CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag

$145.00

SKU: 15-1018
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-tag-assays/cutana-non-hot-start-2x-pcr-master-mix-for-cut-tag","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":753,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"Non-Hot Start 2X PCR Master Mix for CUT&Tag / ChIC assays","category":["Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/753/752/DNA_Vector_Draw_Final-w-border-01__76823.1591894876.1280.1280__20924.1594475393.jpg?c=2","alt":"CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=753","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1068","name":"Pack Size","value":"50 reactions"},{"id":"1069","name":"Internal Comment","value":"extra in bottom of Venom"}],"sku":"15-1018","description":"<div class=\"product-general-info\">\n <ul class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\">\n <strong>Type:  </strong>Polymerase\n </li>\n <li class=\"product-general-info__list-item\">\n <strong>Source:  </strong><em>E. coli</em>\n </li>\n </ul>\n <ul class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <strong>Epitope Tag:  </strong>None\n </li>\n </ul>\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div id=\"w-s-3835-15-1018\" style=\"width: 240px; height: 58px; position: relative; overflow-y: hidden\"></div>\n \n </a>\n </li>\n </ul>\n</div>\n\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n CUTANA Non-Hot Start 2X PCR Master Mix for CUT&Tag is specifically\n formulated to amplify next generation sequencing (NGS) libraries\n from CUTANA™ pAG-Tn5 tagmented DNA generated during CUT&Tag\n (EpiCypher\n <a href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\">15-1017</a>) [<a\n href=\"https://pubmed.ncbi.nlm.nih.gov/31036827/\" target=\"_blank\">Kaya-Okur et al., 2019</a>]. This\n ready-to-use master mix only requires the addition of\n primers and the tagmented chromatin to achieve high fidelity\n amplification of NGS libraries.\n </p>\n <p>\n <strong>NOTE:</strong> this product was previously named \"CUTANA™\n High Fidelity 2X PCR Master Mix\"\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/proteins/15-1018-next-gen-sequencing.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"15-1018-next-gen-sequencing\"\n src=\"/content/images/products/proteins/15-1018-next-gen-sequencing.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: CUT&Tag Next-Generation Sequencing (NGS)\n libraries</strong><br />\n CUT&Tag was performed as described in <strong>Figure 3</strong>. Recovered DNA was\n directly PCR amplified to produce sequence-ready libraries.\n Agilent TapeStation traces for libraries derived from\n negative control Rabbit IgG (top) and H3K27me3 (bottom) are\n shown. Excised DNA is highly enriched for mononucleosomes\n (peak at ~300 bp reflects ~150 bp insert size).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/proteins/15-1018-next-gen-sequencing-data.jpeg\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"15-1018-next-gen-sequencing-data\"\n src=\"/content/images/products/proteins/15-1018-next-gen-sequencing-data.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 2: CUT&Tag NGS data</strong><br />\n CUT&Tag was performed as described in <strong>Figure 3</strong>. A 116 kb\n representative locus is shown for IgG, H3K4me3, or H3K27me3\n antibodies. NGS libraries amplified using the CUTANA Non-Hot\n Start 2X PCR Master Mix represented the expected genomic\n distribution of H3K4me3 and H3K27me3. Image was generated\n using the Integrative Genomics Viewer (IGV, Broad\n Institute).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/methods/cut-and-tag-methods.png\" target=\"_blank\"\n class=\"image-picker__main-image-link\"><img loading=\"lazy\" alt=\"cut-and-tag-methods\"\n src=\"/content/images/products/methods/cut-and-tag-methods.png\" class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 3: CUT&Tag methods</strong><br />\n CUT&Tag was performed on 100k K562 nuclei with 0.5 µg of\n either IgG (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\">13-0042</a>),\n H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\">13-0041</a>),\n or H3K27me3 (ThermoFisher MA5-11198) antibodies\n following the EpiCypher Direct-to-PCR CUT&Tag\n <a href=\"/protocols/\">protocol</a>. CUT&Tag DNA was directly\n PCR amplified using CUTANA Non-Hot Start 2X PCR Master Mix\n to produce NGS libraries. Libraries were run on an Illumina\n NextSeq2000 with paired-end sequencing (2x50 bp). Sample\n sequencing depth was 0.3 million reads (IgG), 1.7 million\n reads (H3K4me3), and 3.8 million reads (H3K27me3). Data were\n aligned to the hg19 genome using Bowtie2. Data were filtered\n to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List\n regions.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\" alt=\"15-1018-next-gen-sequencing\"\n src=\"/content/images/products/proteins/15-1018-next-gen-sequencing.jpeg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" />\n <img loading=\"lazy\" alt=\"15-1018-next-gen-sequencing-data\"\n src=\"/content/images/products/proteins/15-1018-next-gen-sequencing-data.jpeg\"\n class=\"image-picker__side-image\" role=\"button\" />\n <img loading=\"lazy\" alt=\"cut-and-tag-methods\"\n src=\"/content/images/products/methods/cut-and-tag-methods.png\" class=\"image-picker__side-image\"\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 6 months at -20°C from date of receipt. The\n master mix should be completely thawed on ice and resuspended\n prior to use.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n A high fidelity thermostable DNA polymerase with 3’→5’\n exonuclease activity fused to a processivity-enhancing domain in\n a 2x master mix containing 200 µM dNTPs and 4 mM Mg<sup>2+</sup>\n in a proprietary buffer.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n <strong>Recommended use</strong>: For detailed instructions\n regarding non-hot start PCR cycling conditions in CUT&Tag, see\n CUTANA™ Direct-to-PCR CUT&Tag\n <a href=\"/resources/protocols/\">protocol</a>.\n </p>\n\n <p>\n <strong>NOTE</strong>: Not recommended for use with primers or\n templates containing uracil.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/15-1018.pdf\" target=\"_blank\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0030 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 id=\"bioz\" class=\"sub-title1\">Product References</h3>\n\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <object id=\"wobj-3835-15-1018-q\" type=\"text/html\" data=\"https://www.bioz.com/v_widget_6_0/3835/15-1018/\"\n style=\"width: 100%; height: 193px\"></object>\n <div id=\"bioz-w-pb-3835-15-1018-q-div\" style=\"width: 100%\">\n <a id=\"bioz-w-pb-3835-15-1018-q\" style=\"font-size: 12px; text-decoration: none; color: #4698cb\"\n href=\"https://www.bioz.com/\" target=\"_blank\"><img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \" />\n Powered by Bioz</a>\n <a style=\"font-size: 12px; text-decoration: none; float: right; color: transparent\"\n href=\"https://www.bioz.com/result/15-1018/product/EpiCypher/?cn=15-1018\" target=\"_blank\">\n See more details on Bioz</a>\n </div>\n </div>\n </div>\n </div>\n <script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj('s', [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n '15-1018',\n '15-1018'\n );\n });\n </script>\n </div>\n</div>\n<style>\n .bioz-w-header td {\n padding: 0;\n }\n\n td table {\n margin: 0;\n padding: 6px !important;\n }\n</style>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$145.00","value":145,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag ","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":986,"sku":null,"name":"CUTANA™ CUT&Tag Kit","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/CUTANA™ CUT&Tag Antibodies","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/986/1213/CTK20__46720.1713641110.png?c=2","alt":"CUTANA™ CUT&Tag Kit"}],"date_added":"8th Mar 2023","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1219,"name":"Pack Size","value":"48 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$2,695.00","value":2695},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=986"},{"id":764,"sku":null,"name":"CUTANA™ pAG-Tn5 for CUT&Tag","url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"summary":"\n \n \n Type:  Transposase\n \n \n Mol Wgt:  191 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/764/750/2020_CUTTag_icon_RGB_Andy_L__93238.1592491196.png?c=2","alt":"CUTANA™ pAG-Tn5 for CUT&Tag"}],"date_added":"17th Jun 2020","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1005,"name":"Internal Comment","value":"extra boxes in bottom shelf of Venom"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$795.00","value":795},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$795.00","value":795},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$2,995.00","value":2995},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=764"}],"shipping_messages":[],"rating":0,"meta_keywords":"CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag , pcr mix, CUT&Tag, cut and tag, cleavage under targets and tagmentation, chromatin immunocleavage, ChIC, 15-1018","show_quantity_input":1,"title":"CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/753/752/DNA_Vector_Draw_Final-w-border-01__76823.1591894876.1280.1280__20924.1594475393.jpg?c=2","alt":"CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag"}]} Pack Size: 50 reactions

Type: Polymerase
Source: E. coli

Epitope Tag: None

Description

CUTANA Non-Hot Start 2X PCR Master Mix for CUT&Tag is specifically formulated to amplify next generation sequencing (NGS) libraries from CUTANA™ pAG-Tn5 tagmented DNA generated during CUT&Tag (EpiCypher 15-1017) [Kaya-Okur et al., 2019]. This ready-to-use master mix only requires the addition of primers and the tagmented chromatin to achieve high fidelity amplification of NGS libraries.

NOTE: this product was previously named "CUTANA™ High Fidelity 2X PCR Master Mix"

Validation Data

(Click to enlarge)

Figure 1: CUT&Tag Next-Generation Sequencing (NGS) libraries
CUT&Tag was performed as described in Figure 3. Recovered DNA was directly PCR amplified to produce sequence-ready libraries. Agilent TapeStation traces for libraries derived from negative control Rabbit IgG (top) and H3K27me3 (bottom) are shown. Excised DNA is highly enriched for mononucleosomes (peak at ~300 bp reflects ~150 bp insert size).

(Click to enlarge)

Figure 2: CUT&Tag NGS data
CUT&Tag was performed as described in Figure 3. A 116 kb representative locus is shown for IgG, H3K4me3, or H3K27me3 antibodies. NGS libraries amplified using the CUTANA Non-Hot Start 2X PCR Master Mix represented the expected genomic distribution of H3K4me3 and H3K27me3. Image was generated using the Integrative Genomics Viewer (IGV, Broad Institute).

(Click to enlarge)

Figure 3: CUT&Tag methods
CUT&Tag was performed on 100k K562 nuclei with 0.5 µg of either IgG (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), or H3K27me3 (ThermoFisher MA5-11198) antibodies following the EpiCypher Direct-to-PCR CUT&Tag protocol. CUT&Tag DNA was directly PCR amplified using CUTANA Non-Hot Start 2X PCR Master Mix to produce NGS libraries. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample sequencing depth was 0.3 million reads (IgG), 1.7 million reads (H3K4me3), and 3.8 million reads (H3K27me3). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.

Technical Information

Storage

Stable for 6 months at -20°C from date of receipt. The master mix should be completely thawed on ice and resuspended prior to use.

Formulation

A high fidelity thermostable DNA polymerase with 3’→5’ exonuclease activity fused to a processivity-enhancing domain in a 2x master mix containing 200 µM dNTPs and 4 mM Mg²⁺ in a proprietary buffer.