SKU: 16-0335
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k4me3-k9-14-18ac-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":596,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Nucleosome, Recombinant Human, H3K4me3, K9,14,18ac dNuc, biotinylated","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/596/545/Recombinant_nucleosome__16505.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=596","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"394","name":"Pack Size","value":"50 μg"}],"sku":"16-0335","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac\ndNuc, Biotinylated Description:</b> Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2- Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains trimethyl-lysine at position 4, and acetyl-lysine at positions 9, 14, and 18. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.\n* H3K4me3,K9,14,18ac also has a Cys to Ala substitution at\nposition 110.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac</b></span></span> dNuc Biotinylated Formulation:</b> Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac (27.3 μg protein weight, 50 μg total weight) in\n10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.\nMW = 200,644 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac dNuc Biotinylated Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For\nbest results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac dNuc Biotinylated Application Notes:</b> Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0335.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0335 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0335-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0335-WB-data.jpg\" alt=\"16-0335 Western Blot\" width=\"125\" height=\"325\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong>Western Analysis of Nucleosome, Recombinant\nHuman, H3K4me3,K9,14,18ac. Top Panel: Unmodified H3 (Lane 1) and H3K4me3,K9,14,18ac containing nucleosomes (Lanes 2-5) were probed with an anti-H3K4me3, anti-H3K9ac, anti-H3K14ac, and anti-H3K18ac antibodies and analyzed via ECL readout. Only the H3K4me3,K9,14,18ac sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H3K4me3,K9,14,18ac nucleosomes (Lanes 2-5).<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0335-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0335-MS-data.jpg\" alt=\"16-0335 MS Data\" width=\"125\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong>H3K4me3,K9,14,18ac protein analyzed by high resolution mass spectrometry. Expected mass = 15,389.8 Da. Determined mass = 15,391.5 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0335-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0335-protein-gel.jpg\" alt=\"16-0335 Protein Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in\nNucleosome, Recombinant Human, H3K4me3,K9,14,18ac (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K4me3,K9,14,18ac and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0335-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0335-DNA-gel.jpg\" alt=\"16-0335 DNA Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H3K4me3,K9,14,18ac run on a native PAGE gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> </td></tr></tbody></table>\n\n\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Nucleosome, Recombinant Human, H3K4me3, K9,14,18ac dNuc, Biotinylated","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":296,"sku":null,"name":"Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k4me3-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)","Nucleosomes/Modified Designer Nucleosomes (dNucs™)/dNuc - Smaller Pack Size Available"],"summary":"\n \n \n Species: Human\n \n \n Source: E. coli & synthetic DNA\n \n \n \n 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Pack Size: 50 μg
