Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated

$575.00

SKU: 16-2144
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","category":["Nucleosomes","Nucleosomes/Methyl DNA Designer Nucleosomes"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/793/767/Long_tail_non_methyl_DNA__31800.1532696948.1280.1280__64910.1603296544.1280.1280__90181.1606315313.png?c=2","alt":"Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=793","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1103","name":"Pack size","value":"50 µg"},{"id":"1104","name":"Internal Comment","value":"21 units of lot 2 in New Lot Rack in Psylocke, included in count"}],"sku":"16-2144","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"><tbody><tr valign=\"top\"><td><table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr>\n <td valign=\"top\">Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated Description: Mononucleosomes assembled from recombinant histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P06897; H2B-P02281; H3-Q92133; H4-P62799) wrapped by 199 base pairs of DNA containing the 601 positioning sequence DNA. The the 199 bp DNA sequence contains a 147 base-pair 601 nucleosome positioning sequence. The 601 sequence is flanked by a 26 bp sequence as shown in application notes. </td></tr><tr>\n <td valign=\"top\">Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated Formulation: Mononucleosomes, Recombinant, 199x601 DNA (50 µg DNA + protein, 24.3 µg protein weight) in 10 mM Tris pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 231,289.05 Da. </td></tr><tr><td valign=\"top\">Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. </td></tr><tr><td valign=\"top\">Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated Application Notes: \n \n \n \n \n5'-GGACCCTATACGCGGCCGCCGAATTCCTGGAGAATCCCG \n GTCTGCAGGCCGCTCAATTGGTCGTAGACAGCTCTACGTGGC \n GAATTTGCGTGCATGCGCCTGTCCCCCGCGTTTTAACCGCCA \n AGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATAC \n ATCCTGTGGATCCGCCGGTCGCGAACAGCGACC-3'\n\n3'-CCTGGGATATGCGCCGGCGGCTTAAGGACCTCTTAGGGCCAGACGT \nCCGGCGAGTTAACCAGCATCTGTCGAGATGCACCGCTTAAAC \nGCACGTACGCGGACAGGGGGCGCAAAATTGGCGGTTCCCCT \nAATGAGGGATCAGAGGTCCGTGCACAGTCTATATATGTAGGACA \nCCTAGGCGGCAGCGCTTGTCGCTGG-5' \n\n\n\n\n\n\n <tr><td valign=\"top\">References: \n </td></tr><tr><td valign=\"top\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-2144.pdf\" target=\"-blank\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-2144 Datasheet\" width=\"30\" height=\"40\" /></a></td></tr></tbody></table></td>\n \n \n \n \n <td><table width=\"300\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody>\n \n \n \n\n \n \n \n \n <tr valign=\"top\"><td valign=\"top\" align=\"center\"><a href=\"/content/images/products/nucleosomes/16-2144_SDS_PAGE.jpg\" target=\"_new\"> <img src=\"/content/images/products/nucleosomes/16-2144_SDS_PAGE.jpg\" alt=\"16-2144 Protein Gel\" width=\"125\" height=\"300\" /></a></td></tr>\n \n <tr>\n <td valign=\"top\" align=\"left\">Protein Gel Data: Coomassie stained PAGE gel of proteins in Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated (0.75 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated. \n (Click image to enlarge) </td></tr>\n \n \n \n \n \n <tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-2144_Native_PAGE.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-2144_Native_PAGE.jpg\" alt=\"16-2144 DNA Gel\" width=\"125\" height=\"300\" /></a></td></tr>\n \n \n \n <tr>\n <td align=\"left\" valign=\"top\">DNA Gel Data: Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated run on a native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA. Lane 2: Intact nucleosomes (200 ng). \n (Click image to enlarge) </td></tr> \n\n \n \n \n \n \n \n \n </tbody></table></td></tr>\n \n \n </tbody></table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Unmethylated DNA Recombinant Nucleosome","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[{"id":1150,"type":"Configurable_PickList_Set","display_name":"Select Pack Size","required":true,"condition":false,"state":"modifier","values":[{"label":"50 µg","id":81,"data":"50 µg","selected":true}],"partial":"set-radio"}],"related_products":[{"id":792,"sku":"16-2044","name":"Mononucleosomes, Recombinant, 199x601 DNA, 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Mon","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/790/764/Long_tail_hemi-methyl_DNA__92953.1525791282.1280.1280__73777.1603294945.gif?c=2","alt":"Mononucleosomes, Recombinant, Hemi-methylated 199x601 DNA, Non-biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/790/764/Long_tail_hemi-methyl_DNA__92953.1525791282.1280.1280__73777.1603294945.gif?c=2","alt":"Mononucleosomes, Recombinant, Hemi-methylated 199x601 DNA, Non-biotinylated"}],"date_added":"21st Oct 2020","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":638,"name":"Pack size","value":"50 µg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales 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methylation, methyl reader, DNA methyltransferase, recombinant nucleosome, dNuc","show_quantity_input":1,"title":"Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/793/767/Long_tail_non_methyl_DNA__31800.1532696948.1280.1280__64910.1603296544.1280.1280__90181.1606315313.png?c=2","alt":"Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated"}]} Pack size: 50 µg

Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated Description: Mononucleosomes assembled from recombinant histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P06897; H2B-P02281; H3-Q92133; H4-P62799) wrapped by 199 base pairs of DNA containing the 601 positioning sequence DNA. The the 199 bp DNA sequence contains a 147 base-pair 601 nucleosome positioning sequence. The 601 sequence is flanked by a 26 bp sequence as shown in application notes.

Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated Formulation: Mononucleosomes, Recombinant, 199x601 DNA (50 µg DNA + protein, 24.3 µg protein weight) in 10 mM Tris pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 231,289.05 Da.

Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated Storage and Stability: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated Application Notes:

5'-GGACCCTATACGCGGCCGCCGAATTCCTGGAGAATCCCG
GTCTGCAGGCCGCTCAATTGGTCGTAGACAGCTCTACGTGGC
GAATTTGCGTGCATGCGCCTGTCCCCCGCGTTTTAACCGCCA
AGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATAC
ATCCTGTGGATCCGCCGGTCGCGAACAGCGACC-3'

3'-CCTGGGATATGCGCCGGCGGCTTAAGGACCTCTTAGGGCCAGACGT
CCGGCGAGTTAACCAGCATCTGTCGAGATGCACCGCTTAAAC
GCACGTACGCGGACAGGGGGCGCAAAATTGGCGGTTCCCCT
AATGAGGGATCAGAGGTCCGTGCACAGTCTATATATGTAGGACA
CCTAGGCGGCAGCGCTTGTCGCTGG-5'

References:

View technical datasheet for this product.

Protein Gel Data: Coomassie stained PAGE gel of proteins in Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated (0.75 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.
(Click image to enlarge)

DNA Gel Data: Mononucleosomes, Recombinant, 199x601 DNA, Non-biotinylated run on a native PAGE gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA. Lane 2: Intact nucleosomes (200 ng).
(Click image to enlarge)

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