SKU: 16-0351
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k9cr-dnuc","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":599,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Nucleosome, Recombinant Human, H3K9cr dNuc","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/599/548/Recombinant_nucleosome__75275.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3K9cr dNuc"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=599","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"809","name":"Pack Size","value":"50 μg"},{"id":"810","name":"Internal Comment","value":"extra in bottom right of Psylocke"}],"sku":"16-0351","description":"<table width=\"100%\" cellspacing=\"2\" cellpadding=\"2\">\n <tbody>\n <tr valign=\"top\">\n <td>\n <table width=\"90%\" cellspacing=\"1\" cellpadding=\"1\">\n <tbody>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 12pt\" class=\"Apple-style-span\">\n <span class=\"Apple-style-span\" style=\"font-family: Arial\">\n <span class=\"Apple-style-span\" style=\"font-size: 12pt\">\n <b>\n Nucleosome, Recombinant Human, H3K9cr dNuc\n Description:</b\n >\n Mononucleosomes assembled from recombinant human\n histones expressed in E. coli (two each of histones H2A,\n H2B, H3 and H4; accession numbers: H2A-P04908;\n H2BO60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base\n pairs of 601 positioning sequence DNA. Histone H3\n (created by a proprietary semi-synthetic method)\n contains crotonyl-lysine at position 9. The nucleosome\n is the basic subunit of chromatin. The 601 sequence,\n identified by Lowary and Widom, is a 147-base pair\n sequence that has high affinity for histone octamers and\n is useful for nucleosome assembly and contains a 5’\n biotin-TEG group. * H3K9cr has a Cys to Ala substitution\n at position 110.<br />\n <br />\n </span>\n </span>\n </span>\n </span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 12pt\" class=\"Apple-style-span\">\n <b>\n <span class=\"Apple-style-span\" style=\"font-family: Arial\">\n <span class=\"Apple-style-span\" style=\"font-size: 12pt\">\n <b>Nucleosome, Recombinant Human, H3K9cr</b>\n </span>\n </span>\n dNuc Biotinylated Formulation:</b\n >\n Nucleosome, Recombinant Human, H3K9cr (27.3 μg protein\n weight, 50.0 μg DNA+protein) in 36.2 μl 10mM Tris HCl, pH\n 7.5, 25mM NaCl, 1mM EDTA, 2mM DTT, 20% glycerol. Molarity =\n 6.90 μmolar. MW = 199997.8 Da.<br />\n <br />\n </span>\n </span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 12pt\" class=\"Apple-style-span\">\n <b>\n Nucleosome, Recombinant Human, H3K9cr dNuc Biotinylated\n Storage and Stability:</b\n >\n Stable for six months at -80°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.<br />\n <br />\n </span>\n </span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 12pt\" class=\"Apple-style-span\">\n <b>\n Nucleosome, Recombinant Human, H3K9cr dNuc Biotinylated\n Application Notes:</b\n >\n Nucleosome, Recombinant Human, H3K9cr are highly purified\n and are suitable for use as substrates in enzyme screening\n assays or for effector protein binding experiments.\n Nucleosome, Recombinant Human, H3K9cr from EpiCypher does\n not contain free DNA which could alter assayed\n activities.<br />\n <br />\n </span>\n </span>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <p>\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 12pt\" class=\"Apple-style-span\">\n <b> References:</b>\n <br />\n <br />\n </span>\n </span>\n <br />\n </p>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 12pt\" class=\"Apple-style-span\">\n View technical datasheet for this product.\n <a href=\"/content/documents/tds/16-0351.pdf\" target=\"_new\">\n <img\n src=\"/content/documents/tds/icon.png\"\n alt=\"16-0351 Datasheet\"\n width=\"30\"\n height=\"40\"\n />\n </a>\n </span>\n </span>\n </td>\n </tr>\n </tbody>\n </table>\n </td>\n <td>\n <table width=\"100%\" cellspacing=\"3\" cellpadding=\"3\">\n <tbody>\n <tr>\n <td>\n <a\n href=\"/content/images/products/nucleosomes/16-0351-WB-data.jpg\"\n target=\"_new\"\n >\n <img\n src=\"/content/images/products/nucleosomes/16-0351-WB-data.jpg\"\n alt=\"16-0351 Western Blot\"\n width=\"125\"\n height=\"325\"\n />\n </a>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 10pt\" class=\"Apple-style-span\">\n <strong> Western Blot Data:</strong>\n Western Analysis of Nucleosome, Recombinant Human, H3K9cr.\n Top Panel: Unmodified H3 (Lane 1) and H3K9cr containing\n nucleosomes (Lane 2) were probed with an anti-H3K9cr\n antibody and analyzed via ECL readout. Only the H3K9cr\n sample produced a detectable signal. Bottom Panel: Detail\n from Coomassie stained gel showing unmodified nucleosomes\n (Lane 1) and H3K9cr nucleosomes (Lane 2).<br />\n <strong> (Click image to enlarge) </strong>\n </span>\n </span>\n </td>\n </tr>\n </tbody>\n </table>\n <table width=\"100%\" cellspacing=\"3\" cellpadding=\"3\">\n <tbody>\n <tr>\n <td>\n <a\n href=\"/content/images/products/nucleosomes/16-0351-MS-data.jpg\"\n target=\"_new\"\n >\n <img\n src=\"/content/images/products/nucleosomes/16-0351-MS-data.jpg\"\n alt=\"16-0351 MS Data\"\n width=\"125\"\n height=\"125\"\n />\n </a>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 10pt\" class=\"Apple-style-span\">\n <strong> Mass Spec Data: </strong>\n Synthetic H3K9cr protein analyzed by high resolution mass\n spectrometry. Expected mass = 15,291.8 Da. Determined mass =\n 15,292.8Da.\n </span>\n </span>\n </td>\n </tr>\n </tbody>\n </table>\n <table width=\"100%\" cellspacing=\"3\" cellpadding=\"3\">\n <tbody>\n <tr>\n <td>\n <a\n href=\"/content/images/products/nucleosomes/16-0351-protein-gel.jpg\"\n target=\"_new\"\n >\n <img\n src=\"/content/images/products/nucleosomes/16-0351-protein-gel.jpg\"\n alt=\"16-0351 Protein Gel Data\"\n width=\"125\"\n height=\"325\"\n />\n </a>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 10pt\" class=\"Apple-style-span\">\n <strong> Protein Gel Data:</strong>\n Coomassie stained PAGE gel of proteins in Nucleosome,\n Recombinant Human, H3K9cr (1 μg) to demonstrate the purity\n of the histones in the preparation. Sizes of molecular\n weight markers and positions of the core histones (H2A, H2B,\n H3K9cr and H4) are indicated.<br />\n <strong> (Click image to enlarge) </strong>\n </span>\n </span>\n </td>\n </tr>\n </tbody>\n </table>\n <table width=\"100%\" cellspacing=\"3\" cellpadding=\"3\">\n <tbody>\n <tr>\n <td>\n <a\n href=\"/content/images/products/nucleosomes/16-0351-DNA-gel.jpg\"\n target=\"_new\"\n >\n <img\n src=\"/content/images/products/nucleosomes/16-0351-DNA-gel.jpg\"\n alt=\"16-0351 DNA Gel Data\"\n width=\"125\"\n height=\"325\"\n />\n </a>\n </td>\n </tr>\n <tr>\n <td valign=\"top\">\n <span style=\"font-family: Arial\" class=\"Apple-style-span\">\n <span style=\"font-size: 10pt\" class=\"Apple-style-span\">\n <strong> DNA Gel Data:</strong>\n Nucleosome, Recombinant Human, H3K9cr via native PAGE and\n stained with ethidium bromide to visualize DNA.\n <strong> Lane 1:</strong> Free DNA extracted from\n nucleosomes (100 ng). <strong> Lane 2:</strong> Intact\n nucleosomes (400 ng).<br />\n <strong> (Click image to enlarge) </strong>\n </span>\n </span>\n </td>\n </tr>\n </tbody>\n </table>\n </td>\n </tr>\n </tbody>\n</table>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Nucleosome, Recombinant Human, H3K9cr dNuc","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":496,"sku":"16-1314","name":"Nucleosome, Recombinant Human, H3K9ac dNuc","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k9ac-dnuc","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/496/338/Recombinant_nucleosome__97390.1516390304.png?c=2","alt":"Nucleosome, 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Pack Size: 50 μg
