EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated

$565.00

SKU: 16-4112
{"url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-2-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":656,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2 for Chromatin Remodeling Assays","category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Discontinued Products"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/656/971/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc12-biotinylated__56258.1645734563.jpg?c=2","alt":"EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=656","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"576","name":"Pack Size","value":"50 µg"}],"sku":"16-4112","description":"<body>\n\n\n<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody>\n\n<tr valign=\"top\"> \n\n<td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> \n\n\n\n<tr> <td valign=\"top\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 217 base pair DNA sequence that includes the Widom 601 sequence with two added GATC sequences and a 5' biotin-TEG group. \n \n \n\n </td> \n</tr> <tr> \n \n \n \n \n \n \n \n \n \n \n <td valign=\"top\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Formulation: Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 243,101.5 Da. </td> \n </tr> \n \n \n \n \n \n \n \n \n \n <tr> <td valign=\"top\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. </td> </tr> <tr> <td valign=\"top\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Application Notes: This product is a template for nucleosome remodeling assays using the restriction enzyme DpnII to determine accessibility of GATCs which is masked in its native configuration (prior to remodeling). DNA Sequence: GAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGATCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTT\nAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGATGGATGATGA\nTGGATGAATAGATGGATGGATGAAGCTT </td> </tr> <tr> <td valign=\"top\">References: \n </td> </tr> \n \n <tr> <td valign=\"top\"> View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-4112.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-4112 Datasheet\" width=\"30\" height=\"40\" /></a> </td> </tr> \n \n <tr> <td valign=\"top\"> View Restriction Enzyme Assay application technical note for this product. <a href=\"https://www.epicypher.com/content/documents/technotes/EpiDyne_REA.pdf\" target=\"_blank\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-4112 Tech Note\" width=\"30\" height=\"40\" /></a> </td> </tr> \n \n \n </tbody> </table> </td> \n \n \n \n <td width=\"350px\"> <table style=\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> \n \n \n <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4112_DNA_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4112_DNA_gel_tds.jpg\" alt=\"16-4112 DNAGel\" width=\"150\"/></a></td> </tr> <tr> <td align=\"left\" valign=\"top\">DNA Gel Data: Free DNA (Lane 1, 100 ng) and EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated (Lane 2, 400 ng) resolved via native page and stained with ethidium bromide to visualize DNA. </td> \n </tr> \n \n \n <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4112_protein_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4112_protein_gel_tds.jpg\" alt=\"16-4112 Protein Gel\" width=\"200\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\">Protein Gel Data: Histone proteins in EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated resolved via SDS-PAGE and coomassie staining (2 ug). Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated. </td> \n </tr> \n \n \n \n \n <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4112_remodel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4112_remodel_tds.jpg\" alt=\"16-4112 REA Gel\" width=\"250\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\">Nucleosome Remodeling Data: ACF/ATP-dependent nucleosome remodeling reaction in the presence of DpnII restriction enzyme. EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated nucleosomes incubated with (10nM) or without ACF for up to 20 minutes in the presence of 2 mM ATP and 50U of DpnII. Samples were quenched at specified intervals and run on 8% native PAGE gel.</td> \n </tr> \n \n \n \n \n \n \n </tbody> </table> </td> </tr> </tbody> </table> </div>\n\n \n\n\n\n</body>\n</html>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$565.00","value":565,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":658,"sku":"18-4112","name":"EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-remodeling-assay-substrate-dna-st601-gatc1-2-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"summary":" EpiDyne Remode","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/658/947/epidyne-remodeling-assay-substrate-dna-st601-gatc12-biotinylated__78976.1645734539.jpg?c=2","alt":"EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/658/947/epidyne-remodeling-assay-substrate-dna-st601-gatc12-biotinylated__78976.1645734539.jpg?c=2","alt":"EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated"}],"date_added":"25th Feb 2019","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":577,"name":"Pack Size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 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EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66, Biotinylated Description: ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/725/813/2021.02.25_EPI_Nucleosome_thumbnail_only_50N66_white_background_RBG_AL_MP__28000.1620836268.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/725/813/2021.02.25_EPI_Nucleosome_thumbnail_only_50N66_white_background_RBG_AL_MP__28000.1620836268.png?c=2","alt":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66, Biotinylated"},{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/725/929/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc12-50-n-66-biotinylated__58827.1645734520.jpg?c=2","alt":"EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66, Biotinylated"}],"date_added":"9th Jan 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EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 217 base pair DNA sequence that includes the Widom 601 sequence with two added GATC sequences and a 5' biotin-TEG group.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Formulation: Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 243,101.5 Da.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated Application Notes: This product is a template for nucleosome remodeling assays using the restriction enzyme DpnII to determine accessibility of GATCs which is masked in its native configuration (prior to remodeling). DNA Sequence: GAATTCATCAGAATCCCGGTGCCGAGGCCGATCAATTGATCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTT AACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCGATGATGATGGATAGATGGATGATGGATGGATGGATGATGA TGGATGAATAGATGGATGGATGAAGCTT

References:

View technical datasheet for this product.

View Restriction Enzyme Assay application technical note for this product.

DNA Gel Data: Free DNA (Lane 1, 100 ng) and EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated (Lane 2, 400 ng) resolved via native page and stained with ethidium bromide to visualize DNA.

Protein Gel Data: Histone proteins in EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated resolved via SDS-PAGE and coomassie staining (2 ug). Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.

Nucleosome Remodeling Data: ACF/ATP-dependent nucleosome remodeling reaction in the presence of DpnII restriction enzyme. EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated nucleosomes incubated with (10nM) or without ACF for up to 20 minutes in the presence of 2 mM ATP and 50U of DpnII. Samples were quenched at specified intervals and run on 8% native PAGE gel.

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