SKU: 16-0367
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k79me1-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":612,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Nucleosome, Recombinant Human, H3K79me1 dNuc, Biotinylated","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/612/561/Recombinant_nucleosome__92684.1538135198.png?c=2","alt":"Nucleosome, Recombinant Human, H3K79me1 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=612","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"410","name":"Pack Size","value":"50 μg"}],"sku":"16-0367","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K79me1 dNuc, Biotinylated Description:</b> Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A,\nH2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains monomethyl-lysine at position 79. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has\nhigh affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K79me1</b></span></span> dNuc Biotinylated Formulation:</b> Nucleosome, Recombinant Human, H3K79me1 (27.3 μg protein weight, 50 μg total weight) in μl of 10 mM Tris\nHCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 199,885.9 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K79me1 dNuc,\nBiotinylated Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For\nbest results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K79me1 dNuc,\nBiotinylated Application Notes:</b> Nucleosome, Recombinant Human, H3K79me1 dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K79me1 dNucs from EpiCypher does not\ncontain free DNA which could alter assayed activities.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br />Lowary PT and J Widom (1998). J Mol Biol 276: 19-42. <br />\nLuger K et al (1999). Methods Mol Biol 119: 1-16.<br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0367.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0367 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0367-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0367-WB-data.jpg\" alt=\"16-0367 Western Blot\" width=\"125\" height=\"325\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong>Western Analysis of Nucleosome,\nRecombinant Human, H3K79me1. Top Panel: Unmodified H3 (Lane 1) and H3K79me1 containing nucleosomes (Lane 2) were probed with an anti-H3K79me1 antibody and analyzed via ECL readout. Only the H3K79me1 sample produced a detectable\nsignal. Bottom Panel: Detailfrom Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H3K79me1 nucleosomes (Lane 2).<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0367-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0367-MS-data.jpg\" alt=\"16-0367 MS Data\" width=\"125\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong>H3K79me1 protein analyzed by high\nresolution mass spectrometry. Expected mass = 15,285.9 Da. Determined mass = 15,286.6 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0367-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0367-protein-gel.jpg\" alt=\"16-0367 Protein Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in\nNucleosome, Recombinant Human, H3K79me1 (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K79me1 and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0367-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0367-DNA-gel.jpg\" alt=\"16-0367 DNA Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H3K79me1 run on a native PAGE gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (100 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).<br /> <strong>(Click image to enlarge) 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Pack Size: 50 μg
