SKU: 16-0320
{"sku":"16-0320","url":"https://www.epicypher.com/products/featured-products/nucleosome-recombinant-human-h3k36me3-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[{"id":1098,"type":"Configurable_PickList_Set","display_name":"Select Pack Size:","required":true,"condition":true,"state":"variant_option","values":[{"label":"50 µg","id":117,"data":"50 µg","selected":true},{"label":"20 µg","id":118,"data":"20 µg","selected":false}],"partial":"set-radio"}],"id":322,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"H3 K36Me3 Recombinant Nucleosome (dNuc), Biotinylated, for Enzyme Screening Assays","category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)","Nucleosomes/Modified Designer Nucleosomes (dNucs™)/dNuc - Smaller Pack Size Available"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/322/310/Recombinant_nucleosome__33529.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K36me3 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=322","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong><em>E. coli</em> & synthetic DNA\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Tag: </strong>Biotinylated\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Molecular Weight: </strong>199,943 Da\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <p>\n Designer mononucleosomes (dNucs) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. Histone H3 (created by a proprietary synthetic method) contains trimethyl-lysine at position 36. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA in this nucleosome contains a 5’ biotin-TEG group.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-1.jpeg?t=1699859850\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0389-western-blot-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-1.jpeg?t=1699859850\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: Western blot data </strong><br />\n Western Analysis of H3K36me3 dNuc. <strong>Top Panel</strong>:\nUnmodified (EpiCypher <a href=\"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human-biotinylated\" target=\"_blank\">16-0006</a>; Lane 1) and H3K36me3 nucleosomes (Lane 2) were\nprobed with an anti-H3K36me3 antibody and analyzed via ECL readout. Only the\nH3K36me3 sample produced a detectable signal. <strong>Bottom Panel</strong>: Detail from\nCoomassie stained gel showing unmodified H3 (Lane 1) and H3K36me3 nucleosome\n(Lane 2).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-2.jpeg?t=1699859851\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0389-mass-spec-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-2.jpeg?t=1699859851\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Mass spec data </strong><br />\n Synthetic H3K36me3 histone analyzed by high resolution\nmass spectrometry. Expected mass = 15,314.97 Da. Determined mass = 15,314.95\nDa.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure3.jpeg?t=1699859852\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0389-protein-gel-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure3.jpeg?t=1699859852\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 3: Protein gel data</strong><br />\n Coomassie stained PAGE gel of proteins in H3K36me3\ndNuc (1 µg) demonstrates the purity of histones in the preparation. Sizes of molecular\nweight markers and positions of the core histones (H2A, H2B, H3K36me3 and H4) are\nindicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-4.jpeg?t=1699859853\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0389-dna-gel-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-4.jpeg?t=1699859853\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 4: DNA gel data</strong><br />\n H3K36me3 dNuc resolved via native PAGE and stained with\nethidium bromide to visualize DNA. <strong>Lane 1</strong>: Free DNA (EpiCypher <a href=\"https://www.epicypher.com/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\" target=\"_blank\">18-0005</a>; 100 ng).\n<strong>Lane 2</strong>: Intact H3K36me3 nucleosomes (400 ng).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"16-0389-western-blot-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-1.jpeg?t=1699859850\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"16-0389-mass-spec-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-2.jpeg?t=1699859851\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"16-0389-protein-gel-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure3.jpeg?t=1699859852\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n <img\n alt=\"16-0389-dna-gel-data\"\n src=\"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/original/image-manager/16-0320-figure-4.jpeg?t=1699859853\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\"\n />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM\nDTT, and 20% glycerol. (27.3 µg protein, 50 µg DNA + protein)\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <p>\n H3K36me3 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays,\nhigh-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural\nstudies, and in effector protein binding experiments.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin-bottom: 0\">\n H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)\n </p>\n <p style=\"margin-bottom: 0\">\n H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)\n </p>\n <p style=\"margin-bottom: 0\">H3.1 - P68431 (alt. names: H3, H3/a, H3/b, H3/c, H3/d)</p>\n <p>H4 - P62805</p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <strong>Background References:</strong>\n <br />\n [1] Lowary & Widom. J. Mol. Biol. (1998) PMID: \n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n target=\"new\"\n >\n 9514715</a\n >\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0320.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H3K36Me3 Recombinant Nucleosome (dNuc), Biotinylated, for Enzyme Screening Assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"designer nucleosome, dNuc, H3K36Me3, h3 K36 trimethyl, nucleosome, mononucleosome, dNuc, 16-0320","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H3K36me3 dNuc, Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/322/310/Recombinant_nucleosome__33529.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K36me3 dNuc, Biotinylated"}]}
