SKU: 16-0374
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k4-9-14-18ac-h4k5-8-12-16ac-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":668,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/668/628/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__42896.1556669815.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=668","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"467","name":"Pack Size","value":"50 μg"}],"sku":"16-0374","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac dNuc, Biotinylated Description:</b> Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A,\nH2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains acetylated lysine at positions 4, 9, 14, and 18 and H4 (created by a proprietary synthetic method) contains acetylated lysine at positions 5, 8, 12, and 16. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has\nhigh affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group. *H3K4,9,14,18ac has a Cys to Ala substitution at position 110.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac</b></span></span> dNuc Biotinylated Formulation:</b> Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac (27.3 μg protein weight, 50 μg DNA+protein) in μl 10 mM Tris HCl,\npH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 200,543 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac dNuc,\nBiotinylated Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For\nbest results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac dNuc,\nBiotinylated Application Notes:</b> Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac dNucs from EpiCypher does not\ncontain free DNA which could alter assayed activities.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:<br /></b> Lowary PT and J Widom (1998). J Mol Biol 276: 19-42.<br />\nLuger K et al (1999). Methods Mol Biol 119: 1-16.<br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0374.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0374 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0374-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0374-DNA-gel.jpg\" alt=\"16-0374 DNA gel\" width=\"125\" height=\"325\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong>Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac run on a native PAGE gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (100 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng)<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0374-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0374-protein-gel.jpg\" alt=\"16-0374 Protein Gel\" width=\"125\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in\nNucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K4,9,14,18ac and H4K5,8,12,16ac) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody>\n\t\n\t<tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0374-H3-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0374-H3-MS-data.jpg\" alt=\"16-0374 H3 MS Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> H3K4,9,14,18ac protein analyzed by high resolution mass spectrometry. Expected mass = 15,389.8 Da. Determined mass = 15,391.4 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr>\n\n\n\n<tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0374-H4-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0374-H4-MS-data.jpg\" alt=\"16-0374 H4 MS Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> H4K5,8,12,16ac protein analyzed by high resolution mass spectrometry. Expected mass = 11,446.0 Da. Determined mass = 11,446.0 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr>\n\n\n\n\n</tbody></table></td></tr>\n\n\n\n</tbody></table> </td></tr></tbody></table>\n\n\n\n\n\n\n\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":597,"sku":"16-0336","name":"Nucleosome, Recombinant Human, H3K4,9,14,18ac dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k4-9-14-18ac-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" 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Pack Size: 50 μg
