SKU: 16-0383
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k27cr-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":676,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/676/635/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__42896.1556669781.1280.1280__07755.1556670109.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K27cr dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=676","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"661","name":"Pack Size","value":"50 µg"}],"sku":"16-0383","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K27cr dNuc, Biotinylated Description:</b> Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A,\nH2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains crotonyl-lysine at position 27. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has\nhigh affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group. *H3K27cr also has a Cys to Ala substitution at position 110.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3K27cr</b></span></span> dNuc Biotinylated Formulation:</b> Nucleosome, Recombinant Human, H3K27cr (27.3 μg protein weight, 50 μg DNA+protein) in μl 10 mM Tris HCl,\npH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 199,997.8 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K27cr dNuc,\nBiotinylated Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For\nbest results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3K27cr dNuc,\nBiotinylated Application Notes:</b> Nucleosome, Recombinant Human, H3K27cr dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3K27cr dNucs from EpiCypher does not\ncontain free DNA which could alter assayed activities.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:<br /></b> Lowary PT and J Widom (1998). J Mol Biol 276: 19-42.<br />\nLuger K et al (1999). Methods Mol Biol 119: 1-16.<br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0383.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0383 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0383-Reader-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0383-Reader-data.jpg\" alt=\"16-0383 Reader Data\" width=\"125\" height=\"325\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Chromatin Reader Binding Data:</strong> AF9 YEATS domain exhibits preferential binding to crotonyl lysine over acetyl-lysine. AlphaScreen interrogation of AF9 YEATS Domain (1 uM, EpiCypher Catalog No: 15-0071) binding to biotinylated unmodified (rNuc, EpiCypher Catalog No. 16-0006), H3K27ac (EpiCypher Catalog No. 16-0365), and H3K27cr nucleosomes (10 nM) shows an increased binding to H3K27cr. Nucleosomes and AF9 were incubated for 30 minutes followed by a 60 minute incubation with streptavidin donor beads and nickel-chelate acceptor beads.<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0383-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0383-MS-data.jpg\" alt=\"16-0383 MS Data\" width=\"125\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong>H3K27cr protein analyzed by high resolution mass spectrometry. Expected mass = 15,291.8 Da. Determined mass = 15,292.54 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0383-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0383-protein-gel.jpg\" alt=\"16-0383 Protein Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in\nNucleosome, Recombinant Human, H3K27cr (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K27cr, and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0383-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0383-DNA-gel.jpg\" alt=\"16-0383 DNA Gel Data\" width=\"125\" height=\"325\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H3K27cr run on a native PAGE gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (100 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> </td></tr></tbody></table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":605,"sku":"16-0358","name":"Nucleosome, Recombinant Human, H4R3me2s dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h4r3me2s-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/605/554/Recombinant_nucleosome__90101.1538135197.png?c=2","alt":"Nucleosome, Recombinant Human, H4R3me2s dNuc, 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Pack Size: 50 µg
