CUTANA™ DNA Purification Kit DISCONTINUED

$265.00

SKU: 14-0050
{"url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-dna-purification-kit","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"max_purchase_quantity":0,"id":773,"bulk_discount_rates":[],"can_purchase":false,"meta_description":"CUTANA™ DNA Purification Kit is validated for CUT&RUN DNA isolation and purification. Provides all necessary components for 50 samples.","category":[],"meta_keywords":"DNA purification, DNA isolation, DNA extraction, CUT&RUN DNA, CUTANA DNA Purification kit, ","AddThisServiceButtonMeta":"","images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/773/999/Epicypher_CUTANA_DNA_Purification_Box__48142.1646406779.png?c=2","alt":"CUTANA™ DNA Purification Kit *DISCONTINUED*"}],"main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/773/999/Epicypher_CUTANA_DNA_Purification_Box__48142.1646406779.png?c=2","alt":"CUTANA™ DNA Purification Kit *DISCONTINUED*"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=773","shipping":[],"num_reviews":0,"weight":"0.10 LBS","custom_fields":[{"id":"1311","name":"Pack size","value":"50 samples"}],"sku":"14-0050","description":"<div class=\"product-general-info\">\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div id='w-s-3835-14-0050' style=\"width:240px;height:58px;position:relative;overflow-y: hidden\"></div>\n \n </a>\n </li>\n </ul>\n</div>\n\n<a style=\"color: #fff\" href=\"/products/epigenetics-kits-and-reagents/cutana-quick-cleanup-dna-purification-kit\">\n <p style=\"\n background-color: #4698cb;\n color: #fff;\n padding: 1.3rem;\n text-align: center;\n border-radius: 12px;\n margin-top: 2.5rem;\n \">\n Product Discontinued - we recommend our bead-based DNA Purification Kit (14-0052)\n </p>\n</a>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to\n specifically release genomic fragments of interest into solution, enabling next-generation sequencing\n and genomic mapping with unprecedented sensitivity. High yield purification and concentration of\n partitioned chromatin DNA is an essential step - one that requires a workflow specifically compatible\n with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low\n as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation\n sequencing (<strong>Figure 1</strong>). The user-friendly workflow can be completed in just 30 minutes.\n The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of\n CUT&RUN targets, including histone PTMs and transcription factors.\n </p>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a href=\"/content/images/products/kits/14-0050-dna-fragment-size-distribution-analysis.jpeg\"\n target=\"_new\" class=\"image-picker__main-image-link\"><img loading=\"lazy\"\n alt=\"14-0050-dna-fragment-size-distribution-analysis\"\n src=\"/content/images/products/kits/14-0050-dna-fragment-size-distribution-analysis.jpeg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\">(Click to enlarge)</span></a>\n <button class=\"image-picker__right-arrow\">\n <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\">\n <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"><strong>Figure 1: CUT&RUN DNA fragment size distribution\n analysis</strong><br />\n 500,000 K562 cells per sample were processed with the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher\n <a href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\">14-1048</a>)\n following treatment with IgG negative control (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\">13-0042</a>),\n H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\">13-0041</a>),\n H3K27me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k27me3-antibody-snap-chip-certified-cutana-cut-run-compatible-discontinued\">13-0030</a>)\n and CTCF (EpiCypher\n <a\n href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ctcf-cutana-cut-and-run-antibody\">13-2014</a>)\n antibodies. Isolated DNA was used to prepare paired-end Illumina<sup>®</sup> sequencing\n libraries, which were analyzed by Agilent TapeStation<sup>®</sup>\n , confirming enrichment of mononucleosomes (~300 bp peak represents ~170 bp excised DNA +\n sequencing adapters).\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img loading=\"lazy\" alt=\"14-0050-dna-fragment-size-distribution-analysis\"\n src=\"/content/images/products/kits/14-0050-dna-fragment-size-distribution-analysis.jpeg\" width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Kit Contents</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p style=\"margin: 0\">50 DNA Cleanup Columns + Collection Tubes</p>\n <p style=\"margin: 0\">DNA Binding Buffer - Add 6.9 mL Isopropanol prior to use</p>\n <p style=\"margin: 0\">DNA Wash Buffer - Add 20 mL ≥ 95% EtOH prior to use</p>\n <p style=\"margin: 0\">DNA Elution Buffer</p>\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Store at room temperature. Stable for 6 months from date of shipment.\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n See\n <a href=\"/resources/protocols/cutana-dna-purification-kit-manual\">kit manual</a>\n for complete instructions.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <h3 style=\"color: #4698cb; font-size: 24px; border-bottom: none\" class=\"custom-title-description\">\n Documents & Resources\n </h3>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n \n <h3 style=\"padding-bottom: 5px; margin-top: 1rem; padding-top: 0\" class=\"sub-title1\">Kit Version 2</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/14-0050.pdf\" target=\"_new\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet </span>\n </a>\n </div>\n <div class=\"product-documents\">\n <a href=\"/content/documents/manuals/dna-purification-kit.pdf\" target=\"_new\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">DNA Purification Kit Manual</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n \n <h3 style=\"padding-bottom: 5px; margin-top: 1rem\" class=\"sub-title1\">Kit Version 1</h3>\n\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>Lots 20226001-53, 20335002, and 21130003-02</p>\n <div class=\"product-documents\">\n <a href=\"/content/documents/tds/14-0050-01.pdf\" target=\"_new\" class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n\n <div class=\"product-documents\">\n <a href=\"/content/documents/manuals/dna-purification-kit_1.0.pdf\" target=\"_new\"\n class=\"product-documents__link\">\n <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" />\n <g>\n <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\">DNA Purification Kit Manual</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 id=\"bioz\" class=\"sub-title1\">Product References</h3>\n\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <object id=\"wobj-3835-14-0050-q\" type=\"text/html\" data=\"https://www.bioz.com/v_widget_6_0/3835/14-0050/\"\n style=\"width: 100%; height: 193px\"></object>\n <div id=\"bioz-w-pb-3835-14-0050-q-div\" style=\"width: 100%\">\n <a id=\"bioz-w-pb-3835-14-0050-q\" style=\"font-size: 12px; text-decoration: none; color: #4698cb\"\n href=\"https://www.bioz.com/\" target=\"_blank\"><img src=\"https://cdn.bioz.com/assets/favicon.png\" style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n \" />\n Powered by Bioz</a>\n <a style=\"font-size: 12px; text-decoration: none; float: right; color: transparent\"\n href=\"https://www.bioz.com/result/14-0050/product/EpiCypher/?cn=14-0050\" target=\"_blank\">\n See more details on Bioz</a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj('s', [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n '14-0050',\n '14-0050'\n );\n });\n</script>\n<style>\n .bioz-w-header td {\n padding: 0;\n }\n\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n .button-group {\n display: none;\n }\n</style>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$265.00","value":265,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"CUTANA™ DNA Purification Kit","mpn":null,"upc":null,"options":[],"related_products":[{"id":772,"sku":"14-1048","name":"CUTANA™ ChIC/CUT&RUN Kit","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n \n \n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/772/998/Epicypher_CUTANA_CUTRUN_Box__31908.1646406725.png?c=2","alt":"CUTANA™ ChIC/CUT&RUN Kit"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/772/998/Epicypher_CUTANA_CUTRUN_Box__31908.1646406725.png?c=2","alt":"CUTANA™ ChIC/CUT&RUN Kit"}],"date_added":"13th Aug 2020","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":588,"name":"Pack size","value":"48 Reactions"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"add_to_cart_url":"https://www.epicypher.com/cart.php?action=add&product_id=772","price":{"without_tax":{"currency":"USD","formatted":"$945.00","value":945},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=772"},{"id":694,"sku":null,"name":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows","url":"https://www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows","availability":"","rating":null,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays"],"summary":"\n \n \n Type:  Nuclease\n \n \n Mol Wgt:  43.7 kDa\n \n \n \n ","image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"},"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/694/689/Screen_Shot_2020-02-12_at_11.01.55_AM__17144.1581530752.png?c=2","alt":"CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows"}],"date_added":"12th Aug 2019","pre_order":false,"show_cart_action":true,"has_options":true,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":1174,"name":"Internal Comment","value":"Excess in bottom of Venom"},{"id":1175,"name":"Internal Comment","value":"Bulk in Psylocke"}],"num_reviews":null,"weight":{"formatted":"0.01 LBS","value":0.01},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"price_range":{"min":{"without_tax":{"currency":"USD","formatted":"$335.00","value":335},"tax_label":"Sales Tax"},"max":{"without_tax":{"currency":"USD","formatted":"$1,295.00","value":1295},"tax_label":"Sales Tax"}},"tax_label":"Sales Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=694"}],"shipping_messages":[],"rating":0,"title":"CUTANA™ DNA Purification Kit *DISCONTINUED*","gift_wrapping_available":false,"min_purchase_quantity":0} Pack size: 50 samples

Product Discontinued - we recommend our bead-based DNA Purification Kit (14-0052)

Description

Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin DNA is an essential step - one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.

Validation Data

14-0050-dna-fragment-size-distribution-analysis

(Click to enlarge)

Figure 1: CUT&RUN DNA fragment size distribution analysis
500,000 K562 cells per sample were processed with the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher 14-1048) following treatment with IgG negative control (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), H3K27me3 (EpiCypher 13-0030) and CTCF (EpiCypher 13-2014) antibodies. Isolated DNA was used to prepare paired-end Illumina^® sequencing libraries, which were analyzed by Agilent TapeStation^® , confirming enrichment of mononucleosomes (~300 bp peak represents ~170 bp excised DNA + sequencing adapters).