1676557361.png?c=2","alt":"H3K36me3 Antibody, SNAP-Certified™ for CUT&RUN"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/984/1114/snap-chip-ab__70507.1557259520.1280.1280__90586.1575483123.1280.1280__61997.1592491196__58196.1676557361.png?c=2","alt":"H3K36me3 Antibody, SNAP-Certified™ for CUT&RUN"}],"date_added":"16th Feb 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Pack Size: 100 µg
