CUTANA™ ATAC-seq
ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) maps open chromatin genome-wide. The Tn5 transposase cuts accessible DNA and inserts sequencing adapters in a single tagmentation step, while closed, nucleosome-wrapped chromatin stays untagmented. The resulting signal marks enhancers, promoters, and transcription factor footprints across the genome.
The CUTANA™ ATAC-seq Kit pairs a highly active, pre-charged Tn5 Transposome with an optimized, Omni-ATAC–based workflow to deliver reproducible accessibility profiles from as few as 1,000 nuclei. Validated, competitor-benchmarked reagents and a streamlined, cells-to-libraries protocol give you high-quality data with less troubleshooting, at a low cost per reaction.
Resources:
- Tech Support Center: FAQs, troubleshooting, and protocol optimization guides in one convenient hub.
- Product Publications: Browse CUTANA citations to see how researchers apply our reagents.
Shop ATAC-seq products below or Request a Quote to get started. Prefer to have our experts run the assay? Explore our CUTANA™ ATAC-seq Services for full-service execution and data delivery.
ATAC-seq Workflow
The CUTANA™ ATAC-seq workflow takes you from cells or isolated nuclei to sequencing-ready libraries in a single afternoon. Isolated nuclei are combined with pre-loaded CUTANA™ Tn5 Transposome, which simultaneously cuts accessible chromatin and inserts sequencing adapters in one tagmentation step, the reaction that gives ATAC-seq its speed and simplicity. After tagmentation is stopped, the tagmented DNA is cleaned up with SPRI-based DNA purification beads (no spin columns, no pH steps), amplified and uniquely indexed by PCR using dual-index primers, and given a second SPRI cleanup to remove primers and adapter dimers. The finished libraries are quantified and pooled for standard Illumina paired-end sequencing. Because tagmentation, cleanup, and indexing are all optimized to work together, the workflow produces consistent, high-signal accessibility profiles across a wide range of inputs, down to 1,000 nuclei.
Competitor Benchmarking Data
Figure 1. CUTANA™ ATAC-seq Kit outperforms a leading competitor kit at low input
ATAC-seq performed on K562 nuclei using the CUTANA™ ATAC-seq Kit or Competitor A’s kit across an input titration from 50,000 to 1,000 nuclei. The assay shows the CUTANA™ Kit maintains high-quality data at every input, while Competitor A declines sharply at low input. (A) Genome browser tracks at high- (GAPDH, 15 kb), medium- (VAMP2, 11 kb), and low-expression (SLC12A5, 35 kb) loci, expression tiers set by RNA-seq, show maintained peak signal with the CUTANA™ Kit across 50k, 10k, and 1k nuclei, whereas Competitor A weakens progressively and is minimal at 1k. (B) TSS enrichment (±2 kb, 23,235 genes) stays strong across all inputs with the CUTANA™ Kit, while Competitor A drops sharply and is minimal by 1k nuclei.
Figure 2. CUTANA™ Tn5 performance is comparable to a leading competitor enzyme
ATAC-seq was performed on K562 nuclei using either CUTANA™ Tn5 Transposome or Competitor B Transposome, as part of the CUTANA™ ATAC-seq Kit. The assay yields comparable high-quality data at 50k and 10k nuclei. (A) Genome browser tracks at high- (GAPDH, 15 kb), medium- (VAMP2, 11 kb), and low-expression (SLC12A5, 35 kb) loci, expression tiers set by RNA-seq, show comparable peak position and shape for both transposomes at both inputs. (B) TSS enrichment (±2 kb, 23,235 genes) is comparable between transposomes at 50k and 10k nuclei. (C) At a merged peak set from both transposomes at 50k nuclei (67,277 peaks, ±2 kb), signal is comparable, with the CUTANA™ Tn5 Transposome showing slightly higher overall signal.
| SKU | Name | Price | ||
|---|---|---|---|---|
 | 14-1121-24rxn, 14-1190-24rxn | CUTANA™ ATAC-seq Kit | Price range: $95.00 through $995.00 | |
 | 14-1190-24rxn | CUTANA™ Nextera-Compatible CDI Primer Set 1 | $95.00 | |
 | 15-1036 | CUTANA™ Tn5 Transposome | Price range: $245.00 through $3,575.00 |