SKU: 16-0003
{"url":"https://www.epicypher.com/products/nucleosomes/hela-polynucleosomes-purified","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":98,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Purified HeLa polynucleosomes for enzyme screening assays and chromatin binding experiments","category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/98/335/Human_recombinant_nucleosome__70596.1516390310.gif?c=2","alt":"HeLa Polynucleosomes, Purified"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=98","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"873","name":"Pack Size","value":"50 μg"},{"id":"874","name":"Internal Comment","value":"has bulk in LG"}],"sku":"16-0003","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Species: </strong>Human\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source: </strong>HeLa cells\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Tag: </strong>N/A\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Molecular Weight: </strong>230,000 Da\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n Human polynucleosomes purified from HeLa cells. The nucleosome is\n the basic subunit of chromatin, which consists of 150 base pairs of\n DNA wrapped around an octamer core of histone proteins (two each of\n H2A, H2B, H3 and H4). HeLa Polynucleosomes are predominantly\n trimers, with some dimers and tetramers (<strong>Figure 1</strong>).\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0003-dna-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0003-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0003-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA gel data </strong><br />\n DNA (2 µg) was purified from HeLa Polynucleosomes and\n resolved via agarose gel to show the size of nucleosomal DNA\n compared to molecular weight markers (base pairs). Dimers\n resolve at ~350 bp, trimers at 475 bp, and tetramers at ~625\n bp.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0003-protein-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"16-0003-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0003-protein-gel-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 2: Protein gel data </strong><br />\n Coomassie stained SDS-PAGE gel of proteins in HeLa\n Polynucleosomes (2 µg) demonstrates the purity of histones\n in the preparation. Sizes of molecular weight markers and\n position of the core histones (H2A, H2B, H3, and H4) are\n indicated.\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/16-0003-enzyme-activity-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\">\n <img\n alt=\"16-0003-enzyme-activity-data\"\n src=\"/content/images/products/nucleosomes/16-0003-enzyme-activity-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Enzyme activity data </strong><br />\n HMTase assay with HeLa Polynucleosomes (1 µg) and SET8 (HeLa\n Nucs + SET8; 1 µg) using a standard radiometric filter\n binding assay protocol. Controls include the Polynucleosomes\n alone (HeLa Nucs) or SET8 alone (SET8). SET8 is aa 1-352 of\n UniProt# Q9NQR1.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n alt=\"16-0003-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0003-dna-gel-data.jpeg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <img\n alt=\"16-0003-protein-gel-data\"\n src=\"/content/images/products/nucleosomes/16-0003-protein-gel-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n alt=\"16-0003-enzyme-activity-data\"\n src=\"/content/images/products/nucleosomes/16-0003-enzyme-activity-data.jpeg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for six months at -80°C from date of receipt. For best\n results, aliquot and avoid freeze/thaws\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 20 mM HEPES pH 7.5, 1 mM EDTA (25.2 µg protein, 50 µg DNA +\n protein)\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n HeLa Polynucleosomes are suitable for use in enzyme assays such as\n acetylation or methylation, chromatin binding, or as a positive\n control in western blotting. Use 1-2 µg per reaction.\n </p>\n <p>\n Molarity and molecular weight are estimated based on DNA size and\n account for the endogenous post-translational histone modifications.\n </p>\n <p>\n Note: despite the high purity of the preparation, a small amount of\n arginine methyltransferase (RMTase) activity may co-purify with the\n nucleosomes. If you are studying weak or low turnover HMTases, you\n can use AMI-1 to inhibit the endogenous RMTase activity.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Gene & Protein Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>UniProt ID</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a,\n H2A/m)<br />\n H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)<br />\n H3 - P68431, Q71DI3, K7EMV3<br />\n H4 - P62805\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <strong>Background References:</strong>\n <br />\n [1] Kuo et al. <em>Mol. Cell</em> (2011). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/22099308/\"\n target=\"_blank\"\n title=\"NSD2 links dimethylation of histone H3 at lysine 36 to oncogenic programming\"\n >22099308</a\n ><br />\n [2] Matthews et al. <em>Nature</em> (2007). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/18033247/\"\n target=\"_blank\"\n title=\"RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination\"\n >18033247</a\n ><br />\n [3] Shi et al. <em>Nature</em> (2006). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/16728974/\"\n target=\"_blank\"\n title=\"ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression\"\n >16728974</a\n ><br />\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/16-0003.pdf\"\n target=\"_blank\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0003 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$320.00","value":320,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Purified HeLa Polynucleosomes","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":"50 µg","shipping_messages":[],"rating":0,"meta_keywords":"HeLa polynucleosomes, hela nucleosomes, enzyme assays, enzyme screening, enzyme kinetics, acetylation, methylation, chromatin binding assays, Western blotting, chromatin, epigenetics","show_quantity_input":1,"title":"HeLa Polynucleosomes, Purified","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/98/335/Human_recombinant_nucleosome__70596.1516390310.gif?c=2","alt":"HeLa Polynucleosomes, Purified"}]}
Pack Size: 50 μg
