SKU: 16-1314
{"url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k9ac-dnuc","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":496,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"H3 K9ac Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/496/338/Recombinant_nucleosome__97390.1516390304.png?c=2","alt":"Nucleosome, Recombinant Human, H3K9ac dNuc"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=496","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"239","name":"Pack Size","value":"50 μg"}],"sku":"16-1314","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br />\n<b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K9ac dNuc</b></span></span> Description:</b> Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains acetyl-lysine at position 9. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly. *H3K9ac has a Cys to Ala substitution at position 110.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K9ac dNuc</b></span></span>Formulation:</b> Nucleosome, Recombinant Human, H3K9ac (27.3 µg protein weight, 50 µg total weight) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 199,822 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K9ac dNuc Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K9ac dNuc Application Notes:</b> Nucleosome, Recombinant Human, H3K9ac are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. <strong>Nucleosome, Recombinant Human, H3K9ac dNucs from EpiCypher does not contain free DNA which could alter assayed activities.</strong><br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-1314.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-1314 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-1314-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-1314-WB-data.jpg\" alt=\"16-1314 Western Blot\" height=\"125\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong> Western Analysis of Nucleosome, Recombinant Human, H3K9ac. <strong>Top Panel:</strong> Unmodified H3 (Lane 1) and H3K9ac containing nucleosomes (Lane 2) were probed with an anti-H3K9ac antibody and analyzed via ECL readout. Only the H3K9ac sample produced a detectable signal. <strong>Bottom Panel:</strong> Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H3K9ac nucleosomes (Lane 2).<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-1314-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-1314-MS-data.jpg\" alt=\"16-1314 MS Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> Synthetic H3K9ac protein analyzed by ESI-TOF mass spectrometry. Expected mass = 15266.8 Da. Determined mass = 15265.5 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-1314-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-1314-protein-gel.jpg\" alt=\"16-1314 Protein Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained SDS-PAGE gel of proteins in Nucleosome, Recombinant Human, H3K9ac (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K9ac and H4 are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-1314-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-1314-DNA-gel.jpg\" alt=\"16-1314 Protein Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H3K9ac examined by native PAGE and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (100 ng). <strong>Lane 2:</strong> Intact Nucleosome, Recombinant Human, H3K9ac (400 ng).<br /> <strong>(Click image to enlarge) 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Pack Size: 50 μg
