SKU: 16-0341
{"url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3r2me2a-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":381,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"H3R2me2a Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/381/321/Recombinant_nucleosome__29081.1582226102.png?c=2","alt":"Nucleosome, Recombinant Human, H3R2me2a dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=381","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1066","name":"Pack Size","value":"50 μg"},{"id":"1067","name":"Internal Comment","value":"Has Bulk in LG"}],"sku":"16-0341","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n<b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3R2me2a</b></span></span> dNuc Description:</b> Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains dimethyl-arginine at position 2. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group. * H3R2me2a has a Cys to Ala substitution at position 110.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3R2me2a</b></span></span> dNuc Formulation:</b> Nucleosome, Recombinant Human, H3R2me2a (27.3 µg protein weight, 50 µg total weight) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 200,479 Da.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3R2me2a dNuc Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3R2me2a dNuc Application Notes:</b> Nucleosome, Recombinant Human, H3R2me2a dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. <strong>Nucleosome, Recombinant Human, H3R2me2a from EpiCypher does not contain free DNA which could alter assayed activities.</strong><br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0341.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0341 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0341-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0341-WB-data.jpg\" alt=\"16-0341 Western Blot\" width=\"125\" height=\"300\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong> Western Analysis of Nucleosome, Recombinant Human, H3R2me2a. <strong>Top Panel:</strong> Unmodified H3 (Lane 1) and H3R2me2a containing nucleosomes (Lane 2) were probed with an anti-H3R2me2a antibody and analyzed via ECL readout. Only the H3R2me2a sample produced a detectable signal. <strong>Bottom Panel:</strong> Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H3R2me2a nucleosomes (Lane 2).<br /> <strong>(Click image to enlarge) \n</strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0341-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0341-MS-data.jpg\" alt=\"16-0341 MS Data\" width =\"125\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> Synthetic H3R2me2a protein analyzed by high resolution mass spectrometry. Expected mass = 15252.8 Da. Determined mass = 15,252.5 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0341-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0341-protein-gel.jpg\" alt=\"16-0341 Protein Gel Data\" width=\"125\" height=\"300\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H3R2me2a (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3R2me2a and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0341-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0341-DNA-gel.jpg\" alt=\"16-0341 Protein Gel Data\" width=\"125\" height=\"300\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H3R2me2a run on a native PAGE gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng). <br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> </td></tr></tbody></table>\n\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H3R2me2a Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":380,"sku":"16-0340","name":"Nucleosome, Recombinant Human, H3R2me1 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3r2me1-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/380/320/Recombinant_nucleosome__99765.1582226102.png?c=2","alt":"Nucleosome, Recombinant Human, H3R2me1 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/380/320/Recombinant_nucleosome__99765.1582226102.png?c=2","alt":"Nucleosome, Recombinant Human, H3R2me1 dNuc, Biotinylated"}],"date_added":"1st Aug 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":787,"name":"Pack Size","value":"50 μg"},{"id":788,"name":"Internal Comment","value":"Has Bulk - Not Included in Amount"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=380","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=380"}],"shipping_messages":[],"rating":0,"meta_keywords":"designer nucleosome, dNuc, H3R2Me2a, , H3R2Me2a, h3 r2 dimethyl asymmetric, nucleosome, mononucleosome, chromatin, 16-0341, recombinant nucleosome, h3 r2me2a","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H3R2me2a dNuc, Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/381/321/Recombinant_nucleosome__29081.1582226102.png?c=2","alt":"Nucleosome, Recombinant Human, H3R2me2a dNuc, Biotinylated"}]}
Pack Size: 50 μg
