SKU: 16-0395
{"url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h2ak119ub1-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":800,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Recombinant nucleosome with monoubiquitinylated histone H2A at lysine 119 offers a defined substrate for chromatin research and drug discovery.","category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/800/789/1591818618.1280.1280__68061.1607995447.1280.1280__45790.1614276469.jpg?c=2","alt":"Nucleosome, Recombinant Human, H2AK119ub1 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=800","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"660","name":"Pack Size","value":"25 µg"}],"sku":"16-0395","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H2AK119ub dNuc, Biotinylated Description:</b> Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A,\nH2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H2A (created by a proprietary semi-synthetic method) contains ubiquitin-lysine at position 119. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers, is useful for nucleosome assembly, and contains a 5’ biotin-TEG group.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H2AK119ub</b></span></span> dNuc Biotinylated Formulation:</b> Nucleosome, Recombinant Human, H2AK119ub (14.4 μg protein weight, 25 μg DNA+protein) in 10 mM Tris HCl,\npH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 216,953.5 Da.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H2AK119ub dNuc,\nBiotinylated Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For\nbest results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr>\n <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H2AK119ub dNuc,\nBiotinylated Application Notes:</b> Nucleosome, Recombinant Human, H2AK119ub dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. <br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b> Lowary PT and J Widom (1998). J Mol Biol 276: 19-42.\nLuger K et al (1999). Methods Mol Biol 119: 1-16.<br /> <br /></span></span><br /></p></td></tr>\n\n<tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0395.pdf\" target=\"_new\"><img loading=\"lazy\" src=\"/content/documents/tds/icon.png\" alt=\"16-0395 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td>\n\n<td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0395-WB.jpg\" target=\"_new\"><img loading=\"lazy\" src=\"/content/images/products/nucleosomes/16-0395-WB.jpg\" alt=\"16-0395 Western Blot Data\" width=\"125\" height=\"325\" /></a> </td></tr>\n\n<tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong>Western Analysis of Nucleosome, Recombinant Human, H2AK119ub. <strong>Top Panel:</strong> Unmodified H3\n(Lane 1) and H2AK119ub containing nucleosomes (Lane 2) were probed with an anti-H2AK119ub antibody and analyzed via ECL readout. Only the H2AK119ub sample produced a detectable signal. <strong> Bottom Panel:</strong> Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H2AK119ub nucleosomes (Lane 2).<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody>\n\t\n\t<td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0395-MS.jpg\" target=\"_new\"><img loading=\"lazy\" src=\"/content/images/products/nucleosomes/16-0395-MS.jpg\" alt=\"16-0395 Mass Spec\" width=\"125\" height=\"125\" /></a> </td></tr>\n\n<tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> H2AK119ub protein analyzed by high resolution mass spectrometry. Expected mass = 22,551 Da. Determined mass = 22,549.75 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody>\n\t\n\t<tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0395-SDS.jpg\" target=\"_new\"><img loading=\"lazy\" src=\"/content/images/products/nucleosomes/16-0395-SDS.jpg\" alt=\"16-0395 Protein Gel\" width=\"125\" height=\"325\" /></a></td></tr>\n\t\n\t<tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in\nNucleosome, Recombinant Human, H2AK119ub (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2AK119ub, H2B, H3.1, and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody>\n\t\n\t<tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0395-DNA.jpg\" target=\"_new\"><img loading=\"lazy\" src=\"/content/images/products/nucleosomes/16-0395-DNA.jpg\" alt=\"16-0395 Native PAGE Data\" width=\"125\" height=\"325\" /></a></td></tr>\n\t\n\t<tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H2AK119ub dNucs resolved via native PAGE and stained with ethidium\nbromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (100 ng). <strong>Lane 2:</strong> Intact H2AK119ub nucleosome (400 ng).<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> \n\n\n\n\n\n</tbody></table></td></tr>\n\n\n\n</tbody></table> </td></tr></tbody></table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$525.00","value":525,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H2AK119ub1 Recombinant Nucleosome (dNuc)","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[{"id":1162,"type":"Configurable_PickList_Set","display_name":"Select Pack Size","required":true,"condition":false,"state":"modifier","values":[{"label":"25 µg","id":88,"data":"25 µg","selected":true}],"partial":"set-select"}],"related_products":[{"id":605,"sku":"16-0358","name":"Nucleosome, Recombinant Human, H4R3me2s dNuc, 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Pack Size: 25 µg
