SKU: 16-0019
{"url":"https://www.epicypher.com/products/nucleosomes/chicken-mononucleosomes-purified","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":467,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Purified chicken nucleosomes suitable for enzyme screening and kinetic assays","category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/467/340/Nuc-3B__00718.1516390305.png?c=2","alt":"Chicken Mononucleosomes, Purified"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=467","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"235","name":"Pack Size","value":"50 μg"}],"sku":"16-0019","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"><tbody><tr valign=\"top\"><td><table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Chicken Mononucleosomes, Purified Description:</b> Mononucleosomes purified from chicken erythrocytes. The nucleosome is the basic subunit of chromatin consisting of the histone octamer (two each of the four core histones, H2A, H2B, H3 and H4) wrapped by 147 base pairs of DNA. <br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Chicken Mononucleosomes, Purified Formulation: </b>Purified Chicken Mononucleosomes in 10 mM Tris-HCl pH 8.0, 1 mM EDTA and 10% glycerol. Approx. MW = 225,000 Da Approx. concentration = 2.3 µM.<b><br /><br /></b></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Chicken Mononucleosomes, Purified Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Chicken Mononucleosomes, Purified Application Notes:</b> Chicken Mononucleosomes are suitable for use in enzyme assays such as acetylation, methylation or phosphorylation, chromatin binding assays, or for use as a positive control in Western blotting.</strong><br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><b style=\"font-size: 12pt; \">References:</b><br /><span style=\"font-size: 10pt; \">Adhvaryu KK et al (2005). <em>Eukaryot Cell</em> 4: 1455-1564. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/16087750\" target=\"_new\">Link</a><br />Kizer KO et al (2005). <em>Mol Cell Biol</em> 25: 3305-3316. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/15798214\" target=\"_new\">Link</a><br />Morris SA et al (2005). <em>Eukaryot Cel</em>l 4: 1446-1454. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/16087749\" target=\"_new\">Link</a></span><br /><br /></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0019.pdf\" target=\"_blank\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0019 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p></td></tr></tbody></table></td><td><table width=\"300\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16_0019_dna_gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16_0019_dna_gel.jpg\" alt=\"16-0019 DNA Gel\" width=\"133\" /></a></td></tr><tr>\n <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> DNA was purified from Chicken Oligoucleosomes (1 µg) and run on an agarose gel to show the size of nucleosomal DNA compared to molecular weight markers (base pairs). Note that mononucleosomal DNA runs at roughly 150 base pairs.<br />\n <strong>(Click image to enlarge) </strong></span></span></td></tr><tr valign=\"top\"><td valign=\"top\" align=\"center\"><a href=\"/content/images/products/nucleosomes/16_0019_protein_gel.jpg\" target=\"_new\"> <img src=\"/content/images/products/nucleosomes/16_0019_protein_gel.jpg\" alt=\"16-0019 Protein Gel\" width=\"150\" /></a></td></tr><tr>\n <td valign=\"top\" align=\"left\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Chicken Mononucleosomes (2 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.<br />\n <strong>(Click image to enlarge) </strong></span></span></td></tr><tr valign=\"top\"><td valign=\"top\" align=\"center\"><a href=\"/content/images/products/nucleosomes/16_0019_enzyme_gel.jpg\" target=\"_new\"> <img src=\"/content/images/products/nucleosomes/16_0019_enzyme_gel.jpg\" alt=\"16-0019 Protein Gel\" width=\"152\" height=\"125\" /></a></td></tr><tr>\n <td valign=\"top\" align=\"left\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Histone Methyltransferase Data:</strong> Chicken Mononucleosomes were used in a radioactive histone methyltransferase assay with recombinant G9a. Radioactive SAM was incubated with Chicken Mononucleosmes in the absence (-) or presence (+) of recombinant G9a and run on an acrylamide gel. HMTase activity co-purifying with the nucleosomes is undetectable. <strong>Left Top:</strong> Coomassie stained gel. <strong>Left Bottom:</strong> autoradiogram of gel. <strong>Right:</strong> Densitometry quantification of autoradiogram.<br />\n <strong>(Click image to enlarge) </strong></span></span></td></tr></tbody></table></td></tr></tbody></table>\n\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$215.00","value":215,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Chicken Mononucleosomes, Purified | suitable for enzyme screening and kinetic assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":94,"sku":"16-0002","name":"HeLa Mononucleosomes, Purified","url":"https://www.epicypher.com/products/nucleosomes/hela-mononucleosomes-purified","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"summary":"\n \n \n Species: Human\n \n \n Source: HeLa cells\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/94/334/Human_recombinant_nucleosome__78345.1516390310.gif?c=2","alt":"HeLa Mononucleosomes, Purified"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/94/334/Human_recombinant_nucleosome__78345.1516390310.gif?c=2","alt":"HeLa Mononucleosomes, Purified"}],"date_added":"3rd Dec 2014","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":11,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=94","price":{"without_tax":{"currency":"USD","formatted":"$495.00","value":495},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=94"},{"id":101,"sku":"16-0004","name":"Chicken Polynucleosomes, Purified","url":"https://www.epicypher.com/products/nucleosomes/chicken-polynucleosomes-purified","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"summary":"Chicken Oligonucleosomes, Purified Description: Oligonucleosomes purified from chicken erythroc","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/101/341/Nuc-3B__80947.1516390305.png?c=2","alt":"Chicken Polynucleosomes, Purified"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/101/341/Nuc-3B__80947.1516390305.png?c=2","alt":"Chicken Polynucleosomes, Purified"}],"date_added":"3rd Dec 2014","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1035,"name":"Pack Size","value":"50 μg"},{"id":1036,"name":"Internal Comment","value":"Has Bulk in LG"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=101","price":{"without_tax":{"currency":"USD","formatted":"$215.00","value":215},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=101"}],"shipping_messages":[],"rating":0,"meta_keywords":"chicken nucleosome, chicken nucleosome, mononucleosome, avian mononucleosomes, chromatin, octamer, nucleosomes, enzyme assays, acetylation, enzyme screening, enzyme kinetics, methylation, chromatin binding assays, Western blotting, chromatin, epigenetics, 16-0019, chromatin","show_quantity_input":1,"title":"Chicken Mononucleosomes, Purified","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/467/340/Nuc-3B__00718.1516390305.png?c=2","alt":"Chicken Mononucleosomes, Purified"}]}
Pack Size: 50 μg
