SKU: 16-1013
{"url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-h2ax-human-recombinant","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":532,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Human Recombinant H2AX Nucleosomes for Enzyme Screening","category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)","Nucleosomes/Variant Nucleosomes (vNucs)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/532/380/Histone_variant_nucleosome__19142.1525791282.gif?c=2","alt":"Mononucleosomes (H2AX), Human Recombinant"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=532","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"263","name":"Pack Size","value":"50 μg"}],"sku":"16-1013","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AX), Human Recombinant </strong><strong style=\"font-family: Arial; font-size: 12pt;\">Description: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli</em> (two each of histones H2AX, H2B, H3 and H4. Accession numbers: H2AX-P16104; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. H2AX is a variant of histone H2A that is phosphorylated on serine 139 by the ATM and ATR kinases in response to DNA double strand breakage. Phosphorylation of H2AX serves to recruit a number of DNA damage repair proteins, such as BRCA1 and NBS1.</span><br /> <br /> </p></td> </tr> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AX), Human Recombinant Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Purified recombinant mononucleosomes (50 µg total mass, 27.4 µg in 50.0 µl) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT and 20% glycerol. Concentration of nucleosomes is 4.94 µM. Nucleosome molecular weight = 202,308.2 Da.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AX), Human Recombinant </strong><strong>Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AX), Human Recombinant </strong><strong>Application Notes:</strong> Mononucleosomes (H2AX), Human Recombinant are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. <strong>EpiCypher <strong style=\"font-family: Arial; font-size: 16px;\">Mononucleosomes (H2AX), Human Recombinant </strong>do not contain free DNA which could alter assayed activities.</strong><br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <span style=\"font-size: 10pt;\">Lowary PT and J Widom (1998). <em>J Mol Bio</em>l 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a></span><br /> <span style=\"font-size: 10pt;\">Luger K et al (1999). <em>Methods Mol Biol</em> 119: 1-16. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/10804500\" target=\"_new\">Link</a></span><br /> <br /> </span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-1013.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-1013 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0013_protein_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0013_protein_gel_tds.jpg\" alt=\"16-0013 Protein Gel\" width=\"200\"/></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Nucleosome (H2AX), Human Recombinant (1 µg) demonstrates the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2AX, H2B, H3 and H4) are indicated. <strong>N.B.</strong> Histone H2AX co-migrates with histone H3. </span></span></td> </tr> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0013_dna_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0013_dna_gel_tds.jpg\" alt=\"16-0013 DNA Gel\" width=\"250\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><strong style=\"font-family: Arial; font-size: 10pt;\">DNA Gel Data:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Nucleosome (H2AX), Human Recombinant run on an agarose gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> DNA extracted from nucleosomes (100 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng). </span><strong style=\"font-family: Arial; font-size: 10pt;\">Note absence of free DNA in Lane 1, indicating all DNA complexed with histones.</strong></td> </tr> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-0013_WB_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-0013_WB_tds.jpg\" alt=\"16-0013 Western Blot\" width=\"175\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-size:10pt;\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong style=\"font-family: Arial; font-size: 10pt;\">Western Blot Data:</strong> Western Analysis of Nucleosome (H2AX), Human Recombinant. <strong>Top Panel:</strong> H2A-containing (Lane 1) and H2AX-containing nucleosomes (Lane 2) were probed with an anti-H2AX antibody and analyzed via ECL readout. Only the H2AX samples produced a detectable signal. <strong>Bottom Panel:</strong> Detail from Coomassie stained gel showing histones from H2A (Lane 1) and H2AX nucleosomes (Lane 2). </span></span></td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Human Recombinant H2AX Nucleosomes","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":349,"sku":"16-0013","name":"Mononucleosomes (H2AX), Human Recombinant Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/mononucleosomes-h2ax-human-recombinant-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Recombinant Nucleosomes (rNucs)","Nucleosomes/Variant Nucleosomes (vNucs)"],"summary":" 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Pack Size: 50 μg
