Nucleosome, Recombinant Human, H2AK15ub1 dNuc, Biotinylated
{"url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h2ak15ub1-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":880,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant nucleosome with monoubiquitinylated histone H2A at lysine 15 offers a defined substrate for chromatin research and drug discovery. ","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/880/908/1591818618.1280.1280__68061.1607995447.1280.1280__45790.1614276469__05512.1627058224.190.250__26703.1629128888.jpg?c=2","alt":"Nucleosome, Recombinant Human, H2AK15ub1 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=880","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"940","name":"Pack Size","value":"25 µg"}],"sku":"16-0399","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <p>\n Mononucleosomes assembled from recombinant human histones expressed\n in E. coli (two each of histones H2A, H2B, H3 and H4; accession\n numbers: H2A-P20671; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by\n 147 base pairs of 601 positioning sequence DNA. Histone H2A (created\n by a proprietary semi-synthetic method) contains ubiquitin-lysine at\n position 15. The nucleosome is the basic subunit of chromatin. The\n 147 bp 601 sequence, identified by Lowary and Widom [1], has high\n affinity for histone octamers and is useful for nucleosome assembly.\n The DNA contains a 5’ biotin-TEG group.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"\n image-picker__main-content_active image-picker__main-content\n \"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0399-dna-gel-data.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0399-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0399-dna-gel-data.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA Gel Data </strong><br />\n H2AK15ub1 dNuc resolved via native PAGE and stained with\n ethidium bromide to visualize DNA.\n <strong>Lane 1:</strong> Free DNA (EpiCypher\n <a\n href=\"/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\"\n >18-0005</a\n >; 100 ng). <strong>Lane 2:</strong> Intact H2AK15ub1\n nucleosomes (400 ng).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0399-mass-spec-data.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"16-0399-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-0399-mass-spec-data.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Mass Spec Data </strong><br />\n Semi-synthetic H2AK15ub1 histone analyzed by high resolution\n mass spectrometry. Expected mass = 22,522.12 Da. Determined\n mass = 22,522.51 Da.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0399-protein-gel-data.jpg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n >\n <img loading=\"lazy\"\n alt=\"16-0399-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0399-protein-gel-data.jpg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Protein Gel Data </strong><br />\n Coomassie stained PAGE gel of proteins in H2AK15ub1 dNuc (1\n µg) demonstrates the purity of histones in the preparation.\n Sizes of molecular weight markers and positions of the core\n histones (H2AK15ub1, H2B, H3, and H4) are indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"16-0399-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0399-dna-gel-data.jpg\"\n width=\"200\"\n class=\"\n image-picker__side-image image-picker__side-image_active\n \"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"16-0399-mass-spec-data\"\n src=\"/content/images/products/nucleosomes/16-0399-mass-spec-data.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n <img loading=\"lazy\"\n alt=\"16-0399-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0399-protein-gel-data.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n H2AK15ub1 dNuc (14.4 µg protein weight, 25 µg DNA + protein) in\n 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20%\n glycerol. MW = 216,895.8 Da.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six (6) months at -80°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p>\n H2AK15ub1 dNuc is highly purified and suitable for a variety of\n applications, including use as a substrate in enzymatic assays or\n for effector protein binding experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n target=\"_blank\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n >9514715</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0399.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$525.00","value":525,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H2AK15ub1 Recombinant Nucleosome (dNuc)","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":614,"sku":"16-0369","name":"Nucleosome, Recombinant Human, H3K79me3 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k79me3-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":"\n \n \n Species: Human\n \n \n Source: E. coli & synthetic DNA\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/614/563/Recombinant_nucleosome__08394.1538135198.png?c=2","alt":"Nucleosome, Recombinant Human, H3K79me3 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/614/563/Recombinant_nucleosome__08394.1538135198.png?c=2","alt":"Nucleosome, Recombinant Human, H3K79me3 dNuc, Biotinylated"}],"date_added":"28th Aug 2018","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":856,"name":"Pack Size","value":"50 μg"},{"id":857,"name":"Internal Comment","value":"has bulk, not included in count"},{"id":858,"name":"Internal Comment","value":"There are 21 units. but this is zero'd out do to issue with nuc."}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=614","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=614"},{"id":381,"sku":"16-0341","name":"Nucleosome, Recombinant Human, H3R2me2a dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3r2me2a-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/381/321/Recombinant_nucleosome__29081.1582226102.png?c=2","alt":"Nucleosome, Recombinant Human, H3R2me2a dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/381/321/Recombinant_nucleosome__29081.1582226102.png?c=2","alt":"Nucleosome, Recombinant Human, H3R2me2a dNuc, Biotinylated"}],"date_added":"1st Aug 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1066,"name":"Pack Size","value":"50 μg"},{"id":1067,"name":"Internal Comment","value":"Has Bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=381","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=381"},{"id":669,"sku":"16-0375","name":"Nucleosome, Recombinant Human, H3R17me2a dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3r17me2a-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/669/932/nucleosome-recombinant-human-h3r17me2a-dnuc-biotinylated__15451.1645734523.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3R17me2a dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/669/932/nucleosome-recombinant-human-h3r17me2a-dnuc-biotinylated__15451.1645734523.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3R17me2a dNuc, Biotinylated"}],"date_added":"30th Apr 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":570,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=669","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=669"},{"id":675,"sku":"16-0382","name":"Nucleosome, Recombinant Human, H3R17me1 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3r17me1-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/675/944/nucleosome-recombinant-human-h3r17me1-dnuc-biotinylated__41335.1645734535.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3R17me1 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/675/944/nucleosome-recombinant-human-h3r17me1-dnuc-biotinylated__41335.1645734535.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3R17me1 dNuc, Biotinylated"}],"date_added":"30th Apr 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":569,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=675","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=675"},{"id":682,"sku":"16-0389","name":"Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3-3s31phos-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":"\n \n \n Species: Human\n \n \n Source: E. coli & synthetic DNA\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/682/629/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__42896.1556669781.1280.1280__28948.1556669839.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/682/629/Recombinant_nucleosome__81635.1515790478.1280.1280__52955.1520379878.1280.1280__81555.1539965782.1280.1280__22682.1556128764.1280.1280__42896.1556669781.1280.1280__28948.1556669839.jpg?c=2","alt":"Nucleosome, Recombinant Human, H3.3S31phos dNuc, Biotinylated"}],"date_added":"30th Apr 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":566,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=682","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=682"}],"shipping_messages":[],"rating":0,"meta_keywords":"H2AK15ub1, H2AK15ub, H2AK15ubiquitin, Recombinant Nucleosome, human nucleosome, histone H2A lysine 15 ubiquitin, monoubiquitin, dNuc, mononucleosome","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H2AK15ub1 dNuc, Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/880/908/1591818618.1280.1280__68061.1607995447.1280.1280__45790.1614276469__05512.1627058224.190.250__26703.1629128888.jpg?c=2","alt":"Nucleosome, Recombinant Human, H2AK15ub1 dNuc, Biotinylated"}]} Pack Size: 25 µg
Description
Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P20671; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H2A (created by a proprietary semi-synthetic method) contains ubiquitin-lysine at position 15. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group.
Validation Data
Figure 1: DNA Gel Data
H2AK15ub1 dNuc resolved via native PAGE and stained with
ethidium bromide to visualize DNA.
Lane 1: Free DNA (EpiCypher
18-0005; 100 ng). Lane 2: Intact H2AK15ub1
nucleosomes (400 ng).
Figure 2: Mass Spec Data
Semi-synthetic H2AK15ub1 histone analyzed by high resolution
mass spectrometry. Expected mass = 22,522.12 Da. Determined
mass = 22,522.51 Da.
Technical Information
Application Notes
H2AK15ub1 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.