SKU: 16-0016
{"url":"https://www.epicypher.com/products/nucleosomes/recombinant-nucleosomes-rnucs/mononucleosomes-h3-1-n32-recombinant-human-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":514,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated for Enzyme Screening","category":["Nucleosomes/Recombinant Nucleosomes (rNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/514/360/Histone_variant_nucleosome__65083.1515789648.1280.1280__51889.1520432823.gif?c=2","alt":"Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=514","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"606","name":"Pack Size","value":"50 µg"}],"sku":"16-0016","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong><em>E. coli</em> & synthetic DNA\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Tag: </strong>Biotinylated\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Molecular Weight: </strong>193,951.1 Da\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n Recombinant mononucleosomes (H3.1ΔN32) consist of 147 base pairs of\n DNA wrapped around an octamer core of histone proteins (two each of\n H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating\n unit of chromatin. The 147 bp 601 sequence, identified by Lowary and\n Widom [1], has high affinity for histone octamers and is useful for\n nucleosome assembly. The amino acid sequence of H3.1 begins with\n glycine 33 (amino acids 1-32 are deleted) and the DNA in this\n nucleosome contains a 5’ biotin-TEG group.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0016-western-blot-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0016-western-blot-data\"\n src=\"/content/images/products/nucleosomes/16-0016-western-blot-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Western blot data </strong><br />\n Western analysis of H3.1ΔN32 nucleosome.\n <strong>Top Panel:</strong> WT H3.1 (Lane 1) and\n H3.1ΔN32-containing (Lane 2) nucleosomes were probed with an\n anti-H3 COOH-terminal antibody and analyzed via ECL readout.\n <strong>Bottom Panel:</strong> Detail from Coomassie stained\n gel showing histones from WT H3.1 (Lane 1) and H3.1ΔN32\n nucleosomes (Lane 2). H3.1ΔN32 and H4 co-migrate.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0016-protein-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0016-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0016-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein gel data </strong><br />\n Coomassie stained SDS-PAGE gel of proteins in H3.1ΔN32\n nucleosome (1 μg) demonstrates the purity of histones in the\n preparation. Sizes of molecular weight markers and positions\n of the core histones (H2A, H2B, H3.1ΔN32 and H4) are\n indicated. H3.1ΔN32 and H4 co-migrate.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0016-dna-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0016-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0016-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 3: DNA gel data </strong><br />\n H3.1ΔN32 nucleosome resolved via native PAGE and stained\n with ethidium bromide to visualize DNA.\n <strong>Lane 1</strong>: Free DNA (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\"\n >18-0005</a\n >; 100 ng). <strong>Lane 2:</strong> Intact H3.1ΔN32\n nucleosomes (400 ng).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"16-0016-western-blot-data\"\n src=\"/content/images/products/nucleosomes/16-0016-western-blot-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"16-0016-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0016-protein-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"16-0016-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0016-dna-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For best\n results, aliquot and avoid freeze/thaws.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, 20%\n glycerol (27.4 µg protein, 50 µg DNA + protein)\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n H3.1ΔN32 mononucleosome is highly purified and suitable for a\n variety of applications, including use as a substrate in enzyme\n assays, high-throughput screening and inhibitor testing, chromatin\n binding studies, protein-protein interaction assays, structural\n studies, and in effector protein binding experiments. The N-terminal\n deletion enables study of its role in chromatin biology.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n a\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin-bottom: 0\">\n H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)\n </p>\n <p style=\"margin-bottom: 0\">\n H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)\n </p>\n <p style=\"margin-bottom: 0\">\n H3.1 - P68431 (alt. names: H3, H3/a, H3/b, H3/c, H3/d)\n </p>\n <p>H4 - P62805</p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n target=\"new\"\n >\n 9514715</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0016.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated for Enzyme Screening","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"Highly Purified Recombinant Nucleosomes for Enzyme Assays, Screening and Kinetics, recombinant nucleosome, recombinant chromatin, recombinant mononucleosomes, highly purified recombinant nucleosomes, human, biotinylated, 16-0016, nucleosome, n-terminal deletion, h3.1, Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated for Enzyme Screening","show_quantity_input":1,"title":"Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/514/360/Histone_variant_nucleosome__65083.1515789648.1280.1280__51889.1520432823.gif?c=2","alt":"Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated"}]}
Pack Size: 50 µg
