EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":347,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"EpiDyne Nucleosome Substrate for Chromatin Remodeling Assays and Inhibitor Screening","category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/347/803/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__47518.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=347","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1053","name":"Pack Size","value":"50 μg"},{"id":"1054","name":"Internal Comment","value":"no more bulk 3/31/22"}],"sku":"16-4101","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <p>\n Mononucleosomes assembled from recombinant human histones expressed\n in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4.\n Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805)\n wrapped by provided 217 base pair DNA sequence that includes the\n Widom 601 sequence with an added GATC.\n </p>\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a\n target=\"_blank\"\n style=\"color: #fff\"\n href=\"/resources/protocols/epidyne-guide\"\n >Want to learn more? Check out our EpiDyne® Guide >>\n </a>\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n style=\"display: block\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-4101_protein_gel_tds.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-4101 DNA Gel\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_DNA_gel_tds.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA Gel Data </strong><br />Free DNA\n (DNA, 200 ng) and EpiDyne Nucleosome Remodeling Assay\n Substrate ST601-GATC1 (Nuc, 370 ng) run on an acrylamide gel\n and stained with ethidium bromide to visualize DNA.\n <strong\n >Note absence of free DNA in "Nuc", indicating\n all DNA complexed with histones.</strong\n >\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"content/images/products/nucleosomes/16-4101_DNA_gel_tds.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-4101 Protein Gel\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_protein_gel_tds.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein Gel Data </strong><br />\n Coomassie stained PAGE gel of proteins in EpiDyne Nucleosome\n Remodeling Assay Substrate ST601-GATC1 (2 µg) demonstrates\n the purity of the histones in the preparation. Sizes of\n molecular weight markers and positions of the core histones\n (H2A, H2B, H3 and H4) are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"content/images/products/nucleosomes/16-4101_remodel_tds.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n alt=\"16-4101 Western Blot\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_remodel_tds.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Nucleosome Remodeling Data </strong><br />\n RSC/ATP-dependent chromatin remodeling reaction in the\n presence of DpnII restriction enzyme. EpiDyne Nucleosome\n Remodeling Assay Substrate ST601-GATC1 was incubated with\n RSC chromatin remodeling complex (10 nm) or without (No RSC)\n for up to two hours in the presence of 2 mM ATP (or No ATP,\n as indicated) and 50U of DpnII. Samples were quenched at\n specified intervals and run on 8% native PAGE gel and\n stained with ethidium bromide. The apperance of a band at\n ~200 base pairs (red arrow) indicates cleavage at GATC\n subsequent to chromatin remodeling.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"16-0009 DNA Gel\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_DNA_gel_tds.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n alt=\"16-0009 Protein Gel\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_protein_gel_tds.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n alt=\"16-4101 Western Blot\"\n src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4101_remodel_tds.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p>\n Purified recombinant mononucleosomes 1.70 mg/ml (DNA + protein\n weight) in 29.4 µl of 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM\n EDTA, 2 mM DTT, 20% glycerol. MW = 242,900 Da. Molarity = 7.00\n µM.\n </p>\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six (6) months at -80°C from date of receipt. For\n best results, aliquot and avoid multiple freeze/thaws.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This product is a substrate for chromatin remodeling assays (<em\n >e.g.</em\n >\n <a href=\"/acf-remodeling-enzyme-complex/\" target=\"_new\"\n >ACF Remodeling Enzyme Complex</a\n >) using restriction enzymes to determine accessibility of GATC\n subsequent to chromatin remodeling.\n </p>\n </div>\n </div>\n </div>\n<div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">DNA Sequence</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p style=\"line-break: anywhere;\">\n CTAGGAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGGTCG\n TAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCG\n CGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGT\n CAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGA\n TGGATGATGATGGATGAATAGATGGATGGATGAAGCTTTAGG\n </p>\n \n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Product References:</strong>\n <iframe\n class=\"airtable-embed\"\n src=\"https://airtable.com/embed/shrVZ2ecXmqA8RE03?backgroundColor=purple&viewControls=on\"\n frameborder=\"0\"\n onmousewheel=\"\"\n width=\"100%\"\n height=\"300\"\n style=\"background: transparent; border: 1px solid #ccc\"></iframe>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-4101.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/technotes/EpiDyne_REA.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Restriction Enzyme Assay application technical note for this\n product</span\n >\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$565.00","value":565,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"EpiDyne Nucleosome Substrate ST601-GATC1 | For Chromatin Remodeling Assays and Inhibitor Screening","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":551,"sku":"15-1013","name":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","availability":"","rating":null,"brand":{"name":null},"category":[],"summary":"\n \n Product Discontinued - we recommend SMARCA5 (15","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2","alt":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/551/597/Untitled__22497.1556128753.png?c=2","alt":"ACF Chromatin Remodeling Enzyme Complex *DISCONTINUED*"}],"date_added":"24th Jul 2018","pre_order":false,"show_cart_action":false,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":585,"name":"Pack Size","value":"100 reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=551"},{"id":386,"sku":"16-4201","name":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate","url":"https://www.epicypher.com/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":"\n \n \n \n Description\n \n \n This product provides a h","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/386/276/2_EpiDyne__20008.1569012494.png?c=2","alt":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/386/276/2_EpiDyne__20008.1569012494.png?c=2","alt":"EpiDyne-FRET Nucleosome Remodeling Assay Substrate"}],"date_added":"28th Aug 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1138,"name":"Pack Size","value":"50 µg"},{"id":1139,"name":"Internal Comment","value":"Bulk in Bulk Nuc Box 2 in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.00 LBS","value":0},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=386","price":{"without_tax":{"currency":"USD","formatted":"$595.00","value":595},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=386"},{"id":470,"sku":"16-4111","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":" \n\n \n\n \n\n\n\n EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Description: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/470/805/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__98781.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, 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nucleosome sliding, sliding, restriction enzyme assay, DAM methyltransferase assay, , chromatin remodeling, BRM, CHD, chromatin remodeling factors, SMARCA, , SMARCA4, SNF2L4, nucleosome positioning, SWR1, ISWI, BAF190 assay, BAF190 complex, BAF190 remodeler, BAF190A, BAF190A assay, BAF190A atpase, BAF190A complex, BAF190A remodeler, BAF190B, BAF190B assay, BAF190B atpase, BAF190B complex, BAF190B remodeler, BRG1 assay, BRG1 atpase, BRG1 complex, BRG1 remodeler, BRM assay, BRM atpase, BRM complex, BRM remodeler, CHD assay, CHD atpase, CHD complex, CHD remodeler, INO80, INO80 assay, INO80 atpase, INO80 complex, INO80 remodeler, ISWI assay, ISWI atpase, ISWI complex, ISWI remodeler, Mi-2, Mi-2 assay, Mi-2 atpase, Mi-2 complex, Mi-2 remodeler, NuRD, NuRD assay, NuRD atpase, NuRD complex, NuRD remodeler, SMARCA assay, SMARCA atpase, SMARCA complex, SMARCA remodeler, SMARCA4 assay, SMARCA4 atpase, SMARCA4 complex, SMARCA4 remodeler, SNF2A, SNF2A assay, SNF2A atpase, SNF2A complex, SNF2A remodeler, SNF2B, SNF2B assay, SNF2B atpase, SNF2B complex, SNF2B remodeler, SNF2L2, SNF2L2 assay, SNF2L2 atpase, SNF2L2 complex, SNF2L2 remodeler, SNF2L4 assay, SNF2L4 atpase, SNF2L4 complex, SNF2L4 remodeler, SWI/SNF, SWI/SNF assay, SWI/SNF atpase, SWI/SNF complex, SWI/SNF remodeler, SWR1 assay, SWR1 atpase, SWR1 complex, SWR1 remodeler, chromatin remodeler, nucleosome remodeler, nucleosome remodeling assay, chromatin remodeling assay, nucleosome remodeling, chromatin remodeling complex, nucleosome remodeling complex, BRG1, nucleosome sliding, nucleosome sliding assay, nucleosome atpase, nucleosome mobilization","show_quantity_input":1,"title":"EpiDyne® Nucleosome Remodeling Assay Substrate 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Description
Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 217 base pair DNA sequence that includes the Widom 601 sequence with an added GATC.
Validation Data
Figure 1: DNA Gel Data
Free DNA
(DNA, 200 ng) and EpiDyne Nucleosome Remodeling Assay
Substrate ST601-GATC1 (Nuc, 370 ng) run on an acrylamide gel
and stained with ethidium bromide to visualize DNA.
Note absence of free DNA in "Nuc", indicating
all DNA complexed with histones.
Figure 2: Protein Gel Data
Coomassie stained PAGE gel of proteins in EpiDyne Nucleosome
Remodeling Assay Substrate ST601-GATC1 (2 µg) demonstrates
the purity of the histones in the preparation. Sizes of
molecular weight markers and positions of the core histones
(H2A, H2B, H3 and H4) are indicated.
Figure 3: Nucleosome Remodeling Data
RSC/ATP-dependent chromatin remodeling reaction in the
presence of DpnII restriction enzyme. EpiDyne Nucleosome
Remodeling Assay Substrate ST601-GATC1 was incubated with
RSC chromatin remodeling complex (10 nm) or without (No RSC)
for up to two hours in the presence of 2 mM ATP (or No ATP,
as indicated) and 50U of DpnII. Samples were quenched at
specified intervals and run on 8% native PAGE gel and
stained with ethidium bromide. The apperance of a band at
~200 base pairs (red arrow) indicates cleavage at GATC
subsequent to chromatin remodeling.
Technical Information
Purified recombinant mononucleosomes 1.70 mg/ml (DNA + protein weight) in 29.4 µl of 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 242,900 Da. Molarity = 7.00 µM.
Application Notes
This product is a substrate for chromatin remodeling assays (e.g. ACF Remodeling Enzyme Complex) using restriction enzymes to determine accessibility of GATC subsequent to chromatin remodeling.
DNA Sequence
CTAGGAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGGTCG TAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCG CGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGT CAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGA TGGATGATGATGGATGAATAGATGGATGGATGAAGCTTTAGG