Menin CUTANA™ CUT&RUN Antibody
{"url":"https://www.epicypher.com/products/antibodies/cutana-cut-run-antibodies/cut-run-antibodies-chromatin-associated-proteins/menin-cutana-cut-run-antibody","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":906,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Rabbit polyclonal Menin antibody rigorously tested for robust and reliable performance in CUT&RUN assays. Also validated for IHC, IP, & WB.","category":["Antibodies/CUTANA™ CUT&RUN Antibodies","Antibodies/CUTANA™ CUT&RUN Antibodies/CUT&RUN Antibodies - Chromatin-Associated Proteins","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/906/1010/chd3-cutana-cutandrun-antibody__66975.1645734525__91145.1648666822__40012.1648670522.jpg?c=2","alt":"Menin CUTANA™ CUT&RUN Antibody"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=906","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1030","name":"Pack Size","value":"100 µL"}],"sku":"13-2021","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type: </strong>Polyclonal\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Host: </strong>Rabbit\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Applications: </strong>CUT&RUN, IHC, IP, WB\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Reactivity: </strong>Human, Mouse\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Format: </strong>Antigen affinity-purified\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Target Size: </strong>68 kDa\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n This antibody meets EpiCypher’s \"CUTANA Compatible\" criteria for\n performance in Cleavage Under Targets and Release Using Nuclease\n (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag)\n approaches to genomic mapping. Every lot of a CUTANA Compatible\n antibody is tested in the indicated approach using EpiCypher\n optimized <a href=\"/resources/protocols/\">protocols</a> and\n determined to yield peaks that show a genomic distribution pattern\n consistent with reported function(s) of the target protein. Menin\n antibody produces CUT&RUN peaks above background primarily in\n promoter and intronic regions (<strong>Figure 1</strong>) that\n overlap with H3K4me3 (<strong>Figure 2</strong>), consistent with\n its known role as a component of MLL/SET1 histone methyltransferase\n complex, where it specifically methylates H3K4. As a part of the\n MLL/SET1 complex, Menin mediates its tumor suppressor activity by\n regulating histone methylation of HOX and CDK inhibitor genes [1].\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n CUT&RUN was performed on 500k K562 cells with 0.5 µg of either Menin\n or IgG negative control (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >). Library preparation was performed with 5 ng of DNA (or the total\n amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN\n Library Prep Kit (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n >14-1001/14-1002</a\n >). Both kit protocols were adapted for high throughput Tecan liquid\n handling. Libraries were run on an Illumina NextSeq2000 with\n paired-end sequencing (2x50 bp). Sample sequencing depth was 5.0\n million reads (IgG), 8.7 million reads (Menin), and 7.0 million\n reads (H3K4me3). Data were aligned to the hg19 genome using Bowtie2.\n Data were filtered to remove duplicates, multi-aligned reads, and\n ENCODE DAC Exclusion List regions.\n </p>\n\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2021-menin-peaks.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2021-menin-peaks\"\n src=\"/content/images/products/antibodies/13-2021-menin-peaks.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Menin peaks in CUT&RUN </strong><br />\n CUT&RUN was performed as described above. Peaks were called\n with MACS2. Heatmaps show Menin peaks relative to IgG\n negative control antibody in aligned rows ranked by\n intensity (top to bottom) and colored such that red\n indicates high localized enrichment and blue denotes\n background signal (<strong>left</strong>). The number of\n peaks that fall into distinct classes of functionally\n annotated genomic regions are shown\n (<strong>right</strong>).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2021-browser-tracks.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2021-browser-tracks\"\n src=\"/content/images/products/antibodies/13-2021-browser-tracks.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: Menin CUT&RUN representative browser tracks </strong\n ><br />\n CUT&RUN was performed as described above. Gene browser shots\n were generated using the Integrative Genomics Viewer (IGV,\n Broad Institute). Three representative loci show overlap of\n Menin and H3K4me3 peaks.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2021-immunohistochemistry-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2021-immunohistochemistry-data\"\n src=\"/content/images/products/antibodies/13-2021-immunohistochemistry-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 3: Immunohistochemistry data </strong><br />\n FFPE sections of human lung carcinoma\n (<strong>left</strong>) and human prostate carcinoma\n (<strong>right</strong>) using anti-Menin antibody at a\n dilution of 1:1,000.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2021-immunoprecipitation-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2021-immunoprecipitation-data\"\n src=\"/content/images/products/antibodies/13-2021-immunoprecipitation-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 4: Immunoprecipitation data </strong><br />\n EpiCypher Menin antibody (6 µg) was used to\n immunoprecipitate whole cell lysates (1 mg, 20% of IP\n loaded) isolated from HeLa cells. A negative control IgG\n antibody and positive control antibodies targeting Menin\n (Bethyl Laboratories) were also used to demonstrate\n specificity of the IP. EpiCypher 13-2021 and Bethyl\n A300-105A-11 target the same epitope, while Bethyl A300-115A\n targets a different epitope (between amino acids 500 and\n 550). For blotting immunoprecipitates, EpiCypher Menin\n antibody was used at a dilution of 1:25,000.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/antibodies/13-2021-western-blot-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img loading=\"lazy\"\n alt=\"13-2021-western-blot-data\"\n src=\"/content/images/products/antibodies/13-2021-western-blot-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 5: Western blot data </strong><br />\n Western analysis of Menin in whole cell extracts from HeLa,\n HEK293T, Jurkat, K562, and MCF-7 cells. Fifty micrograms of\n lysate was resolved via SDS-PAGE and detected with a\n 1:25,000 dilution of Menin antibody.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\"\n alt=\"13-2021-menin-peaks\"\n src=\"/content/images/products/antibodies/13-2021-menin-peaks.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2021-browser-tracks\"\n src=\"/content/images/products/antibodies/13-2021-browser-tracks.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2021-immunohistochemistry-data\"\n src=\"/content/images/products/antibodies/13-2021-immunohistochemistry-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2021-immunoprecipitation-data\"\n src=\"/content/images/products/antibodies/13-2021-immunoprecipitation-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img loading=\"lazy\"\n alt=\"13-2021-western-blot-data\"\n src=\"/content/images/products/antibodies/13-2021-western-blot-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunogen</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Between amino acids 575 and 615\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 1 year at 4°C from date of receipt\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Antigen affinity-purified antibody in Tris-citrate/phosphate\n buffer pH 7-8, 0.09% sodium azide\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Dilution</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>CUT&RUN:</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 0.5 µg per reaction\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunohistochemistry (IHC):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is\n recommended for FFPE tissue sections\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Immunoprecipitation (IP):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 2 - 10 µg/mg lysate\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Western Blot (WB):</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 1:10,000 - 1:25,000\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">O00255</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Gene Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">menin1, MEN1</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Protein Name</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">Menin</div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Alternate Names</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n MEA1, multiple endocrine adenomatosis 1, SCG2, suppressor\n candidate gene 2\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Target Size</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">68 kDa</div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Balogh et al. <em>Trends Endocrinol Metab</em> (2006). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/16997566/\"\n title=\"Menin and its interacting proteins: elucidation of menin function\"\n target=\"new\">\n 16997566</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/13-2021.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Additional Info</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n This antibody is provided for commercial sale under license from\n Bethyl Laboratories, Inc.\n </p>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$495.00","value":495,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Menin CUTANA™ CUT&RUN Antibody","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":772,"sku":"14-1048","name":"CUTANA™ ChIC/CUT&RUN Kit","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n \n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/772/998/Epicypher_CUTANA_CUTRUN_Box__31908.1646406725.png?c=2","alt":"CUTANA™ ChIC/CUT&RUN Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/772/998/Epicypher_CUTANA_CUTRUN_Box__31908.1646406725.png?c=2","alt":"CUTANA™ ChIC/CUT&RUN Kit"}],"date_added":"13th Aug 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- Type: Polyclonal
- Host: Rabbit
- Applications: CUT&RUN, IHC, IP, WB
- Reactivity: Human, Mouse
- Format: Antigen affinity-purified
- Target Size: 68 kDa
Description
This antibody meets EpiCypher’s "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. Menin antibody produces CUT&RUN peaks above background primarily in promoter and intronic regions (Figure 1) that overlap with H3K4me3 (Figure 2), consistent with its known role as a component of MLL/SET1 histone methyltransferase complex, where it specifically methylates H3K4. As a part of the MLL/SET1 complex, Menin mediates its tumor suppressor activity by regulating histone methylation of HOX and CDK inhibitor genes [1].
Validation Data
CUT&RUN was performed on 500k K562 cells with 0.5 µg of either Menin or IgG negative control (EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v2.0 (EpiCypher 14-1048). Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001/14-1002). Both kit protocols were adapted for high throughput Tecan liquid handling. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample sequencing depth was 5.0 million reads (IgG), 8.7 million reads (Menin), and 7.0 million reads (H3K4me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.
Figure 1: Menin peaks in CUT&RUN
CUT&RUN was performed as described above. Peaks were called
with MACS2. Heatmaps show Menin peaks relative to IgG
negative control antibody in aligned rows ranked by
intensity (top to bottom) and colored such that red
indicates high localized enrichment and blue denotes
background signal (left). The number of
peaks that fall into distinct classes of functionally
annotated genomic regions are shown
(right).
Figure 2: Menin CUT&RUN representative browser tracks
CUT&RUN was performed as described above. Gene browser shots
were generated using the Integrative Genomics Viewer (IGV,
Broad Institute). Three representative loci show overlap of
Menin and H3K4me3 peaks.
Figure 3: Immunohistochemistry data
FFPE sections of human lung carcinoma
(left) and human prostate carcinoma
(right) using anti-Menin antibody at a
dilution of 1:1,000.
Figure 4: Immunoprecipitation data
EpiCypher Menin antibody (6 µg) was used to
immunoprecipitate whole cell lysates (1 mg, 20% of IP
loaded) isolated from HeLa cells. A negative control IgG
antibody and positive control antibodies targeting Menin
(Bethyl Laboratories) were also used to demonstrate
specificity of the IP. EpiCypher 13-2021 and Bethyl
A300-105A-11 target the same epitope, while Bethyl A300-115A
targets a different epitope (between amino acids 500 and
550). For blotting immunoprecipitates, EpiCypher Menin
antibody was used at a dilution of 1:25,000.
Technical Information
Recommended Dilution
Gene & Protein Information
References
Documents & Resources
Additional Info
This antibody is provided for commercial sale under license from Bethyl Laboratories, Inc.