SKU: 16-0346
{"url":"https://www.epicypher.com/products/nucleosomes/mutant-nucleosomes/mononucleosomes-h3-3g34r-recombinant-human-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":717,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Mononucleosomes (H3.3G34R), Recombinant Human, Biotinylated","category":["Nucleosomes/Mutant Nucleosomes","Nucleosomes/Mutant Nucleosomes/Oncogenic Nucleosomes (oncoNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/717/672/oncoNuc__56863.1519695540.1280.1280__84598.1578598419.gif?c=2","alt":"Mononucleosomes (H3.3G34R), Recombinant Human, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=717","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"486","name":"Pack Size","value":"50 µg"}],"sku":"16-0346","description":"\n\n\n<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody>\n\n<tr valign=\"top\"> \n\n<td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> \n\n\n\n<tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3G34R), Recombinant Human </strong><strong style=\"font-family: Arial; font-size: 12pt;\">Description: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing an arginine at position 34, expressed in <i>E. coli</i>. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA containing a 5' biotin-TEG group wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes).</span><br /> \n <br />\n \n\n </p></td> \n</tr> <tr> \n \n \n \n \n \n \n \n \n \n \n <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3G34R), Recombinant Human Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Mononucleosomes (H3.3G34R), Recombinant Human (27.3 µg protein weight, 50 µg DNA+protein) in 10mM Tris HCl, pH 7.5, 25mM NaCl, 1 mM EDTA, 2mM DTT, 20% glycerol. MW = 199,905.2.<br /> <br /> </span></span></td> \n </tr> \n \n \n \n \n \n \n \n \n \n <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3G34R), Recombinant Human </strong><strong>Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3G34R), Recombinant Human </strong><strong>Application Notes:</strong> Mononucleosomes (H3.3G34R), Recombinant Human are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. <strong>EpiCypher <strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H3.3G34R), Recombinant Human </strong>do not contain free DNA which could alter assayed activities.</strong><br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <span style=\"font-size: 10pt;\">Lowary PT and J Widom (1998). <em>J Mol Bio</em>l 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a></span><br /> <span style=\"font-size: 10pt;\">Luger K et al (1999). <em>Methods Mol Biol</em> 119: 1-16. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/10804500\" target=\"_new\">Link</a></span><br /> <br /> </span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-0346.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-0346 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> \n \n \n \n <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> \n \n \n <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0346_WB_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0346_WB_tds.jpg\" alt=\"16-0346 Western Blot\" width=\"150\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-size:10pt;\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong style=\"font-family: Arial; font-size: 10pt;\">Western Blot Data:</strong> Western analysis of Mononucleosomes (H3.3G34R), Recombinant Human. <strong>Top Panel:</strong> Unmodified H3.3(Lane 1) and H3.3G34R containing nucleosomes (Lane 2) were probed with an anti-H3 G34R antibody. Only the H3.3G34R sample produced a detectable signal. <strong>Bottom Panel:</strong> Detail from Coomassie stained gel of Western blot. </span></span></td> \n </tr> \n \n \n \n <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0346_dna_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0346_dna_gel_tds.jpg\" alt=\"16-0346 DNA Gel\" width=\"281\" height=\"250\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><strong style=\"font-family: Arial; font-size: 10pt;\">DNA Gel Data:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Mononucleosomes (H3.3G34R), Recombinant Human resolved via native PAGE and stained with ethidium bromide to visualize DNA. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Lane 1:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Free DNA (100 ng)<strong style=\"font-family: Arial; font-size: 10pt;\"> Lane 2:</strong> Intact nucleosomes (400 ng). Nucleosomal DNA migrates much higher than free DNA, thus the DNA resolves at a higher molecular weight when nucleosome-bound. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Note absence of free DNA in Lane 1, indicating all DNA complexed with histones.</strong></td> \n </tr> \n \n \n \n \n <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0346_protein_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0346_protein_gel_tds.jpg\" alt=\"16-0346 Protein Gel\" width=\"200\"/></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Mononucleosomes (H3.3G34R), Recombinant Human (1 µg) to demonstrate the purity and integrity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3.3G34R and H4) are indicated. </span></span></td> \n </tr> \n \n \n \n \n \n \n </tbody> </table> </td> </tr> </tbody> </table> </div>\n\n \n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Mononucleosomes (H3.3G34R), Recombinant Human, Biotinylated","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":84,"sku":"16-0006","name":"Mononucleosomes, Recombinant Human Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)"],"summary":"\n \n \n \n Description\n \n \n Mononucleosomes 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Pack Size: 50 µg
