SKU: 16-0317
{"sku":"16-0317","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k27me3-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":297,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"H3 K27Me3 Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)","Nucleosomes/Modified Designer Nucleosomes (dNucs™)/dNuc - Smaller Pack Size Available"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/297/303/Recombinant_nucleosome__26312.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27me3 dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=297","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"><tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br /><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3 K27me3</b></span></span> dNuc Description:</b> Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains trimethyl-lysine at position 27. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.<br /><br /></span></span></td></tr><tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H3 K27me3</b></span></span> dNuc Formulation:</b> Nucleosome, Recombinant Human, H3 K27me3 <span class=\"Apple-style-span\" style=\"font-size: 12pt; \">(<span class=\"Apple-style-span\" style=\"font-size: 12pt; \">50 µg total mass, DNA+protein</span>, 27.3 µg protein weight)</span> in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 200,774.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3 K27me3 dNuc Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H3 K27me3 dNuc Application Notes:</b> Nucleosome, Recombinant Human, H3 K27me3 are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. <strong>Nucleosome, Recombinant Human, H3 K27me3 from EpiCypher does not contain free DNA which could alter assayed activities.</strong><br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0317.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"16-0317 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0317-WB-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0317-WB-data.jpg\" alt=\"16-0317 Western Blot\" height=\"125\" /></a> </td></tr><tr><td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong> Western Analysis of Nucleosome, Recombinant Human, H3 K27me3. <strong>Top Panel:</strong> Unmodified H3 (Lane 1) and H3 K27me3 containing nucleosomes (Lane 2) were probed with an anti-H3 K27me3 antibody and analyzed via ECL readout. Only the H3 K27me3 sample produced a detectable signal. <strong>Bottom Panel: </strong>Detail from Coomassie stained gel showing rH3 protein (Lane 1) and H3 K27me3 octamer (Lane 2).<br /> <strong>(Click image to enlarge) </strong></span></span></p></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0317-MS-data.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0317-MS-data.jpg\" alt=\"16-0317 MS Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> Synthetic H3 K27me3 protein analyzed by ESI-TOF mass spectrometry. Expected mass = 15,314.97 Da. Determined mass = 15,314.54 Da.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0317-protein-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0317-protein-gel.jpg\" alt=\"16-0317 Protein Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H3 K27me3 (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 K27me3 and H4) are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0317-DNA-gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0317-DNA-gel.jpg\" alt=\"16-0317 Protein Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H3 K27me3 run on an agarose gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1: </strong>Free DNA extracted from nucleosomes (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td></tr></tbody></table> </td></tr></tbody></table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H3K27Me3 Recombinant Nucleosome (dNuc), Biotinylated, for Enzyme Screening Assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[{"id":1097,"type":"Configurable_PickList_Set","display_name":"Select Pack Size:","required":true,"condition":true,"state":"variant_option","values":[{"label":"50 µg","id":117,"data":"50 µg","selected":true},{"label":"20 µg","id":118,"data":"20 µg","selected":false}],"partial":"set-radio"}],"related_products":[{"id":375,"sku":"16-0338","name":"Nucleosome, Recombinant Human, H3K27me1 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k27me1-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/375/301/Recombinant_nucleosome__47545.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27me1 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/375/301/Recombinant_nucleosome__47545.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27me1 dNuc, Biotinylated"}],"date_added":"1st Aug 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":203,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=375","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=375"},{"id":376,"sku":"16-0339","name":"Nucleosome, Recombinant Human, H3K27me2 dNuc, Biotinylated","url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h3k27me2-dnuc-biotinylated","availability":"","rating":null,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"summary":" \n","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/376/302/Recombinant_nucleosome__59688.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27me2 dNuc, Biotinylated"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/376/302/Recombinant_nucleosome__59688.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27me2 dNuc, Biotinylated"}],"date_added":"1st Aug 2017","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":204,"name":"Pack Size","value":"50 μg"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=376","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=376"}],"shipping_messages":[],"rating":0,"meta_keywords":"designer nucleosome, dNuc, dnucs, H3 K27Me3, h3 K27 trimethyl, nucleosome, mononucleosome, h3 lysine 27 trimethyl, 16-0317","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H3K27me3 dNuc, Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/297/303/Recombinant_nucleosome__26312.1582226112.png?c=2","alt":"Nucleosome, Recombinant Human, H3K27me3 dNuc, Biotinylated"}]}
