Nucleosome, Recombinant Human, H3K18ub1 dNuc, Biotinylated
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k18ub1-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":882,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant nucleosome with monoubiquitinylated histone H3 at lysine 18 offers a defined substrate for chromatin research and drug discovery. ","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/882/1024/dNucs__31179.1569012501__21037.1652467635.png?c=2","alt":"Nucleosome, Recombinant Human, H3K18ub1 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=882","shipping":{"calculated":true},"num_reviews":0,"weight":"0.00 LBS","custom_fields":[{"id":"1090","name":"Pack Size","value":"25 µg"},{"id":"1091","name":"Internal Comment","value":"Lot 2 in New Lot Rack"}],"sku":"16-0401","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <p>\n Mononucleosomes assembled from recombinant human histones expressed\n in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4;\n accession numbers: H2A-P20671; H2B-O60814; H3.1-P68431; H4-P62805)\n wrapped by 147 base pairs of 601 positioning sequence DNA. Histone\n H3 (created by a proprietary semi-synthetic method) contains\n ubiquitin-lysine at position 18. The nucleosome is the basic subunit\n of chromatin. The 147 bp 601 sequence, identified by Lowary and\n Widom [1], has high affinity for histone octamers and is useful for\n nucleosome assembly. The DNA contains a 5’ biotin-TEG group.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"\n image-picker__main-content_active image-picker__main-content\n \"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0400-dna-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0400-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0400-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA Gel Data </strong><br />\n H3K18ub1 dNuc resolved via native PAGE and stained with\n ethidium bromide to visualize DNA.\n <strong>Lane 1:</strong> Free DNA (EpiCypher\n <a\n href=\"/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\"\n >18-0005</a\n >; 100 ng). <strong>Lane 2:</strong> Intact H3K18ub1\n nucleosomes (400 ng).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0401-mass-spec-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0401-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-0401-mass-spec-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Mass Spec Data </strong><br />\n Semi-synthetic H3K18ub1 histone analyzed by high resolution\n mass spectrometry. Expected mass = 23,771.2 Da. Determined\n mass = 23,771.16 Da.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0401-protein-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img loading=\"lazy\"\n alt=\"16-0401-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0401-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Protein Gel Data </strong><br />\n Coomassie stained PAGE gel of proteins in H3K18ub1 dNuc (1\n µg) demonstrates the purity of histones in the preparation.\n Sizes of molecular weight markers and positions of the core\n histones (H2A, H2B, H3K18ub1, and H4) are indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"16-0401-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0401-dna-gel-data.jpeg\"\n width=\"200\"\n class=\"\n image-picker__side-image image-picker__side-image_active\n \"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"16-0401-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-0401-mass-spec-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"16-0401-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0401-protein-gel-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n H3K18ub1 dNuc (14.4 µg protein weight, 25 µg DNA + protein) in\n 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20%\n glycerol. MW = 216,857.3 Da.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six (6) months at -80°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p>\n H3K18ub1 dNuc is highly purified and suitable for a variety of\n applications, including use as a substrate in enzymatic assays or\n for effector protein binding experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n target=\"_blank\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n >9514715</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0401.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$525.00","value":525,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H3K18ub1 Recombinant Nucleosome (dNuc)","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":504,"sku":null,"name":"Nucleosome, Recombinant Human, H3K27ac dNuc Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k27ac-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)","Nucleosomes/Modified Designer Nucleosomes (dNucs™)/dNuc - Smaller Pack Size Available"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/504/355/Recombinant_nucleosome__81635.1515790478.1280.1280__51020.1520394110.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27ac dNuc Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/504/355/Recombinant_nucleosome__81635.1515790478.1280.1280__51020.1520394110.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27ac dNuc Biotinylated"}],"date_added":"1st Dec 2017","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":831,"name":"Pack Size","value":"50 µg"},{"id":832,"name":"Pack Size","value":"20 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$295.00","value":295},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=504"},{"id":367,"sku":"16-0331","name":"Nucleosome, Recombinant Human, H4K20me1 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h4k20me1-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/367/311/Recombinant_nucleosome__95480.1582226113.png?c=2","alt":"Nucleosome, Recombinant Human, H4K20me1 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/367/311/Recombinant_nucleosome__95480.1582226113.png?c=2","alt":"Nucleosome, Recombinant Human, H4K20me1 dNuc, Biotinylated"}],"date_added":"26th May 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":200,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=367","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=367"},{"id":610,"sku":"16-0364","name":"Nucleosome, Recombinant Human, H3K23ac dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k23ac-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/610/559/Recombinant_nucleosome__48872.1538135197.png?c=2","alt":"Nucleosome, Recombinant Human, H3K23ac dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/610/559/Recombinant_nucleosome__48872.1538135197.png?c=2","alt":"Nucleosome, Recombinant Human, H3K23ac dNuc, Biotinylated"}],"date_added":"28th Aug 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":408,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=610","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=610"},{"id":600,"sku":"16-0342","name":"Nucleosome, Recombinant Human, H3K4ac dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k4ac-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/600/549/Recombinant_nucleosome__97086.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4ac dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/600/549/Recombinant_nucleosome__97086.1538135202.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4ac dNuc, Biotinylated"}],"date_added":"28th Aug 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":398,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=600","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=600"},{"id":314,"sku":null,"name":"Nucleosome, Recombinant Human, H3K4me1 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k4me1-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)","Nucleosomes/Modified Designer Nucleosomes (dNucs™)/dNuc - Smaller Pack Size Available"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/314/294/dNucs__31179.1569012501.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me1 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/314/294/dNucs__31179.1569012501.png?c=2","alt":"Nucleosome, Recombinant Human, H3K4me1 dNuc, Biotinylated"}],"date_added":"5th Aug 2016","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1047,"name":"Internal Comment","value":"Has Bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$295.00","value":295},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=314"},{"id":376,"sku":"16-0339","name":"Nucleosome, Recombinant Human, H3K27me2 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k27me2-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/376/302/Recombinant_nucleosome__59688.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27me2 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/376/302/Recombinant_nucleosome__59688.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27me2 dNuc, Biotinylated"}],"date_added":"1st Aug 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":204,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=376","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=376"},{"id":607,"sku":"16-0360","name":"Nucleosome, Recombinant Human, H2AR3me2a dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h2ar3me2a-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/607/556/Recombinant_nucleosome__55974.1538135197.png?c=2","alt":"Nucleosome, Recombinant Human, H2AR3me2a dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/607/556/Recombinant_nucleosome__55974.1538135197.png?c=2","alt":"Nucleosome, Recombinant Human, H2AR3me2a dNuc, Biotinylated"}],"date_added":"28th Aug 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":405,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=607","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=607"},{"id":606,"sku":"16-0359","name":"Nucleosome, Recombinant Human, H2AR3me1 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h2ar3me1-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/606/555/Recombinant_nucleosome__54380.1538135197.png?c=2","alt":"Nucleosome, Recombinant Human, H2AR3me1 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/606/555/Recombinant_nucleosome__54380.1538135197.png?c=2","alt":"Nucleosome, Recombinant Human, H2AR3me1 dNuc, Biotinylated"}],"date_added":"28th Aug 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":811,"name":"Pack Size","value":"50 μg"},{"id":812,"name":"Internal Comment","value":"extra in bottom right of Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=606","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=606"},{"id":677,"sku":"16-0384","name":"Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k27bu-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/677/634/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__42896.1556669781.1280.1280__94005.1556670077.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/677/634/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__42896.1556669781.1280.1280__94005.1556670077.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3K27bu dNuc, Biotinylated"}],"date_added":"30th Apr 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":466,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=677","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=677"}],"shipping_messages":[],"rating":0,"meta_keywords":"H3K18ub1, H3K18ub, H3K18ubiquitin, Recombinant Nucleosome, human nucleosome, histone H3 lysine 18 ubiquitin, monoubiquitin, dNuc, mononucleosome","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H3K18ub1 dNuc, Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/882/1024/dNucs__31179.1569012501__21037.1652467635.png?c=2","alt":"Nucleosome, Recombinant Human, H3K18ub1 dNuc, Biotinylated"}]} Pack Size: 25 µg
Description
Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P20671; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains ubiquitin-lysine at position 18. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group.
Validation Data
Figure 1: DNA Gel Data
H3K18ub1 dNuc resolved via native PAGE and stained with
ethidium bromide to visualize DNA.
Lane 1: Free DNA (EpiCypher
18-0005; 100 ng). Lane 2: Intact H3K18ub1
nucleosomes (400 ng).
Figure 2: Mass Spec Data
Semi-synthetic H3K18ub1 histone analyzed by high resolution
mass spectrometry. Expected mass = 23,771.2 Da. Determined
mass = 23,771.16 Da.
Technical Information
Application Notes
H3K18ub1 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.