SKU: 16-1323
{"url":"https://www.epicypher.com/products/nucleosomes/mutant-nucleosomes/mononucleosomes-h3-3-k27m-recombinant-human-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":473,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Mononucleosomes (H3.3 K27M) Recombinant Human Biotinylated for Inhibitor Screening and Kinetics","category":["Nucleosomes/Mutant Nucleosomes/Oncogenic Nucleosomes (oncoNucs)","Nucleosomes/Mutant Nucleosomes"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/473/339/onco_Nucleosome__80920.1516390305.gif?c=2","alt":"Mononucleosomes (H3.3 K27M), Recombinant Human Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=473","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"241","name":"Pack Size","value":"50 μg"}],"sku":"16-1323","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody>\n\n<tr valign=\"top\"> \n\n<td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> \n\n\n\n<tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a methionine at position 27, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of M for K at position 27 in human H3.1 and H3.3 is a dominant negative inhibitor of the EZH2 methytransferase complex PRC2 and associated with pediatric brain cancer (DIPG). The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group. </span><br /> \n <br />\n \n</td> \n</tr> <tr> \n \n \n \n \n \n \n \n \n \n \n <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 242,900 Da.<br /> <br /> </span></span></td> \n </tr> \n \n \n \n \n \n \n \n \n \n <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated Application Notes:</strong> This product is a substrate for chromatin remodeling assays using restriction enzymes to determine accessibility of GATC subsequent to chromatin remodeling.\n <br />\n <strong><strong style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated</strong>do not contain free DNA which could alter assayed activities.</strong><br /><br /></span></span></td> </tr> \n \n \n \n \n \n <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-family: Arial; font-size: 12pt;\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC References</strong><strong style=\"font-size: 12pt;\">:</strong>\n <br /> <br /> </span></td> </tr> \n \n <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-1323.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-1323 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> \n \n \n \n \n </tbody> </table> </td> \n \n \n \n <td width=\"350px\"> <table style=\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> \n \n \n \n \n <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-1323_western_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-1323_western_tds.jpg\" alt=\"16-1323 Western Blot\" width=\"150\" /></a></td> </tr> \n \n <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong style=\"font-family: Arial; font-size: 10pt;\">Western Blot Data:</strong><span style=\"font-size: 10pt;\"> Coomassie stained PAGE gel of proteins in Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated (2 µg) demonstrates the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.</span></span></td> \n </tr> \n \n \n \n \n <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-1323_DNA_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-1323_DNA_gel_tds.jpg\" alt=\"16-1323 DNA Gel\" width=\"150\"/></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-size:10pt;\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>DNA Gel Data:</strong> Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated run using native PAGE and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA extracted from nucleosomes (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng). <strong style=\"font-family: Arial; font-size: 10pt;\"> Note absence of free DNA in "Nuc", indicating all DNA complexed with histones.</strong></span></span></td> \n </tr> \n \n\n \n \n \n \n <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-1323_protein_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-1323_protein_gel_tds.jpg\" alt=\"16-1323 Protein Gel\" width=\"150\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-size:10pt;\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong style=\"font-family: Arial; font-size: 10pt;\">Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Mononucleosomes (H3.3K27M), Recombinant Human (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3.3K27M and H4) are indicated.</span></span></td> \n </tr> \n \n \n \n \n \n \n </tbody> </table> </td> </tr> </tbody> </table> </div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Mononucleosomes (H3.3 K27M) Recombinant Human Biotinylated for Inhibitor Screening and Kinetics","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":254,"sku":"16-0012","name":"Mononucleosomes (H3.3), Human Recombinant","url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-h3-3-human-recombinant","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)","Nucleosomes/Variant Nucleosomes (vNucs)"],"summary":" Mononucleosomes (H3.3), Human Recombinant Description: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/254/330/Histone_variant_nucleosome__69550.1516390310.gif?c=2","alt":"Mononucleosomes (H3.3), Human Recombinant"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/254/330/Histone_variant_nucleosome__69550.1516390310.gif?c=2","alt":"Mononucleosomes (H3.3), Human Recombinant"}],"date_added":"3rd Dec 2015","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":128,"name":"Pack Size","value":"100 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=254","price":{"without_tax":{"currency":"USD","formatted":"$425.00","value":425},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=254"}],"shipping_messages":[],"rating":0,"meta_keywords":"Mononucleosomes (H3.3 K27M), Recombinant Human, vNuc, oncohistone, DIPG, EZH2, K27me, K27me3, 16-0323, Mononucleosomes (H3.3 K27M), Recombinant Human, vNuc, oncohistone, DIPG, EZH2, K27me, K27me3, 16-0323, h3k27m, h3.3k27m, k27m, onconuc","show_quantity_input":1,"title":"Mononucleosomes (H3.3 K27M), Recombinant Human Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/473/339/onco_Nucleosome__80920.1516390305.gif?c=2","alt":"Mononucleosomes (H3.3 K27M), Recombinant Human Biotinylated"}]}
Pack Size: 50 μg
