EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated

$565.00

SKU: 16-4111
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":470,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"EpiDyne Nucleosome Substrate for Chromatin Remodeling Assays and Inhibitor Screening","category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/470/805/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__98781.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=470","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1055","name":"Pack Size","value":"50 μg"},{"id":"1056","name":"Internal Comment","value":"Bulk in LG"}],"sku":"16-4111","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody>\n\n<tr valign=\"top\"> \n\n<td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> \n\n\n\n<tr> <td valign=\"top\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 218 base pair DNA sequence that includes the Widom 601 sequence with an added GATC and a 5' biotin-TEG group. \n \n \n\n </td> \n</tr> <tr> \n \n \n \n \n \n \n \n \n \n \n <td valign=\"top\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Formulation: Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 243,380 Da. </td> \n </tr> \n \n \n \n \n \n \n \n \n \n <tr> <td valign=\"top\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. </td> </tr> <tr> <td valign=\"top\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Application Notes: This product is a substrate for chromatin remodeling enzymes (e.g. <a href=\"https://www.epicypher.com/acf-remodeling-enzyme-complex/\" target=\"_new\">ACF Remodeling Enzyme Complex</a>) using restriction enzymes to determine accessibility of GATC subsequent to chromatin remodeling. </td> </tr> <tr> <td valign=\"top\">References: \n </td> </tr> \n \n <tr> <td valign=\"top\"> View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-4111.pdf\" target=\"_blank\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-4111 Datasheet\" width=\"30\" height=\"40\" /></a> </td> </tr> \n \n <tr> <td valign=\"top\"> View Restiction Enzyme Assay application technical note for this product. <a href=\"https://www.epicypher.com/content/documents/technotes/EpiDyne_REA.pdf\" target=\"_blank\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-4111 Tech Note\" width=\"30\" height=\"40\" /></a> </td> </tr> \n \n \n </tbody> </table> </td> \n \n \n \n <td width=\"350px\"> <table style=\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> \n \n \n <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4111_DNA_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4111_DNA_gel_tds.jpg\" alt=\"16-4111 Protein Gel\" width=\"150\"/></a></td> </tr> <tr> <td align=\"left\" valign=\"top\">DNA Gel Data: Free DNA (DNA, 200 ng) and EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated (Nuc, 370 ng) run on an acrylamide gel and stained with ethidium bromide to visualize DNA. Note absence of free DNA in "Nuc", indicating all DNA complexed with histones.</td> \n </tr> \n \n \n <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4111_protein_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4111_protein_gel_tds.jpg\" alt=\"16-4111 DNA Gel\" width=\"200\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\">Protein Gel Data: Coomassie stained PAGE gel of proteins in EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated (2 µg) demonstrates the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated. </td> \n </tr> \n \n \n \n \n <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4111_remodel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4111_remodel_tds.jpg\" alt=\"16-4111 REA Assay\" width=\"250\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\">Nucleosome Remodeling Data: RSC/ATP-dependent chromatin remodeling reaction in the presence of DpnII restriction enzyme. EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated was incubated with RSC chromatin remodeling complex (10 nm) or without (No RSC) for up to two hours in the presence of 2 mM ATP (or No ATP, as indicated) and 50U of DpnII. Samples were quenched at specified intervals and run on 8% native PAGE gel and stained with ethidium bromide. The apperance of a band at ~200 base pairs (red arrow) indicates cleavage at GATC subsequent to chromatin re remodeling.</td> \n </tr> \n \n \n \n \n \n \n </tbody> </table> </td> </tr> </tbody> </table> </div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$565.00","value":565,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"EpiDyne Nucleosome Substrate ST601-GATC1, Biotinylated | For Chromatin Remodeling Assays and Inhibitor Screening","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":551,"sku":"15-1013","name":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","availability":"","rating":null,"brand":{"name":null},"category":[],"summary":"\n \n Product Discontinued - we 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nucleosome sliding, sliding, restriction enzyme assay, DAM methyltransferase assay, , chromatin remodeling, BRM, CHD, chromatin remodeling factors, SMARCA, , SMARCA4, SNF2L4, nucleosome positioning, SWR1, ISWI, BAF190 assay, BAF190 complex, BAF190 remodeler, BAF190A, BAF190A assay, BAF190A atpase, BAF190A complex, BAF190A remodeler, BAF190B, BAF190B assay, BAF190B atpase, BAF190B complex, BAF190B remodeler, BRG1 assay, BRG1 atpase, BRG1 complex, BRG1 remodeler, BRM assay, BRM atpase, BRM complex, BRM remodeler, CHD assay, CHD atpase, CHD complex, CHD remodeler, INO80, INO80 assay, INO80 atpase, INO80 complex, INO80 remodeler, ISWI assay, ISWI atpase, ISWI complex, ISWI remodeler, Mi-2, Mi-2 assay, Mi-2 atpase, Mi-2 complex, Mi-2 remodeler, NuRD, NuRD assay, NuRD atpase, NuRD complex, NuRD remodeler, SMARCA assay, SMARCA atpase, SMARCA complex, SMARCA remodeler, SMARCA4 assay, SMARCA4 atpase, SMARCA4 complex, SMARCA4 remodeler, SNF2A, SNF2A assay, SNF2A atpase, SNF2A complex, SNF2A 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Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/470/805/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__98781.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/470/804/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_no_background_RGB_AL_MP__77219.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated"}]} Pack Size: 50 μg

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 218 base pair DNA sequence that includes the Widom 601 sequence with an added GATC and a 5' biotin-TEG group.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Formulation: Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 243,380 Da.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Application Notes: This product is a substrate for chromatin remodeling enzymes (e.g. ACF Remodeling Enzyme Complex) using restriction enzymes to determine accessibility of GATC subsequent to chromatin remodeling.

References:

View technical datasheet for this product.

View Restiction Enzyme Assay application technical note for this product.

DNA Gel Data: Free DNA (DNA, 200 ng) and EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated (Nuc, 370 ng) run on an acrylamide gel and stained with ethidium bromide to visualize DNA. Note absence of free DNA in "Nuc", indicating all DNA complexed with histones.

Protein Gel Data: Coomassie stained PAGE gel of proteins in EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated (2 µg) demonstrates the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.

Nucleosome Remodeling Data: RSC/ATP-dependent chromatin remodeling reaction in the presence of DpnII restriction enzyme. EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated was incubated with RSC chromatin remodeling complex (10 nm) or without (No RSC) for up to two hours in the presence of 2 mM ATP (or No ATP, as indicated) and 50U of DpnII. Samples were quenched at specified intervals and run on 8% native PAGE gel and stained with ethidium bromide. The apperance of a band at ~200 base pairs (red arrow) indicates cleavage at GATC subsequent to chromatin re remodeling.

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