EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated

$115.00

SKU: 18-4112
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1-2-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":658,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/658/947/epidyne-remodeling-assay-substrate-dna-st601-gatc12-biotinylated__78976.1645734539.jpg?c=2","alt":"EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=658","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"577","name":"Pack Size","value":"50 µg"}],"sku":"18-4112","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\">EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Description: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is a 217 base-pair double-stranded DNA fragment containing a 5' biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see 18-0005) as well as a 3' acceptor sequence to accomodate the histone octamer subsequent to remodeling. ST601-GATC1,2, Biotinylated DNA has a restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling. </td> </tr> <tr> <td valign=\"top\">EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Formulation: 50 µg lyophilyzed ST601-GATC1,2, Biotinylated DNA. </td> </tr> <tr> <td valign=\"top\"> EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Storage and Stability: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C. </td> </tr> <tr> <td valign=\"top\">EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Application Notes: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is useful as a control for restriction enzyme accessibility nucleosome remodeling assays using the EpiDyne Remodeling Assay Substrate, as a DpnII restriction enzyme site is present within the 601 sequence.\n\n \n \n DNA Sequence: GAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGATCGTAGACAGCTCTAGCACCGCTTAA \nACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCAC \nGTGTCAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGATGGATGATGATGGATG \nAATAGATGGATGGATGAAGCTT </td> </tr> <tr> <td valign=\"top\"> References: Lowary PT and J Widom (1998). J Mol Biol 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a> </td> </tr> <tr> <td valign=\"top\"> View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/18-4112.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"18-4112 Datasheet\" width=\"30\" height=\"40\" /></a> </td> </tr> </tbody> </table> </td> <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/18-4112-DNA-gel.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/18-4112-DNA-gel.jpg\" alt=\"18-4112 Gel Data\" width=\"241\" height=\"400\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"> DNA Gel Data: Lane 1: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated (200ng) and Lane2: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated (200 ng) digested with 10U DpnII incubated at 37C for 1 hour resolved via native PAGE. Migration positions of DNA molecular weight markers are indicated. (Click image to enlarge) </td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div>\n\n\n\n</body>\n</html>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$115.00","value":115,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":656,"sku":"16-4112","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-2-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":"\n\n\n \n\n \n\n \n\n\n\n EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated 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EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Description: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is a 217 base-pair double-stranded DNA fragment containing a 5' biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see 18-0005) as well as a 3' acceptor sequence to accomodate the histone octamer subsequent to remodeling. ST601-GATC1,2, Biotinylated DNA has a restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling.

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Formulation: 50 µg lyophilyzed ST601-GATC1,2, Biotinylated DNA.

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Storage and Stability: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated Application Notes: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is useful as a control for restriction enzyme accessibility nucleosome remodeling assays using the EpiDyne Remodeling Assay Substrate, as a DpnII restriction enzyme site is present within the 601 sequence.

DNA Sequence: GAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGATCGTAGACAGCTCTAGCACCGCTTAA
ACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCAC
GTGTCAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGATGGATGATGATGGATG
AATAGATGGATGGATGAAGCTT

References:
Lowary PT and J Widom (1998). J Mol Biol 276: 19-42. Link

View technical datasheet for this product.

DNA Gel Data: Lane 1: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated (200ng) and Lane2: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated (200 ng) digested with 10U DpnII incubated at 37C for 1 hour resolved via native PAGE. Migration positions of DNA molecular weight markers are indicated.
(Click image to enlarge)

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