SKU: 16-1313
{"url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h4-tetraacetyl-k5-8-12-16ac-dnuc","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":535,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"H4 Tetraacetyl Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","category":["Discontinued Products"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/535/383/Recombinant_nucleosome__78815.1525791275.png?c=2","alt":"Nucleosome, Recombinant Human, H4 Tetraacetyl (K5,8,12,16Ac) dNuc"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=535","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"264","name":"Pack Size","value":"50 μg"}],"sku":"16-1313","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\">\n \n <tbody>\n \n <tr valign=\"top\">\n \n <td>\n \n \n \n <table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\">\n \n <tbody>\n \n \n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n \n <b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H4 Tetraacetyl</b></span></span> dNuc Description:</b> Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H4 (created by a proprietary synthetic method) contains N-terminal alpha-acetylation and acetyl-lysine at positions 5, 8, 12 and 16. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H4 Tetraacetyl</b></span></span> dNuc Formulation:</b> Nucleosome, Recombinant Human, H4 Tetraacetyl <span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \">(<span class=\"Apple-style-span\" style=\"font-size: 12pt; \">50 µg total mass, DNA+protein</span>, 27.3 µg protein weight)</span> in</span> 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 201,031 Da.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H4 Tetraacetyl dNuc Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H4 Tetraacetyl dNuc Application Notes:</b> Nucleosome, Recombinant Human, H4 Tetraacetyl are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein (especially bromodomain) binding experiments. <strong>Nucleosome, Recombinant Human, H4 Tetraacetyl from EpiCypher does not contain free DNA which could alter assayed activities.</strong><br />\n \n <br />\n \n </span></span>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\">\n \n <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br />\n <br />\n \n </span></span><br />\n \n </p>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0313.pdf\" target=\"_new\">\n \n <img src=\"/content/documents/tds/icon.png\" alt=\"15-1010 Datasheet\" width=\"30\" height=\"40\" /></a></span></span>\n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n </td>\n \n <td>\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0313-MS.jpg\" target=\"_new\">\n \n <img src=\"/content/images/products/nucleosomes/16-0313-MS.jpg\" alt=\"16-0313 dNuc Mass Spec Gel Data\" height=\"150\" width=\"150\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n \n \n \n \n \n <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> Synthetic H4 Tetraacetyl protein analyzed by ESI-TOF mass spectrometry. Expected mass = 11,446.2 Da. Determined mass = 11,446.0 Da.<br />\n \n <strong>(Click image to enlarge) </strong></span></span></p>\n \n \n \n \n \n \n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n<table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0313-protein-gel.jpg\" target=\"_new\">\n \n <img src=\"/content/images/products/nucleosomes/16-0313-protein-gel.jpg\" alt=\"16-0313 dNuc Protein Gel Data\" height=\"325\" width=\"150\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n \n \n \n \n \n <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H4 Tetraacetyl (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H4 Tetraacetyl and H4) are indicated.<br />\n \n <strong>(Click image to enlarge) </strong></span></span></p>\n \n \n \n \n \n \n \n </td>\n \n </tr>\n \n </tbody>\n \n </table>\n \n \n \n <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\">\n \n <tbody>\n \n <tr>\n \n <td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0313-DNA-gel.jpg\" target=\"_new\">\n \n <img src=\"/content/images/products/nucleosomes/16-0313-DNA-gel.jpg\" alt=\"16-0313 dNuc DNA Gel Data\" height=\"125\" /></a>\n \n </td>\n \n </tr>\n \n <tr>\n \n <td align=\"left\" valign=\"top\">\n \n \n \n \n \n \n <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H4 Tetraacetyl run on an agarose gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1: </strong>Free DNA extracted from nucleosomes (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).<br />\n \n <strong>(Click image to enlarge) </strong></span></span></p>\n \n \n \n \n \n \n \n </td>\n \n </tr>\n \n </tbody>\n \n </table> \n \n \n \n \n \n \n \n \n \n \n \n \n \n </td>\n \n </tr>\n \n </tbody>\n\n</table>\n\n\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H4 Tetraacetyl Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"designer nucleosome, dNuc, H4 K5,8,12,16Ac, h4 K5,8,12,16 acetyl, nucleosome, mononucleosome, h4 tetracetyl dnuc, dnucs, tetraacetyl, 16-0313","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H4 Tetraacetyl (K5,8,12,16Ac) dNuc","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/535/383/Recombinant_nucleosome__78815.1525791275.png?c=2","alt":"Nucleosome, Recombinant Human, H4 Tetraacetyl (K5,8,12,16Ac) dNuc"}]}
Pack Size: 50 μg
