SKU: 13-0037
{"url":"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h4k12ac-antibody-snap-chip-certified","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"id":690,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"","category":["Antibodies/SNAP-ChIP<sup>®</sup> Certified Antibodies"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/690/657/snap-chip-ab__06715.1557259701.1280.1280__28603.1557858601.1280.1280__23520.1557861768.png?c=2","alt":"Histone H4K12ac Antibody - SNAP-ChIP® Certified"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=690","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"450","name":"Pack Size","value":"100 μg"}],"sku":"13-0037","description":"<table border=\"0\" cellpadding=\"2\" cellspacing=\"2\" style=\"width: 100%;\"> <tbody> <tr valign=\"top\"> <td> <table border=\"0\" cellpadding=\"1\" cellspacing=\"1\" style=\"width: 95%;\"> <tbody> <tr> <td> <table border=\"0\" cellpadding=\"0\" cellspacing=\"0\" style=\"width: 100%;\"> <tbody> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Type: </strong>Monoclonal</span></td> \n<td align=\"left\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Host: </strong>Rabbit</span></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Mol Wgt.: </strong>11.4 kDa</span></td> <td align=\"left\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Appl.: </strong>ChIP, ChIP-Seq, WB, ICC, L</span></td> \n</tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Format: </strong>Aff. Pur. IgG</span></td> \n<td align=\"left\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Reactivity: </strong>H, M, WR</span></td> \n</tr> </tbody> </table> </td> </tr> <tr> <td valign=\"top\"> \n <span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Histone H4K12ac Antibody, SNAP-ChIP Certified Description:</strong> This antibody meets EpiCypher’s “<a href=\"https://www.epicypher.com/technologies/snap-chip-spike-ins/certified-antibodies\">SNAP-ChIP<sup>®</sup> Certified</a>” criteria for specificity and efficient target enrichment in a ChIP experiment (<20% cross-reactivity across the panel, >5% recovery of target input). Histone H4 is one of the four proteins that are present in the nucleosome, the basic repeating subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). This antibody reacts to H4K12ac, however its binding to the target is negatively impacted by nearby acetylations (e.g. tetraAc-H4). No cross reactivity\nwith other lysine acylations in the EpiCypher SNAP-ChIP K-AcylStat panel, is detected.</span><br /> \n </td> </tr> \n\n\n\n\n<tr> <td valign=\"top\"> <span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Histone H4K12ac Antibody, SNAP-ChIP Certified Certified Immunogen:</strong></span> <span style=\"font-family:arial,helvetica,sans-serif;\"></span>A synthetic peptide corresponding to histone H4 acetylated at lysine 12.<br />\n </p></td></tr> \n\n\n\n\n\n<tr> <td valign=\"top\"> <span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Histone H4K12ac Antibody, SNAP-ChIP Certified Certified Formulation:</strong></span> <span style=\"font-family:arial,helvetica,sans-serif;\"></span>Protein A affinity-purified recombinant monoclonal antibody (1 mg/mL) in PBS, with 0.09% sodium azide, 1% BSA, and 50% glycerol.<br />\n </p></td></tr>\n\n<tr>\n <td valign=\"top\"> <span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Histone H4K12ac Antibody, SNAP-ChIP Certified Certified Storage and Stability:</strong></span> <span style=\"font-family:arial,helvetica,sans-serif;\">Stable for 1 years at -20°C from date of receipt.</span><br />\n </p></td></tr>\n\n<tr>\n <td valign=\"top\"> <span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Histone H4K12ac Antibody, SNAP-ChIP Certified Certified Application Notes</strong></span> <span style=\"font-family:arial,helvetica,sans-serif;\"><span style=\"font-weight: normal;\"><strong>Recommended dilutions:</strong><br /> \n </span></span>ChIP: 2 - 5 μg per 1x10<sup>6</sup> cells WB: 1 - 2 μg/mL ICC: 0.05 - 2 μg/mL L: 0.25 - 4 μg/mL</td></tr>\n\n<tr>\n <td valign=\"top\"> <p><span style=\"font-family:arial,helvetica,sans-serif;\"><span style=\"font-size: 12pt;\"><strong>Histone H4K12ac Antibody, SNAP-ChIP Certified Certified References:</strong></span></span>\n </p>\n </p>\n <p>Grzybowski et al (2015) Mol Cell 58:886 </p>\n <p>Shah et al (2018) Mol Cell 72:162 </p></td></tr>\n\n<tr>\n <td valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/13-0037.pdf\" target=\"_new\"> <img alt=\"13-0037 Datasheet\" height=\"40\" src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" width=\"30\" /></a></span></td> \n</tr> <tr> <td> <p></p> <p><span style=\"font-family:arial,helvetica,sans-serif;\"><span style=\"font-size: 10pt;\"><strong>Applications Key: ChIP</strong>-Chromatin IP; <strong>E</strong>-ELISA; <strong>FACS</strong>-Flow cytometry; <strong>IF</strong>-Immunofluorescence; <strong>IHC</strong>-Immunohistochemistry; <strong>ICC</strong>-Immunocytochemistry; <strong>L</strong>-Luminex; <strong>IP</strong>-Immunoprecipitation; <strong>WB</strong>-Western Blotting</span></span></p> <p><span style=\"font-family:arial,helvetica,sans-serif;\"><span style=\"font-size: 10pt;\"><strong>Reactivity Key: </strong><strong>B</strong>-Bovine; <strong>Ce</strong>-C. elegans; <strong>Ch</strong>-Chicken; <strong>Dm</strong>- Drosophila; <strong>Eu</strong>-Eukaryote; <strong>H</strong>-Human; <strong>M</strong>-Mouse; <strong>Ma</strong>-Mammal; <strong>R</strong>-Rat; <strong>Sc</strong>-S.cerevesiae; <strong>Sp</strong>-S. pombe; <strong>WR</strong>-Wide Range (predicted); <strong>X</strong>-Xenopus; <strong>Z</strong>-Zebrafish</span></span></p> </td> </tr> </tbody> </table> </td>\n\n<td><table border=\"0\" cellpadding=\"3\" cellspacing=\"3\" style=\"width: 300px;\"> <tbody> \n\n\n<tr> <td align=\"center\"><span style=\"font-family:arial,helvetica,sans-serif;\"><a href=\"https://www.epicypher.com/content/images/products/antibodies/13_0037_SNAP.jpg\" target=\"_new\"><img alt=\"13-0037 SNAP-ChIP Data\" height=\"100\" src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13_0037_SNAP.jpg\" width=\"150\" /></a></span></td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>SNAP-ChIP qPCR Data: </strong>Histone H4K12ac antibody (5 μg) was tested in a\n native ChIP experiment using chromatin from K-562 cells (10 μg)\n with the SNAP-ChIP K-AcylStat Panel (EpiCypher Catalog No. 19-3001)\n spiked-in prior to micrococcal nuclease digestion. Specificity (left Y-axis)was determined by qPCR for the DNA barcodes corresponding to modified\n nucleosomes in the SNAP-ChIP panel (x-axis). Black bar represents\n antibody efficiency (right y-axis; log scale) and indicates percentage of the target immunoprecipitated relative to input. tetraAc-H3 is K4,K9,K14,K18ac; tetraAc-H4 is K5,K8,K12,K16ac; tetraAc-H2A is K5,K8,K13,K15ac <br /> <strong>(Click image to enlarge) </strong></span></p> <p></p> </td> </tr> \n\n<!--BEGIN IMAGE AND CAPTION SECTION-->\n\n<tr> <td align=\"center\"><span style=\"font-family:arial,helvetica,sans-serif;\"><a href=\"https://www.epicypher.com/content/images/products/antibodies/13_0037_luminex.jpg\" target=\"_new\"><img alt=\"13-0037 Luminex Data\" height=\"100\" src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13_0037_luminex.jpg\" width=\"150\" /></a></span></td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Luminex Data:</strong> Histone H4K12ac antibody was assessed using a Luminex® based approach employing dCypher™ Nucleosome K-AcylStat Panel (EpiCypher Catalog No. 16-9003). The panel comprises biotinylated designer nucleosomes (X-Axis) individually coupled to uniquely identifiable Luminex Magplex® beads. Antibody binding to nucleosomes was tested in multiplex (24-plex) at a 1:1000 dilution, and detected with second layer anti-IgG*PE. Data was generated using a Luminex FlexMAP3D®. Data normalized to relevant on-target (H4K12ac; set to 100) is shown. \n\ntetraAc-H3 is K4,K9,K14,K18ac; tetraAc-H4 is K5,K8,K12,K16ac; tetraAc-H2A is K5,K8,K13,K15ac<br /> <strong>(Click image to enlarge) </strong></span></p> <p></p> </td></tr>\n\n\n<!--END IMAGE AND CAPTION SECTION-->\n\n\n\n\n\n\n\n <tr valign=\"top\"> <td align=\"center\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><a href=\"https://www.epicypher.com/content/images/products/antibodies/13_0037_wb_tds.jpg\" target=\"_new\"><img alt=\"13-0037 Western Blot data\" height=\"30\" src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13_0037_wb_tds.jpg\" width=\"50\" /></a></span></td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Western Blot Data: </strong>Western blot of acid extracts from HeLa cells treated with sodium butyrate ( 1 ), acid extracts from untreated HeLa cells ( 2 ) using EpiCypher Catalog No. 13-0037 at 0.5 µg/mL.<br /> <strong>(Click image to enlarge) </strong></span></p> <p></p> </td> </tr> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"><span style=\"font-family:arial,helvetica,sans-serif;\"><a href=\"https://www.epicypher.com/content/images/products/antibodies/13_0037_ICC_tds.jpg\" target=\"_new\"><img alt=\"13-0037 ICC\" height=\"100\" src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13_0037_ICC_tds.jpg\" width=\"127\" /></a></span></td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family:arial,helvetica,sans-serif;\"><strong>Immunocytochemistry Data: </strong>Immunocytochemistry of HeLa cells treated with sodium butyrate, using H4K12ac antibody, EpiCypher Catalog No. 13-0037 (red). Actin filaments have been labeled with fluorescein phalloidin (green).<br /> <strong>(Click image to enlarge) </strong></span></p> <p></p> </td> </tr> </tbody> </table> </td> \n</tr>\n\n\n </tbody> </table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$515.00","value":515,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":684,"sku":"13-0035","name":"Histone H3K36ac Antibody, SNAP-ChIP® Certified","url":"https://www.epicypher.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k36ac-antibody-snap-chip-certified","availability":"","rating":null,"brand":{"name":null},"category":["Antibodies/SNAP-ChIP<sup>®</sup> Certified Antibodies"],"summary":" Type: 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Pack Size: 100 μg
