SKU: 16-0355
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3r2me2s-dnuc","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":595,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Nucleosome, Recombinant Human, H3R2me2s dNuc, biotinylated","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/595/544/Recombinant_nucleosome__86683.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3R2me2s dNuc"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=595","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"393","name":"Pack Size","value":"50 μg"}],"sku":"16-0355","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3R2me2s dNuc, biotinylated Description:</b> Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A,\nH2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pair of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains symmetrically dimethyl-arginine at position 2. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base\npair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains 5’ biotin-TEG group.\n*H3R2me2s has a Cys to Ala substitution at position 110.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3R2me2s</b></span></span> dNuc BiotinylatedFormulation:</b> Nucleosome, Recombinant Human, H3R2me2s (27.3 μg protein weight, 50 μg total weight) in 65.6 μl of 10 mM\nTris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 200,479 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3R2me2s dNuc Biotinylated Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For\nbest results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3R2me2s dNuc Biotinylated Application Notes:</b> Nucleosome, Recombinant Human, H3R2me2s dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3R2me2s dNucs from EpiCypher does not contain free DNA which could alter assayed activities.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0355.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0355 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0355-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0355-WB-data.jpg\" alt=\"16-0355 Western Blot\" width=\"125\" height=\"325\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong>Western Analysis of Nucleosome,<br />\n Recombinant Human, H3R2me2s. Top Panel:</strong> Unmodified H3 (Lane<br />\n 1) and H3R2me2s containing nucleosomes (Lane 2) were probed with an anti-H3R2me2s antibody and analyzed via ECL readout. Only the H3R2me2s sample produced a detectable signal. Bottom Panel: Detail Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H3R2me2s nucleosomes (Lane 2).<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0355-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0355-MS-data.jpg\" alt=\"16-0355 MS Data\" width=\"125\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong>H3R2me2s protein analyzed by high resolution mass spectrometry. Expected mass = 15252.8 Da. Determined<br />\n mass = 15,252.5 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0355-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0355-protein-gel.jpg\" alt=\"16-0355 Protein Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in\nNucleosome, Recombinant Human, H3R2me2s (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A,H2B, H3R2me2s and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0355-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0355-DNA-gel.jpg\" alt=\"16-0355 DNA Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H3R2me2s run on a native PAGE gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> </td></tr></tbody></table>\n\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Nucleosome, Recombinant Human, H3R2me2s dNuc","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":520,"sku":"16-1320","name":"Nucleosome, Recombinant Human, H3K36me3 dNuc","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k36me3-dnuc","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/520/368/Recombinant_nucleosome__32026.1523638379.png?c=2","alt":"Nucleosome, Recombinant Human, H3K36me3 dNuc"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/520/368/Recombinant_nucleosome__32026.1523638379.png?c=2","alt":"Nucleosome, Recombinant Human, H3K36me3 dNuc"}],"date_added":"9th Mar 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":251,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=520","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=520"},{"id":513,"sku":"16-1365","name":"Nucleosome, Recombinant Human, H3K27ac dNuc","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k27ac-dnuc","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/513/357/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520394110.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27ac dNuc"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/513/357/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520394110.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27ac dNuc"}],"date_added":"6th Mar 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":597,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=513","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=513"},{"id":522,"sku":"16-1345","name":"Nucleosome, Recombinant Human, H3S10phos dNuc","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3s10phos-dnuc","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/522/370/Recombinant_nucleosome__21054.1523638366.png?c=2","alt":"Nucleosome, Recombinant Human, H3S10phos dNuc"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/522/370/Recombinant_nucleosome__21054.1523638366.png?c=2","alt":"Nucleosome, Recombinant Human, H3S10phos dNuc"}],"date_added":"10th Mar 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":253,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=522","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=522"},{"id":521,"sku":"16-1333","name":"Nucleosome, Recombinant Human, H4K20me3 dNuc","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h4k20me3-dnuc","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/521/369/Recombinant_nucleosome__65791.1523638366.png?c=2","alt":"Nucleosome, Recombinant Human, H4K20me3 dNuc"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/521/369/Recombinant_nucleosome__65791.1523638366.png?c=2","alt":"Nucleosome, Recombinant Human, H4K20me3 dNuc"}],"date_added":"10th Mar 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":252,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=521","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=521"},{"id":497,"sku":null,"name":"Nucleosome, Recombinant Human, H3K4me2 dNuc","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k4me2-dnuc","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/497/337/Recombinant_nucleosome__81635.1516390310.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me2 dNuc"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/497/337/Recombinant_nucleosome__81635.1516390310.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me2 dNuc"}],"date_added":"20th Oct 2017","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":null,"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$295.00","value":295},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=497"}],"shipping_messages":[],"rating":0,"meta_keywords":"Nucleosome, Recombinant Human, H3R2me2s dNuc, biotinylated","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H3R2me2s dNuc","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/595/544/Recombinant_nucleosome__86683.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3R2me2s dNuc"}]}
Pack Size: 50 μg
