SKU: 19-5002
{"url":"https://www.epicypher.com/products/nucleosomes/snap-cutana-ha-tag-panel","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":1138,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"2 barcoded spike-in nucleosomes provide quantitative sample normalization and confirmation of HA antibody specificity in CUT&RUN","category":["Nucleosomes","Nucleosomes/SNAP-CUTANA™ Spike-in Controls","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1138/1209/CUTANAspike-inthumbnail_RGB_white_w_border__32266.1707343644.png?c=2","alt":"SNAP-CUTANA™ HA Tag Panel"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=1138","shipping":{"calculated":true},"num_reviews":0,"weight":"0.00 LBS","custom_fields":[{"id":"1298","name":"Pack Size","value":"50 Reactions"},{"id":"1299","name":"Internal Comment","value":"Excess in bottom shelf of Venom."}],"sku":"19-5002","description":"<!-- <div class=\"product-general-info\">\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div id=\"w-s-3835-19-1002\" style=\"\n width: 240px;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-19-1002-div\" style=\"width: 240px\">\n <a id=\"bioz-w-pb-19-1002\" style=\"font-size: 12px; color: transparent\" href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n Powered by Bioz\n </a>\n </div>\n </a>\n </li>\n </ul>\n</div> -->\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n The SNAP-CUTANA™ HA Tag Panel of spike-in controls for CUT&RUN offers an in-assay control to validate\n anti-HA antibodies and confirm the success of CUT&RUN reactions involving HA epitope-tagged chromatin\n proteins.\n This essential positive control guides troubleshooting to differentiate problems with HA epitope-tagging\n (including transgene expression, chromatin binding of the tagged protein, solvent accessibility of the tag,\n etc.) from technical failures in the CUT&RUN workflow. The panel consists of two nucleosomes containing\n unmodified histone H3 or 3xHA-H3 fusion, each wrapped with two uniquely barcoded DNA templates (A and B, for\n an internal technical replicate). The nucleosomes are individually conjugated to paramagnetic beads and\n pooled into a single panel for convenient one-step spike-in to CUT&RUN reactions. The panel is added\n alongside ConA-immobilized cells just prior to the addition of anti-HA or IgG negative control antibodies\n (see <strong>Application Notes</strong> and <strong>Table 1</strong>). The release of genomic chromatin and\n the barcoded nucleosomes by\n pAG-MNase is dependent on the specificity of the antibody used. After sequencing, the relative read count of\n recovered HA vs. unmodified nucleosomes provides a quantitative metric of on- vs. off-target recovery\n (<strong>Figure 2</strong>), thereby gauging experimental success and guiding troubleshooting efforts. See\n the most recent <a href=\"/resources/protocols/cutana-cut-and-run-kit-manual\" target=\"_blank\">CUTANA™ CUT&RUN\n protocol</a> and <a href=\"/resources/protocols/\" target=\"_blank\">SNAP-CUTANA™ Spike-in User Guide</a> for\n detailed information on workflow integration, expected results, data analysis, and troubleshooting.\n </p>\n <p style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n \">\n <a target=\"_blank\" style=\"color: #fff;\"\n href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ha-tag-cutana-cut-and-run-antibody\">Pair\n with our HA Tag CUTANA™ CUT&RUN Antibody for CUT&RUN success. Check it out here.\n </a>\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/19-5002_graphic.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"19-5002_graphic\"\n src=\"/content/images/products/nucleosomes/19-5002_graphic.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: Schematic of SNAP-CUTANA™ HA Tag\n Panel</strong><br />\n The HA Tag Panel contains two nucleosomes - one has an H3 tail fusion to a 3xHA Tag epitope and one\n is an unmodified control. Both octamers are wrapped with two uniquely barcoded DNA templates (A and\n B). Each 250 bp DNA template contains a 123 bp 601 nucleosome positioning sequence (gray) [1], a\n unique 22 bp DNA-barcode (white; 4 barcodes total), and a 5’ biotin-TEG. The 5’ and 3’ linkers\n (blue) are compatible with cleavage by pAG-MNase (EpiCypher <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n target=\"_blank\">14-1048</a>, <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows\"\n target=\"_blank\">15-1016</a>) during CUT&RUN. The\n nucleosomes are individually pre-conjugated to paramagnetic beads and pooled for convenient use.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/19-5002_ha_tag_panel.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"19-5002_ha_tag_panel\"\n src=\"/content/images/products/nucleosomes/19-5002_ha_tag_panel.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: SNAP-CUTANA™ HA Tag Panel provides an\n in-assay control for CUT&RUN reactions targeting HA-tagged proteins</strong><br />\n CUT&RUN was performed as described in <strong>Figure 5</strong>. CUT&RUN sequencing reads were\n aligned to the\n unique DNA\n barcodes corresponding to each nucleosome in the SNAP-CUTANA™ HA Tag Panel. Data are expressed as a\n percent relative to on-target recovery (HA Tag set to 100%) or total counts (IgG). IgG antibody\n results demonstrate equal loading of unmodified and epitope nucleosomes in the panel. HA Tag\n antibody results show selective enrichment of the HA Tag spike-in nucleosomes, validating all\n CUT&RUN steps, including HA antibody binding, pAG-MNase cleavage, and wash conditions\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/19-5002_table.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\">\n <img loading=\"lazy\" alt=\"19-5002_table\"\n src=\"/content/images/products/nucleosomes/19-5002_table.jpeg\" class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Table 1: Recommended SNAP-CUTANA™ HA Tag Panel Spike-in dilution for CUT&RUN reactions of\n varying starting cell number.</strong><br />\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/19-5002_dna_gel_data.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\">\n <img loading=\"lazy\" alt=\"19-5002_dna_gel_data\"\n src=\"/content/images/products/nucleosomes/19-5002_dna_gel_data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: DNA gel data</strong><br />\n Nucleosomes in the SNAP-CUTANA™ HA Tag Panel were resolved via native PAGE and stained with ethidium\n bromide to confirm intact nucleosome assembly. <strong>Lane 1:</strong> Free\n 250 bp DNA used in\n nucleosome assembly (100 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/19-5002_protein_gel_data.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\">\n <img loading=\"lazy\" alt=\"19-5002_protein_gel_data\"\n src=\"/content/images/products/nucleosomes/19-5002_protein_gel_data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 4: Protein gel data</strong><br />\n Coomassie stained SDS-PAGE gel of the nucleosome containing a 3xHA-H3 fusion (1 μg) in the\n SNAP-CUTANA™\n HA Tag Panel demonstrates the purity of histones in the preparation. Sizes of molecular weight\n markers and positions of the core histones (H2A, H2B, 3xHA-H3, and H4) are indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/nucleosomes/19-5002_cr_workflow.png\" target=\"_blank\"\n class=\"image-picker__main-image-link\">\n <img loading=\"lazy\" alt=\"19-5002_cr_workflow\"\n src=\"/content/images/products/nucleosomes/19-5002_cr_workflow.png\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span>\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 5: CUT&RUN methods</strong><br />\n CUT&RUN was performed on 500k MDA-MB-231 native cells stably expressing 3xHA-tagged GATA3 [1]*\n using the CUTANA™ ChIC/CUT&RUN Kit v3 (EpiCypher <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n target=\"_blank\">14-1048</a>). SNAP-CUTANA™ HA Tag Panel was added just\n prior to the addition of either HA Tag (0.5 µg; EpiCypher <a\n href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ha-tag-cutana-cut-and-run-antibody\"\n target=\"_blank\">13-2010</a>) or IgG negative control (0.5 µg;\n EpiCypher <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-igg-negative-control-antibody-for-cut-run-and-cut-tag\"\n target=\"_blank\">13-0042</a>) antibodies. Library preparation was performed with 5 ng of DNA (or\n the total\n amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher\n <a href=\"/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit\"\n target=\"_blank\">14-1001/14-1002</a>). Libraries were run on an Illumina NextSeq2000 with\n paired-end sequencing (2x50\n bp). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates,\n multi-aligned reads, and ENCODE DAC Exclusion List regions.\n <br>\n <br>\n <i>*Thanks to Dr. Takaku (UND) for 3xFLAG-GATA3-3xHA MDA-MB-231 cells.</i>\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\" alt=\"19-5002_graphic\"\n src=\"/content/images/products/nucleosomes/19-5002_graphic.jpeg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" />\n <img loading=\"lazy\" alt=\"19-5002_ha_tag_panel\"\n src=\"/content/images/products/nucleosomes/19-5002_ha_tag_panel.jpeg\" class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\" alt=\"19-5002_table\" src=\"/content/images/products/nucleosomes/19-5002_table.jpeg\"\n class=\"image-picker__side-image\" role=\"button\" />\n <img loading=\"lazy\" alt=\"19-5002_dna_gel_data\"\n src=\"/content/images/products/nucleosomes/19-5002_dna_gel_data.jpeg\" class=\"image-picker__side-image\"\n role=\"button\" />\n <img loading=\"lazy\" alt=\"19-5002_protein_gel_data\"\n src=\"/content/images/products/nucleosomes/19-5002_protein_gel_data.jpeg\"\n class=\"image-picker__side-image\" role=\"button\" />\n <img loading=\"lazy\" alt=\"19-5002_cr_workflow\"\n src=\"/content/images/products/nucleosomes/19-5002_cr_workflow.png\" class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"userguide\"></div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Store at -20°C.\n <strong>Lower temperatures can cause freezing and will\n permanently damage the magnetic beads</strong>. Stable for six months from date of\n receipt.<br />\n To resuspend beads, gently mix into an even suspension by pipetting; <strong>DO NOT VORTEX</strong>\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A mixture of two semi-synthetic nucleosomes conjugated to paramagnetic beads in 10 mM sodium\n cacodylate pH 7.5, 100 mM NaCl, 1 mM EDTA, 50% glycerol (w/v), 1x Protease Inhibitor Cocktail,\n 100 μg/mL BSA, 10 mM β-mercaptoethanol.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n <em>See the most recent <a href=\"/resources/protocols/cutana-cut-and-run-kit-manual\" target=\"_blank\">CUTANA™ CUT&RUN\n protocol</a> and <a href=\"/resources/protocols/\" target=\"_blank\">SNAP-CUTANA™ Spike-in User Guide</a>\n for detailed information on workflow integration, expected results, data analysis, and troubleshooting. In\n Brief:</em>\n </p>\n <p>\n <u><strong>Product Use:</strong></u> Use the SNAP-CUTANA™ HA Tag Panel for reactions containing <a href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ha-tag-cutana-cut-and-run-antibody\" target=\"_blank\">HA</a> and <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-igg-negative-control-antibody-for-cut-run-and-cut-tag\">IgG</a>\n antibodies. Just before antibody addition in CUT&RUN, gently pipette to resuspend beads (do\n not vortex), then spike in 2 μL per 500k cells. If using less than the standard number of cells, decrease\n the amount of SNAP-CUTANA spike-in linearly by preparing a “working stock” dilution of the panel in Antibody\n Buffer, made fresh the day of use (<strong>Table 1</strong>). Adjust spike-in volume as needed aiming for\n the spike-in\n barcodes to comprise ~1% of the total unique sequencing reads. Table 1 gives recommended dilution amounts\n for varying numbers of starting cells, but optimization may be required for user-specific conditions.\n </p>\n\n <p>\n <u><strong>Data Analysis:</strong></u> Detailed instructions are in the SNAP-CUTANA™ Spike-in User Guide (see <strong>Documents & Resources</strong> section below). Perform paired-end sequencing for a minimum of 50 bases. The Widom\n 601 DNA and DNA barcodes are distinct from human, mouse, fly, and yeast genomes such that they can be\n readily distinguished from sample chromatin. A shell script (.sh file extension) for spike-in alignment and\n an excel template for heatmap generation are available in the <strong>Documents & Resources</strong> section\n below. The shell\n script can be opened with any basic text editor program and contains detailed instructions hashed (#) at the\n beginning of the document. Make sure to copy and paste the R1 & R2 echo loop so there is a set for each\n reaction being analyzed.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID:\n <a href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n title=\"New DNA Sequence Rules for High Affinity Binding to Histone Octamer and Sequence-Directed Nucleosome Positioning\"\n target=\"_blank\">9514715</a><br />\n [2] Takaku et al. <em>Genome Biol.</em> (2016). PMID:\n <a href=\"https://pubmed.ncbi.nlm.nih.gov/26922637/\"\n title=\"Expression and Purification of Recombinant Histones and Nucleosome Reconstitution\" target=\"_blank\">\n 26922637</a><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/19-5002.pdf\" target=\"_blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"19-5002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n 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class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n 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href=\"/content/documents/SNAP-CUTANA_HA_Tag_Panel_Analysis.xlsx\" target=\"_blank\"\n class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\">\n <g>\n <path class=\"product-documents__svg-excel\"\n d=\"M193.17,69.88l-46.83-46.83c-1.31-1.31-3.07-2.05-4.92-2.05H48.96C45.11,21,42,24.11,42,27.96v181.07\n c0,3.85,3.11,6.96,6.96,6.96h139.29c3.85,0,6.96-3.11,6.96-6.96V74.82C195.21,72.97,194.47,71.19,193.17,69.88L193.17,69.88z\n M179.15,78.02h-40.96V37.06L179.15,78.02z M179.54,200.33H57.67V36.67h65.73v47.01c0,5.05,4.09,9.14,9.14,9.14h47.01V200.33z\n M119.06,133.32l-13.45-22.29c-0.48-0.78-2.24-1.24-2.24-1.24h-8.33c-0.5,0-0.98,0.13-1.39,0.41c-1.21,0.76-1.58,2.36-0.8,3.6\n 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Pack Size: 50 Reactions
