EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated

$115.00

SKU: 18-4113
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1-2-3-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":659,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/659/667/epidyne_DNA__17108.1574793595.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=659","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"578","name":"Pack Size","value":"50 µg"}],"sku":"18-4113","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\">EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated Description: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated is a 217 base-pair double-stranded DNA fragment containing a 5' biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see 18-0005) as well as a 3' acceptor sequence to accomodate the histone octamer subsequent to remodeling. ST601-GATC1,2,3, Biotinylated DNA has a restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling. </td> </tr> <tr> <td valign=\"top\">EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated Formulation: 50 µg lyophilyzed ST601-GATC1,2,3, Biotinylated DNA. </td> </tr> <tr> <td valign=\"top\"> EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated Storage and Stability: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C. </td> </tr> <tr> <td valign=\"top\">EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated Application Notes: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated is useful as a control for restriction enzyme accessibility nucleosome remodeling assays using the EpiDyne Remodeling Assay Substrate, as a DpnII restriction enzyme site is present within the 601 sequence.\n\n \n \n DNA Sequence: GAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGATCGTAGACAGATCTAGCACCGCTTAA \nACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCAC \nGTGTCAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGATGGATGATGATGGATG \nAATAGATGGATGGATGAAGCTT </td> </tr> <tr> <td valign=\"top\"> References: Lowary PT and J Widom (1998). J Mol Biol 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a> </td> </tr> <tr> <td valign=\"top\"> View technical datasheet for this product. <a href=\"#\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"18-4113 Datasheet\" width=\"30\" height=\"40\" /></a> </td> </tr> </tbody> </table> </td> <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/18-4113-DNA-gel.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/18-4113-DNA-gel.jpg\" alt=\"18-4113 Gel Data\" width=\"241\" height=\"400\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"> DNA Gel Data: Lane 1: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated (200ng) and Lane2: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated (200 ng) digested with 10U DpnII incubated at 37C for 1 hour resolved via native PAGE. Migration positions of DNA molecular weight markers are indicated. (Click image to enlarge) </td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div>\n\n\n\n</body>\n</html>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$115.00","value":115,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":657,"sku":"16-4113","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-2-3-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":"\n\n\n \n\n \n\n \n\n\n\n EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3 Biotinylated Description: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/657/809/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__52268.1620836268.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/657/809/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__52268.1620836268.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, Biotinylated"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/657/808/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_no_background_RGB_AL_MP__61044.1620836268.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, Biotinylated"}],"date_added":"25th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":575,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=657","price":{"without_tax":{"currency":"USD","formatted":"$565.00","value":565},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=657"},{"id":869,"sku":"18-4111","name":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Nucleosomes/Nucleosome Components and Subunits"],"summary":"\n \n \n \n Description\n \n \n EpiD","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/869/970/epidyne-remodeling-assay-substrate-dna-st601-gatc1-biotinylated__84056.1645734562.jpg?c=2","alt":"EpiDyne Remodeling Assay Substrate DNA ST601-GATC1, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/869/970/epidyne-remodeling-assay-substrate-dna-st601-gatc1-biotinylated__84056.1645734562.jpg?c=2","alt":"EpiDyne Remodeling Assay Substrate DNA ST601-GATC1, Biotinylated"}],"date_added":"12th Jul 2021","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":882,"name":"Pack Size","value":"50 µg"},{"id":883,"name":"Internal Comment","value":"waiting on full webpage to be made by Website Team."}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=869","price":{"without_tax":{"currency":"USD","formatted":"$115.00","value":115},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=869"},{"id":873,"sku":"18-4110","name":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc0-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Nucleosomes/Nucleosome Components and Subunits"],"summary":"\n \n \n \n Description\n \n \n EpiD","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/873/900/DNA__13746.1569012501.190.250__90864.1626713525.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/873/900/DNA__13746.1569012501.190.250__90864.1626713525.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0, Biotinylated"}],"date_added":"19th Jul 2021","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":894,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.00 LBS","value":0},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=873","price":{"without_tax":{"currency":"USD","formatted":"$115.00","value":115},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=873"},{"id":658,"sku":"18-4112","name":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1-2-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":" EpiDyne Remode","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/658/947/epidyne-remodeling-assay-substrate-dna-st601-gatc12-biotinylated__78976.1645734539.jpg?c=2","alt":"EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/658/947/epidyne-remodeling-assay-substrate-dna-st601-gatc12-biotinylated__78976.1645734539.jpg?c=2","alt":"EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated"}],"date_added":"25th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":577,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=658","price":{"without_tax":{"currency":"USD","formatted":"$115.00","value":115},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=658"},{"id":726,"sku":"16-4116","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, 50-N-66, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-2-3-50-n-66-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":" \n\n \n\n \n\n\n\n EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, 50-N-66, Biotinylated Description: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/726/814/2021.02.25_EPI_Nucleosome_thumbnail_only_50N66_white_background_RBG_AL_MP__54028.1620836268.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, 50-N-66, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/726/814/2021.02.25_EPI_Nucleosome_thumbnail_only_50N66_white_background_RBG_AL_MP__54028.1620836268.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, 50-N-66, Biotinylated"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/726/928/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc123-50-n-66-biotinylated__93355.1645734519.jpg?c=2","alt":"EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2,3, 50-N-66, Biotinylated"}],"date_added":"9th Jan 2020","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":943,"name":"Pack Size","value":"50 μg"},{"id":944,"name":"Internal Comment","value":"Bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=726","price":{"without_tax":{"currency":"USD","formatted":"$565.00","value":565},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=726"},{"id":373,"sku":"18-4100","name":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc0","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Nucleosomes/Nucleosome Components and Subunits"],"summary":"\n \n \n \n \n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/373/291/DNA__13746.1569012501.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/373/291/DNA__13746.1569012501.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0"}],"date_added":"5th Jul 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":602,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=373","price":{"without_tax":{"currency":"USD","formatted":"$115.00","value":115},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=373"},{"id":374,"sku":"18-4101","name":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Nucleosomes/Nucleosome Components and Subunits"],"summary":"\n \n \n \n Description\n \n \n EpiDyne® Remodeling Assay Sub","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/374/292/DNA__93069.1614637188.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/374/292/DNA__93069.1614637188.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1"}],"date_added":"5th Jul 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":637,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=374","price":{"without_tax":{"currency":"USD","formatted":"$115.00","value":115},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=374"},{"id":470,"sku":"16-4111","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":" \n\n \n\n \n\n\n\n EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated Description: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/470/805/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__98781.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/470/805/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_white_background_RGB_AL_MP__98781.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/470/804/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_no_background_RGB_AL_MP__77219.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated"}],"date_added":"4th Oct 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1055,"name":"Pack Size","value":"50 μg"},{"id":1056,"name":"Internal Comment","value":"Bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=470","price":{"without_tax":{"currency":"USD","formatted":"$565.00","value":565},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=470"},{"id":656,"sku":"16-4112","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-2-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":"\n\n\n \n\n \n\n \n\n\n\n EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Description: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/656/971/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc12-biotinylated__56258.1645734563.jpg?c=2","alt":"EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/656/971/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc12-biotinylated__56258.1645734563.jpg?c=2","alt":"EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/656/807/2021.02.25_EPI_Nucleosome_thumbnail_only_6N66_no_background_RGB_AL_MP__54519.1620836275.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated"}],"date_added":"25th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":576,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=656","price":{"without_tax":{"currency":"USD","formatted":"$565.00","value":565},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=656"}],"shipping_messages":[],"rating":0,"meta_keywords":"","show_quantity_input":1,"title":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/659/667/epidyne_DNA__17108.1574793595.gif?c=2","alt":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated"}]} Pack Size: 50 µg

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated Description: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated is a 217 base-pair double-stranded DNA fragment containing a 5' biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see 18-0005) as well as a 3' acceptor sequence to accomodate the histone octamer subsequent to remodeling. ST601-GATC1,2,3, Biotinylated DNA has a restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling.

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated Formulation: 50 µg lyophilyzed ST601-GATC1,2,3, Biotinylated DNA.

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated Storage and Stability: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated Application Notes: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated is useful as a control for restriction enzyme accessibility nucleosome remodeling assays using the EpiDyne Remodeling Assay Substrate, as a DpnII restriction enzyme site is present within the 601 sequence.

DNA Sequence: GAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGATCGTAGACAGATCTAGCACCGCTTAA
ACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCAC
GTGTCAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGATGGATGATGATGGATG
AATAGATGGATGGATGAAGCTT

References:
Lowary PT and J Widom (1998). J Mol Biol 276: 19-42. Link

View technical datasheet for this product.

DNA Gel Data: Lane 1: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated (200ng) and Lane2: EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2,3, Biotinylated (200 ng) digested with 10U DpnII incubated at 37C for 1 hour resolved via native PAGE. Migration positions of DNA molecular weight markers are indicated.
(Click image to enlarge)

Current stock: 0