SKU: 16-0402
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k4-k9me3-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":920,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant nucleosome with trimethylated histone H3 at lysines 4 and 9 offers a defined substrate for chromatin research and drug discovery. ","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/920/1023/dNucs__31179.1569012501__00279.1652467571.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4,K9me3 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=920","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1088","name":"Pack Size","value":"50 µg"}],"sku":"16-0402","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong><i>E. coli</i> & synthetic DNA\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Tag: </strong>Biotinylated\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Molecular Weight: </strong>199,814.1 Da\n </li>\n </ul>\n </div>\n <div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n Recombinant mononucleosomes (H3K4,K9me3) consist of 147 base\n pairs of DNA wrapped around an octamer core of histone\n proteins (two each of histones H2A, H2B, H3.2 and H4). The 147\n bp 601 sequence, identified by Lowary and Widom [1], has high\n affinity for histone octamers and is useful for nucleosome\n assembly. H3K4,K9me3 dNuc contains trimethylated-lysines at\n positions 4 and 9 on histone H3.2. H3K4,K9me3 has a Cys to Ala\n substitution at position 110. The DNA contains a 5’ biotin-TEG\n group.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure1.jpeg?t=1700205913\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0402-dna-gel-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure1.jpeg?t=1700205913\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Protein gel data </strong><br />\n Coomassie stained SDS-PAGE gel of proteins in\n H3K4,K9me3 dNuc (1 µg) demonstrates the purity of\n histones in the preparation. Sizes of molecular weight\n markers and positions of the core histones (H2A, H2B,\n H3K4,K9me3, and H4) are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure-2.jpeg?t=1700205914\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0402-mass-spec-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure-2.jpeg?t=1700205914\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Mass spec data </strong><br />\n Semi-synthetic H3K4,K9me3 histone analyzed by high\n resolution mass spectrometry. Expected mass = 15,308.8\n Da. Determined mass = 15,309.3 Da.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure-3.jpeg?t=1700205915\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img\n alt=\"16-0402-protein-gel-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure-3.jpeg?t=1700205915\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: DNA gel data </strong><br />\n H3K4,K9me3 dNuc resolved via native PAGE and stained\n with ethidium bromide to visualize DNA.\n <strong>Lane 1</strong>: Free DNA (EpiCypher\n <a\n href=\"https://www.epicypher.com/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\"\n target=\"_blank\"\n >18-0005</a\n >; 100 ng). <strong>Lane 2</strong>: Intact H3K4,K9me3\n nucleosomes (400 ng).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"16-0402-dna-gel-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure1.jpeg?t=1700205913\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"16-0402-mass-spec-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure-2.jpeg?t=1700205914\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img\n alt=\"16-0402-protein-gel-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0402-figure-3.jpeg?t=1700205915\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\nStable for six months at -80°C from date of receipt. For\n best results, aliquot and avoid freeze/thaws\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT,\n 20% glycerol (27.2 µg protein, 50 µg DNA + protein)\n\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n H3K4,K9me3 dNuc is highly purified and suitable for a variety\n of applications, including use as a substrate in enzyme\n assays, high-throughput screening and inhibitor testing,\n chromatin binding studies, protein-protein interaction assays,\n structural studies, and in effector protein binding\n experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Uniprot ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin-bottom: 0\">\n H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a,\n H2A/m)\n </p>\n <p style=\"margin-bottom: 0\">\n H2B - O60814 (alt. names: H2B K, HIRA-interacting\n protein 1)\n </p>\n <p style=\"margin-bottom: 0\">H3.2 - Q71DI3</p>\n <p style=\"margin-bottom: 0\">H4 - P62805</p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n target=\"_blank\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n >9514715</a\n ><br />\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0402.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Technical Datasheet</span\n >\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H3K4,K9me3 Recombinant Nucleosome (dNuc)","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":921,"sku":"16-0403","name":"Nucleosome, Recombinant Human, H3K4,K27me3 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k4-k27me3-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":"\n \n \n \n Description\n \n \n Mononucleosomes assembled from recombina","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/921/1025/dNucs__31179.1569012501__62666.1652468319.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4,K27me3 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/921/1025/dNucs__31179.1569012501__62666.1652468319.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4,K27me3 dNuc, Biotinylated"}],"date_added":"13th May 2022","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1089,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=921","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=921"},{"id":504,"sku":null,"name":"Nucleosome, Recombinant Human, H3K27ac dNuc Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k27ac-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)","Nucleosomes/Modified Designer Nucleosomes (dNucs™)/dNuc - Smaller Pack Size Available"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/504/355/Recombinant_nucleosome__81635.1515790478.1280.1280__51020.1520394110.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27ac dNuc Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/504/355/Recombinant_nucleosome__81635.1515790478.1280.1280__51020.1520394110.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27ac dNuc Biotinylated"}],"date_added":"1st Dec 2017","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":831,"name":"Pack Size","value":"50 µg"},{"id":832,"name":"Pack Size","value":"20 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$295.00","value":295},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"}},"tax_label":"Sales 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2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":200,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=367","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=367"},{"id":610,"sku":"16-0364","name":"Nucleosome, Recombinant Human, H3K23ac dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k23ac-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" 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Pack Size: 50 µg
