SKU: 18-2044
{"url":"https://www.epicypher.com/products/nucleosomes/nucleosome-assembly-601-sequence-dna-199-bp-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":"","id":"868","bulk_discount_rates":[],"can_purchase":true,"meta_description":"Nucleosomal template DNA with 26 bp extended linkers serves as a DNA-only control when paired with Methyl DNA recombinant nucleosomes. ","category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits","Nucleosomes/Methyl DNA Designer Nucleosomes"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/868/945/nucleosome-assembly-601-sequence-dna-199-bp-biotinylated__63988.1645734536.jpg?c=2","alt":"Nucleosome Assembly 601 Sequence DNA, 199 bp, Biotinylated"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=868","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"851","name":"Pack Size","value":"50 µg"}],"sku":"18-2044","description":"<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n Nucleosome Assembly 601 Sequence DNA, 199 bp, Biotinylated is a\n double-stranded DNA fragment with 26 bp linkers on either end of a 147\n bp 601 Widom sequence, identified by Lowary and Widom using the SELEX\n method [1]. The 601 sequence DNA has high affinity for histone\n octamers and is useful for <em>in vitro</em> nucleosome assembly.\n There is a biotin group on the 5’ end of the fragment, which makes it\n ideal for use in nucleosome binding assays and pull-down experiments.\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description-specific\n \"\n >\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div\n class=\"\n image-picker__main-content_active image-picker__main-content\n \"\n >\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\"\n />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/18-2044-dna-gel-data.jpeg\"\n target=\"_blank\"\n class=\"image-picker__main-image-link\"\n ><img\n alt=\"18-2044-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/18-2044-dna-gel-data.jpeg\"\n class=\"image-picker__main-image\"\n />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\"\n >\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\"\n />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong>Figure 1: DNA Gel Data</strong><br />\n Nucleosome Assembly 601 Sequence DNA, 199 bp, Biotinylated\n (100 ng) resolved via native PAGE gel and stained with\n ethidium bromide. Migration positions of DNA molecular\n weight markers are indicated.\n </span>\n </p>\n </div>\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img alt=\"18-2044-dna-gel-data\"\n src=\"/content/images/products/nucleosomes/18-2044-dna-gel-data.jpeg\"\n class=\" image-picker__side-image image-picker__side-image_active\n role=\"button\" />\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Formulation</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n 50 µg lyophilized 601 sequence DNA\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage and Stability</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Stable for 2 years at -20°C from date of receipt. After\n resuspending, aliquots should be stored at -80°C.\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Application Notes</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <p>\n This 199 bp biotinylated 601 sequence DNA is useful for assembly of\n nucleosomes with purified or recombinant histone octamers (Catalog\n No.\n <a href=\"/products/nucleosomes/histone-octamer-recombinant-human\"\n >16-0001</a\n >). See Luger <em>et al.</em> (1999) for recommended nucleosome\n reconstitution protocol [2]. This product also serves as a DNA only\n control for experiments utilizing the 199x601 nucleosome (Catalog\n No.\n <a\n href=\"/products/nucleosomes/mononucleosomes-recombinant-199x601-dna-biotinylated\"\n >16-2044</a\n >). These products are part of the\n <a href=\"/products/nucleosomes/methyl-dna-designer-nucleosomes\"\n >Methyl DNA designer nuclesosome set</a\n >.\n </p>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">References</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <strong>Background References:</strong>\n\n <br />\n [1] Lowary & Widom <em>J Mol Biol</em> (1998). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\"\n title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\"\n target=\"_blank\"\n >9514715</a\n >\n\n <br />\n [2] Luger <em>et al.</em> <em>Methods Enzymol</em> (1999). PMID:\n <a\n href=\"https://pubmed.ncbi.nlm.nih.gov/10372352/\"\n title=\"Preparation of nucleosome core particle from recombinant histones\"\n target=\"_blank\"\n >10372352</a\n >\n\n <br />\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Documents & Resources</h3>\n <div\n class=\"\n ProductDescriptionContainer\n product-droppdown__section-description\n \"\n >\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/18-2044.pdf\"\n target=\"_new\"\n class=\"product-documents__link\"\n >\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"PDF File\"\n >\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\"\n />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\"\n />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\"\n />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\"\n />\n </g>\n </svg>\n <span class=\"product-documents__info\">Technical Datasheet</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n </div>\n</div>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$115.00","value":115,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"Unmethylated 601 Sequence DNA, 199 bp Biotinylated","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":"","upc":null,"options":[],"related_products":[{"id":103,"sku":"18-0005","name":"Nucleosome Assembly 601 Sequence DNA, 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Pack Size: 50 µg
