SKU: 16-0333
{"url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h4k20me3-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":369,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"H4K20Me3 Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/369/313/Recombinant_nucleosome__94523.1582226101.png?c=2","alt":"Nucleosome, Recombinant Human, H4K20me3 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=369","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"936","name":"Pack Size","value":"50 μg"},{"id":"937","name":"Internal Comment","value":"new lot in bottom right of psylocke."}],"sku":"16-0333","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n<b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H4K20me3</b></span></span> dNuc Description:</b> Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli </em>(two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H4 (created by a proprietary synthetic method) contains\ntrimethyl-lysine at position 20. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H4K20me3</b></span></span> dNuc Formulation:</b> Recombinant Human, H4K20me3 (27 μg\nprotein weight, 50.0 μg DNA+protein) in 10 mM\nTris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20%\nglycerol. MW = 201,036 Da.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H4K20me3 dNuc Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H4K20me3 dNuc Application Notes:</b> Nucleosome, Recombinant Human, H4K20me3 are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. <strong>Nucleosome, Recombinant Human, H4K20me3 from EpiCypher does not contain free DNA which could alter assayed activities.</strong><br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0333.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0333 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0333-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0333-WB-data.jpg\" alt=\"16-0333 Western Blot\" height=\"125\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong> Western Analysis of Nucleosome, Recombinant Human, H4K20me3. <strong>Top Panel:</strong> Unmodified H4 (Lane 1) and H4K20me3 containing nucleosomes (Lane 2) were probed with an anti-H4K20me3 antibody and analyzed via ECL readout. Only the H4K20me3 sample produced a detectable signal. <strong>Bottom Panel: </strong>Detail from Coomassie stained protein gel showing unmodified nucleosomes (Lane 1) and H4K20me3 nucleosomes.<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0333-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0333-MS-data.jpg\" alt=\"16-0333 MS Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> Synthetic H4K20me3 protein analyzed by ESI-TOF mass spectrometry. Expected mass = 11278.1 Da. Determined mass = 11276.9 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0333-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0333-protein-gel.jpg\" alt=\"16-0333 Protein Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H4K20me3 (2 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H4K20me3 and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0333-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0333-DNA-gel.jpg\" alt=\"16-0333 Protein Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H4K20me3 run on a non-denaturing acrylamide gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1: </strong>Free DNA extracted from nucleosomes (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> </td></tr></tbody></table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H4K20Me3 Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"designer nucleosome, dNuc, H4K20Me3, h4 K20 trimethyl, h4 K20 trimethyl, nucleosome, mononucleosome, 16-0333, histone h4, dnuc, recombinant nucleosome","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H4K20me3 dNuc, Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/369/313/Recombinant_nucleosome__94523.1582226101.png?c=2","alt":"Nucleosome, Recombinant Human, H4K20me3 dNuc, Biotinylated"}]}
Pack Size: 50 μg
