SKU: 16-0312
{"url":"https://www.epicypher.com/products/nucleosomes/nucleosome-recombinant-human-h4k12ac-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":298,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"H4 K12Ac Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","category":["Nucleosomes","Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/298/318/Recombinant_nucleosome__94348.1582226102.png?c=2","alt":"Nucleosome, Recombinant Human, H4K12Ac dNuc, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=298","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"774","name":"Pack Size","value":"50 μg"},{"id":"775","name":"Internal Comment","value":"Has Bulk - Not Included in Amount"}],"sku":"16-0312","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"><tbody><tr valign=\"top\"><td><table width=\"90%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"><tbody><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br /><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H4 K12Ac</b></span></span> dNuc Description:</b> Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H4 (created by a proprietary synthetic method) contains acetyl-lysine at position 12. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.<br /><br /></span></span></td></tr><tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>Nucleosome, Recombinant Human, H4 K12Ac dNuc</b></span></span> Formulation:</b> Nucleosome, Recombinant Human, H4 K12Ac <span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \">(<span class=\"Apple-style-span\" style=\"font-size: 12pt; \">50 µg total mass, DNA+protein</span>, 27.3 µg protein weight)</span> in</span> 10mM Tris HCl, pH 7.5, 25mM NaCl, 1mM EDTA, 2mM DTT, 20% glycerol. MW = 200,905 Da.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H4 K12Ac dNuc Storage and Stability:</b> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.<br /><br /></span></span></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Nucleosome, Recombinant Human, H4 K12Ac dNuc Application Notes:</b> Nucleosome, Recombinant Human, H4 K12Ac are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein (especially bromodomain) binding experiments. <strong>Nucleosome, Recombinant Human, H4 K12Ac from EpiCypher does not contain free DNA which could alter assayed activities.</strong><br /><br /></span></span></td></tr><tr><td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /></span></span><br /></p></td></tr><tr><td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0312.pdf\" target=\"_new\"><img src=\"/content/documents/tds/icon.png\" alt=\"15-1010 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></td></tr></tbody></table></td><td> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0312_WB_gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0312_WB_gel.jpg\" alt=\"16-0312 dNuc Mass Spec Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Western Blot Data:</strong> Western blot analysis of Nucleosome, Recombinant Human, H4 K12Ac. <strong>Top Panel</strong>: Unmodified recombinant H4 (Lane 1) and H4K12Ac containing nucleosomes (Lane 2) were probed with an anti-H4K12Ac antibody and detected via ECL. Only the H4K12Ac dNuc sample produced a detectable signal. <strong>Bottom Panel:</strong> Detail from Coomassie stained gel showing recombinant H4 protein (Lane 1) and all four histones from H4 K12Ac nucleosome (Lane 2).<br /><strong>(Click image to enlarge)</strong></span></span></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0312_MS.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0312_MS.jpg\" alt=\"16-0312 dNuc Mass Spec Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Mass Spec Data:</strong> Synthetic H4 K12Ac protein analyzed by ESI-TOF mass spectrometry. Expected mass = 15,314.97 Da. Determined mass = 15,314.5 Da.<br /> <strong>(Click image to enlarge)</strong></span></span></td></tr></tbody></table><table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0312_protein_gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0312_protein_gel.jpg\" alt=\"16-0312 dNuc Protein Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H4 K12Ac (1 µg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H4 K12Ac and H4) are indicated.<br /> <strong>(Click image to enlarge)</strong></span></span></td></tr></tbody></table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"><tbody><tr><td align=\"center\"><a href=\"/content/images/products/nucleosomes/16-0312_DNA_gel.jpg\" target=\"_new\"><img src=\"/content/images/products/nucleosomes/16-0312_DNA_gel.jpg\" alt=\"16-0312 dNuc DNA Gel Data\" height=\"125\" /></a></td></tr><tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>DNA Gel Data:</strong> Nucleosome, Recombinant Human, H4 K12Ac run on an agarose gel and stained with ethidium bromide to visualize DNA. <strong>Lane 1: </strong>Free DNA extracted from nucleosomes (200 ng). <strong>Lane 2:</strong> Intact nucleosomes (400 ng).<br /> <strong>(Click image to enlarge)</strong></span></span></td></tr></tbody></table> </td></tr></tbody></table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"H4K12 Acetyl Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"designer nucleosome, dNuc, H4K12Ac, h4 K12 acetyl, nucleosome, mononucleosome, h4 acetyl, 16-0312","show_quantity_input":1,"title":"Nucleosome, Recombinant Human, H4K12Ac dNuc, Biotinylated","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/298/318/Recombinant_nucleosome__94348.1582226102.png?c=2","alt":"Nucleosome, Recombinant Human, H4K12Ac dNuc, Biotinylated"}]}
Pack Size: 50 μg
