CUTANA™ CUT&RUN Library Prep Kit
{"url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":889,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"The all-inclusive Library Prep solution specifically optimized for CUT&RUN","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/889/997/Epicypher_CUTANA_CUTRUN_LibraryPrep_Box__64431.1646406521.png?c=2","alt":"CUTANA™ CUT&RUN Library Prep Kit"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=889","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"983","name":"Pack Size","value":"48 Reactions"}],"sku":"14-1001","description":"<div class=\"product-general-info\">\n <ul style=\"display: none\" class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"display: none\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div\n id=\"w-s-3835-14-1001\"\n style=\"\n width: max-content;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-14-1001-div\">\n <a\n id=\"bioz-w-pb-14-1001\"\n style=\"font-size: 12px; color: transparent\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n </a></div\n ></a>\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n The CUTANA™ CUT&RUN Library Prep Kit offers high fidelity library\n generation for Illumina<sup>®</sup> sequencing by harnessing the\n power of New England Biolabs<sup>®</sup> best-in-class NEBNext<sup\n >®</sup\n >\n reagents. The kit offers a streamlined protocol specifically\n optimized for high sensitivity CUT&RUN applications, including those\n with low cell inputs.\n </p>\n <p\n style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a\n target=\"_blank\"\n style=\"color: #fff\"\n href=\"/contact-us/request-a-quote/\"\n >Request a quote to save 10% when you buy our Library Prep and\n CUT&RUN Kits together (USA only)\n </a>\n </p>\n <p>\n Included are all necessary reagents to perform end repair, adaptor\n ligation, combinatorial dual indexing for multiplexing up to 48\n samples, and DNA cleanup with SPRIselect reagent from Beckman\n Coulter, Inc. (see Additional Information) If additional\n multiplexing is desired, Primer Sets 1 and 2 can be combined for\n sequencing of up to 96 samples in a single lane. Pairing this kit\n with the EpiCypher CUT&RUN Kit (EpiCypher\n <a\n target=\"_blank\"\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >) affords users a cells-to-sequencing solution for chromatin\n mapping experiments with all the necessary controls and validated\n reagents to ensure confidence in obtaining high quality data. To\n place bulk orders, contact\n <a href=\"mailto:info@epicypher.com\">info@epicypher.com</a>.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14_1001_input_dilution.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"14_1001_input_dilution\"\n src=\"/content/images/products/kits/14_1001_input_dilution.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: High quality sequencing libraries from as\n little as 0.5 ng of input</strong\n ><br />\n Gene browser shots generated using the Integrative Genomics\n Viewer (IGV, Broad Institute) show a representative 137 kb\n window for H3K27me3 antibody (ABclonal A16199) tested in\n CUT&RUN using 500k K562 cells. Decreasing inputs of CUT&RUN\n enriched DNA were used in the library prep protocol to\n simulate low abundance targets or low cell input\n experiments. Data are largely indistinguishable across\n inputs, demonstrating robust preparation of Illumina<sup\n >®</sup\n >\n NGS-ready libraries.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14_1001_distribution_analysis.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"14_1001_distribution_analysis\"\n src=\"/content/images/products/kits/14_1001_distribution_analysis.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: CUT&RUN DNA Fragment Size Distribution\n Analysis</strong\n ><br />\n CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit\n (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >) starting with 500k K562 cells. Five nanograms of CUT&RUN\n output DNA from reactions utilizing IgG (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >), H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >), H3K27me3 (ABclonal A16199), and CTCF (EpiCypher\n <a\n href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ctcf-cutana-cut-and-run-antibody\"\n >13-2014</a\n >) antibodies were prepared for paired-end Illumina<sup\n >®</sup\n >\n sequencing with the CUTANA CUT&RUN Library Prep Kit. Library\n DNA was analyzed by Agilent TapeStation<sup>®</sup>, which\n confirmed that mononucleosomes were predominantly enriched\n in CUT&RUN (~300 bp peaks represent 150 bp nucleosomes +\n sequencing adapters). Peaks at ~380 bp correspond to the\n SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/kits/14_1001_target_compatibility.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n loading=\"lazy\"\n alt=\"14_1001_target_compatibility\"\n src=\"/content/images/products/kits/14_1001_target_compatibility.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Representative gene browser tracks</strong\n ><br />\n\n CUT&RUN sequencing libraries described in Figure 2 were\n sequenced on an Illumina<sup>®</sup> NextSeq 2000 with a P3\n cartridge (paired-end 2x50 cycle). Data were aligned to the\n hg19 genome using Bowtie2. Data were filtered to remove\n duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions. A\n representative 640 kb window at the SEPTIN5 gene is shown\n for three replicates (\"Rep\") of IgG and H3K4me3 antibodies,\n as well as individual tracks for H3K27me3 and the\n transcription factor CTCF, demonstrating the robustness and\n reproducibility of the workflow with a variety of targets.\n Sequencing libraries prepared with the CUTANA CUT&RUN\n Library Prep kit produced the expected genomic distribution\n for each target. Sample sequencing depth was as follows\n (millions of reads): IgG Rep 1 (20.1), IgG Rep 2 (16.6), IgG\n Rep 3 (16.1), H3K4me3 Rep 1 (7.3), H3K4me3 Rep 2 (17.2),\n H3K4me3 Rep 3 (13.8), H3K27me3 (13.4), CTCF (16.2). Images\n were generated using the Integrative Genomics Viewer (IGV,\n Broad Institute).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"14_1001_input_dilution\"\n src=\"/content/images/products/kits/14_1001_input_dilution.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"14_1001_distribution_analysis\"\n src=\"/content/images/products/kits/14_1001_distribution_analysis.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"14_1001_target_compatibility\"\n src=\"/content/images/products/kits/14_1001_target_compatibility.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Kit Contents</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes\"\n >10-0009</a\n >p\n </td>\n </tr>\n\n <tr>\n <td>SPRIselect reagent manufactured by Beckman Coulter, Inc.</td>\n <td>21-1405p</td>\n </tr>\n\n <tr>\n <td>0.1X TE Buffer</td>\n <td>21-1025p</td>\n </tr>\n\n <tr>\n <td>End Prep Enzyme</td>\n <td>15-1019p</td>\n </tr>\n\n <tr>\n <td>End Prep Buffer</td>\n <td>21-1012p</td>\n </tr>\n\n <tr>\n <td>Adapter for Illumina®</td>\n <td>18-1000p</td>\n </tr>\n\n <tr>\n <td>Ligation Mix</td>\n <td>15-1020p</td>\n </tr>\n\n <tr>\n <td>Ligation Enhancer</td>\n <td>15-1021p</td>\n </tr>\n\n <tr>\n <td>U-Excision Enzyme</td>\n <td>15-1023p</td>\n </tr>\n\n <tr>\n <td>CUTANA™ Hot-Start 2X PCR Master Mix</td>\n <td>15-1022p</td>\n </tr>\n\n <tr>\n <td style=\"vertical-align: top\">Multiplexing Primers</td>\n <td>\n Primer Set 1 includes i701-i706<br />\n Primer Set 2 includes i707-i712<br />\n Both Sets include i501-i508\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Accessory Products</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA™ ChIC/CUT&RUN Kit</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >\n </td>\n </tr>\n\n <tr>\n <td>CUT&RUN Antibodies</td>\n <td>\n <a href=\"/products/antibodies/cutana-cut-and-run-antibodies\"\n >See the list</a\n >\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">Magnetic Separation Rack, 0.2 mL Tubes</td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-run-assays/magnetic-separation-rack-0-2-ml-tubes\"\n >10-0008</a\n >\n </td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n OPEN KIT IMMEDIATELY and store components at room temperature\n and -20°C as indicated (see Kit Manual for full instructions).\n Stable for 6 months upon date of receipt.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for Use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n See Kit Manual\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n New England Biolabs<sup>®</sup>, NEB<sup>®</sup>, and NEBNext<sup\n >®</sup\n >\n are registered trademarks of New England Biolabs, Inc.\n </p>\n <p>\n This Product is covered by one or more patents, trademarks and/or copyrights \n owned or controlled by NEB. While NEB develops and validates its products for \n various applications, the use of this product may require the purchaser to \n obtain additional third-party intellectual property rights for certain \n applications.\n </p>\n <p>\n This product is licensed for research and commercial use from Bio-Rad \n Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, \n 7,666,645, and corresponding patents in other countries. No rights are granted \n for use of the product for Digital PCR or real-time PCR applications, with the \n exception of quantification in Next Generation Sequencing workflows.\n </p>\n <p>\n Beckman Coulter, the stylized logo, and SPRIselect are trademarks or\n registered trademarks of Beckman Coulter, Inc. in the United States\n and other countries.\n </p>\n </div>\n </div>\n </div>\n\n <h3\n style=\"color: #4698cb; font-size: 24px; border-bottom: none\"\n class=\"custom-title-description\">\n Documents & Resources\n </h3>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <!-- <h3 style=\"color: #4698cb; margin-bottom: 0\">Current Lot</h3> -->\n <h3 class=\"sub-title1\">Kit Version 1</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/14-1001.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Technical Datasheet (14-1001)</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/14-1002.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n 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M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >CUT&RUN Library Prep Kit Manual</span\n >\n </a>\n </div>\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/manuals/14-1001-2-cut-and-run-library-prep-kit-quick-card.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n 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Description
The CUTANA™ CUT&RUN Library Prep Kit offers high fidelity library generation for Illumina® sequencing by harnessing the power of New England Biolabs® best-in-class NEBNext® reagents. The kit offers a streamlined protocol specifically optimized for high sensitivity CUT&RUN applications, including those with low cell inputs.
Request a quote to save 10% when you buy our Library Prep and CUT&RUN Kits together (USA only)
Included are all necessary reagents to perform end repair, adaptor ligation, combinatorial dual indexing for multiplexing up to 48 samples, and DNA cleanup with SPRIselect reagent from Beckman Coulter, Inc. (see Additional Information) If additional multiplexing is desired, Primer Sets 1 and 2 can be combined for sequencing of up to 96 samples in a single lane. Pairing this kit with the EpiCypher CUT&RUN Kit (EpiCypher 14-1048) affords users a cells-to-sequencing solution for chromatin mapping experiments with all the necessary controls and validated reagents to ensure confidence in obtaining high quality data. To place bulk orders, contact info@epicypher.com.
Validation Data
Figure 1: High quality sequencing libraries from as
little as 0.5 ng of input
Gene browser shots generated using the Integrative Genomics
Viewer (IGV, Broad Institute) show a representative 137 kb
window for H3K27me3 antibody (ABclonal A16199) tested in
CUT&RUN using 500k K562 cells. Decreasing inputs of CUT&RUN
enriched DNA were used in the library prep protocol to
simulate low abundance targets or low cell input
experiments. Data are largely indistinguishable across
inputs, demonstrating robust preparation of Illumina®
NGS-ready libraries.
Figure 2: CUT&RUN DNA Fragment Size Distribution
Analysis
CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit
(EpiCypher
14-1048) starting with 500k K562 cells. Five nanograms of CUT&RUN
output DNA from reactions utilizing IgG (EpiCypher
13-0042), H3K4me3 (EpiCypher
13-0041), H3K27me3 (ABclonal A16199), and CTCF (EpiCypher
13-2014) antibodies were prepared for paired-end Illumina®
sequencing with the CUTANA CUT&RUN Library Prep Kit. Library
DNA was analyzed by Agilent TapeStation®, which
confirmed that mononucleosomes were predominantly enriched
in CUT&RUN (~300 bp peaks represent 150 bp nucleosomes +
sequencing adapters). Peaks at ~380 bp correspond to the
SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher
19-1002).
Figure 3: Representative gene browser tracks
CUT&RUN sequencing libraries described in Figure 2 were
sequenced on an Illumina® NextSeq 2000 with a P3
cartridge (paired-end 2x50 cycle). Data were aligned to the
hg19 genome using Bowtie2. Data were filtered to remove
duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions. A
representative 640 kb window at the SEPTIN5 gene is shown
for three replicates ("Rep") of IgG and H3K4me3 antibodies,
as well as individual tracks for H3K27me3 and the
transcription factor CTCF, demonstrating the robustness and
reproducibility of the workflow with a variety of targets.
Sequencing libraries prepared with the CUTANA CUT&RUN
Library Prep kit produced the expected genomic distribution
for each target. Sample sequencing depth was as follows
(millions of reads): IgG Rep 1 (20.1), IgG Rep 2 (16.6), IgG
Rep 3 (16.1), H3K4me3 Rep 1 (7.3), H3K4me3 Rep 2 (17.2),
H3K4me3 Rep 3 (13.8), H3K27me3 (13.4), CTCF (16.2). Images
were generated using the Integrative Genomics Viewer (IGV,
Broad Institute).
Kit Contents
Item | Cat. No. |
---|---|
CUTANA™ CUT&RUN 8-strip 0.2 mL Tubes | 10-0009p |
SPRIselect reagent manufactured by Beckman Coulter, Inc. | 21-1405p |
0.1X TE Buffer | 21-1025p |
End Prep Enzyme | 15-1019p |
End Prep Buffer | 21-1012p |
Adapter for Illumina® | 18-1000p |
Ligation Mix | 15-1020p |
Ligation Enhancer | 15-1021p |
U-Excision Enzyme | 15-1023p |
CUTANA™ Hot-Start 2X PCR Master Mix | 15-1022p |
Multiplexing Primers |
Primer Set 1 includes i701-i706 Primer Set 2 includes i707-i712 Both Sets include i501-i508 |
Recommended Accessory Products
Item | Cat. No. |
---|---|
CUTANA™ ChIC/CUT&RUN Kit | 14-1048 |
CUT&RUN Antibodies | See the list |
Magnetic Separation Rack, 0.2 mL Tubes | 10-0008 |
Technical Information
Additional Information
New England Biolabs®, NEB®, and NEBNext® are registered trademarks of New England Biolabs, Inc.
This Product is covered by one or more patents, trademarks and/or copyrights owned or controlled by NEB. While NEB develops and validates its products for various applications, the use of this product may require the purchaser to obtain additional third-party intellectual property rights for certain applications.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
Beckman Coulter, the stylized logo, and SPRIselect are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.